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The map label for this gene is sppA [C]

Identifier: 124022325

GI number: 124022325

Start: 578927

End: 579739

Strand: Reverse

Name: sppA [C]

Synonym: P9303_06151

Alternate gene names: 124022325

Gene position: 579739-578927 (Counterclockwise)

Preceding gene: 124022328

Following gene: 124022324

Centisome position: 21.61

GC content: 55.1

Gene sequence:

>813_bases
ATGGGCTGGCTCTGGCGACGCAAATCTCGCCGAAGGATCGCACACATCAGCATTGACGGAGCAATCAGCGGTGCAACGCG
CGAGCGGGTGCTGAAAGCGATCAAGGAAGTTGAAGAGAGAGAGTTTCCCGCTCTTCTGCTGCGCATCGACAGCCCAGGAG
GAACTGTCGGTGACAGTCAGGAAATCCATGCGGCCCTGCTCAGGCTTAGAGAAAAGGGTTGTCATGTCGTAGCAAGCTTC
GGCAATGTATCCGCCTCCGGTGGTGTGTATGTCGGGGTAGCTGCAGAAAAAATTGTTGCCAATCCCGGCACAATCACTGG
CTCAATCGGGGTCATCTTGCGGGGCAACAACCTCTCCAAATTGCTGGAGAGAATCGGCATCCGCTTTGAGACAGTAAAAA
GCGGCACCTACAAGGACATTCTCTCGCCTGATCGCGCACTCACAGCAGAGGAGCGTCAGCTACTGCAATCACTGATCGAT
AGCAGCTATGAGCAATTTGTGAACGCGGTAGCAGAAGGCCGCCATCTCAGTGCCGAAGAGGTGCGCAACTTCGCCGACGG
CCGCGTGTTCAGCGGAGCTCAGGCCCATGAACTCGGACTCATCGATGAGCTGGGAGATGAAGAACATGCTCGAAAACTTG
CGGCCAAGCTGGCGGACCTTGATGAAGCCAACACCCAAACGCTCAAACTGGGACGCCCCAAAAAACGGCTAGCGGGATTT
CTACCAGGCAGCAAACTCCTATCCAAACTTGCAGAGCTTCTCAACCTTGAGCTTGGCAATAGTGGTCAGGTGCTCTGGCT
CTTTCTGCCATGA

Upstream 100 bases:

>100_bases
AGGGCAAAGGGCAGGACCATCAACAGCCAGTGCCGTATTGACAACATGAAGGCAACAAGCAGGTTCCTTTTTAAGATCCA
ATCCTCTACGAAGAAATTGA

Downstream 100 bases:

>100_bases
CCACTGCCATCCCTTATGGAGGCCTCCACTTACACGGGCTCAGAGGTGCCACAACAAGCTCTGCCAACACGACCAAAGCC
ATCGAGGCAGCCGTCAGCGA

Product: signal peptide peptidase SppA (protease IV)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MGWLWRRKSRRRIAHISIDGAISGATRERVLKAIKEVEEREFPALLLRIDSPGGTVGDSQEIHAALLRLREKGCHVVASF
GNVSASGGVYVGVAAEKIVANPGTITGSIGVILRGNNLSKLLERIGIRFETVKSGTYKDILSPDRALTAEERQLLQSLID
SSYEQFVNAVAEGRHLSAEEVRNFADGRVFSGAQAHELGLIDELGDEEHARKLAAKLADLDEANTQTLKLGRPKKRLAGF
LPGSKLLSKLAELLNLELGNSGQVLWLFLP

Sequences:

>Translated_270_residues
MGWLWRRKSRRRIAHISIDGAISGATRERVLKAIKEVEEREFPALLLRIDSPGGTVGDSQEIHAALLRLREKGCHVVASF
GNVSASGGVYVGVAAEKIVANPGTITGSIGVILRGNNLSKLLERIGIRFETVKSGTYKDILSPDRALTAEERQLLQSLID
SSYEQFVNAVAEGRHLSAEEVRNFADGRVFSGAQAHELGLIDELGDEEHARKLAAKLADLDEANTQTLKLGRPKKRLAGF
LPGSKLLSKLAELLNLELGNSGQVLWLFLP
>Mature_269_residues
GWLWRRKSRRRIAHISIDGAISGATRERVLKAIKEVEEREFPALLLRIDSPGGTVGDSQEIHAALLRLREKGCHVVASFG
NVSASGGVYVGVAAEKIVANPGTITGSIGVILRGNNLSKLLERIGIRFETVKSGTYKDILSPDRALTAEERQLLQSLIDS
SYEQFVNAVAEGRHLSAEEVRNFADGRVFSGAQAHELGLIDELGDEEHARKLAAKLADLDEANTQTLKLGRPKKRLAGFL
PGSKLLSKLAELLNLELGNSGQVLWLFLP

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=179, Percent_Identity=38.5474860335196, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787527, Length=189, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 29406; Mature: 29275

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWLWRRKSRRRIAHISIDGAISGATRERVLKAIKEVEEREFPALLLRIDSPGGTVGDSQ
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCH
EIHAALLRLREKGCHVVASFGNVSASGGVYVGVAAEKIVANPGTITGSIGVILRGNNLSK
HHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEHHHHCCCCCCEEECEEEEEECCCHHH
LLERIGIRFETVKSGTYKDILSPDRALTAEERQLLQSLIDSSYEQFVNAVAEGRHLSAEE
HHHHHCCEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VRNFADGRVFSGAQAHELGLIDELGDEEHARKLAAKLADLDEANTQTLKLGRPKKRLAGF
HHHHHCCCEECCCCHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHC
LPGSKLLSKLAELLNLELGNSGQVLWLFLP
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
GWLWRRKSRRRIAHISIDGAISGATRERVLKAIKEVEEREFPALLLRIDSPGGTVGDSQ
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCH
EIHAALLRLREKGCHVVASFGNVSASGGVYVGVAAEKIVANPGTITGSIGVILRGNNLSK
HHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEHHHHCCCCCCEEECEEEEEECCCHHH
LLERIGIRFETVKSGTYKDILSPDRALTAEERQLLQSLIDSSYEQFVNAVAEGRHLSAEE
HHHHHCCEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VRNFADGRVFSGAQAHELGLIDELGDEEHARKLAAKLADLDEANTQTLKLGRPKKRLAGF
HHHHHCCCEECCCCHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHC
LPGSKLLSKLAELLNLELGNSGQVLWLFLP
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]