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The map label for this gene is tyrS [H]

Identifier: 124022271

GI number: 124022271

Start: 542039

End: 543265

Strand: Direct

Name: tyrS [H]

Synonym: P9303_05611

Alternate gene names: 124022271

Gene position: 542039-543265 (Clockwise)

Preceding gene: 124022270

Following gene: 124022272

Centisome position: 20.21

GC content: 54.2

Gene sequence:

>1227_bases
ATGCCGGCCTGGTTGGAGCGGGGCATGGTTGACTTGTTCCCTTCAGGCCAATGGGGGGATGCTGATCAGCAACTTGCGAC
TCGTCTTGATGAGGCTCGAGAACAAAACCGACCCCTGAGGGTCAAGCTTGGTATTGATCCCACTGGTCGTGATATCCATC
TGGGACACAGCATCCTGTTTCGAAAACTGAGAGCTTTTCAGGATGCGGGGCATACAGCAGTGTTGATTATTGGCGATTTC
ACTGCACGAATCGGTGATCCAACCGGCAAGAGCAGCACCAGAGTGCAGCTCACCTCTGAGCAGATTGAGGCTAATGCGGC
GACCTATCTTTCCCAGCTGGGTCAGGGGCAGTCAGCGGAAAAAGCGCTGCTCGATTTCACAACCCCTGGGCGCCTGGAAG
TGCGACGTAATACTGAGTGGTTGGCTGACTTGGATTTGCCCGAGGTGATTGGTTTGTTGGGCACTGCCACGGTTGGGCAA
ATGCTGGCTAAAGAAGACTTTGGCAATCGTTATGGCTCAGGTATTCCGATAGCTCTGCATGAGTTTCTTTATCCCCTCTT
GCAGGGTTACGACTCGGTAGCGGTGCAGGCAGATGTTGAACTTGGGGGGACTGATCAGAAATTCAATGTCGCCATGGGGC
GGGATCTTCAGCGTCATTTCGATCAGCGCCCCCAGTTTGGGCTCCTACTCCCAATTCTGGCTGGCCTGGATGGGGTGCAG
AAGATGAGCAAGAGCCTCTCTAACACTGTGGGACTGAATGAGGACCCCCTTTCCATGTATTCCAAGTTGGAGAAGATTCC
TGATGCCCTTGTAAGCAGTTACGTGGTTTTGCTTACCGATCTCGACCCTGAAGCTTTACCTGTCAATCCGCGTGAACGGC
AGAAGGCCATGGCCCTTGCGGTCACGGCCAGCCGTCATGGCATCGCGGCGGCTGAAGCCGCCCAGAATGATGCAGCCAGA
TTGGTTTCCGGCTCTCAAGATGATGCGGCATCTGTGCCAGAAGCGTTGCTTGCGGATGTGAATTTTCCGGCCAAGGCCTT
TTATCTGCTTAGTGCGATTGGCCTTTGTGCGAGCAGTAGCGAGGCTCGGCGTCAAATCAAGGGTGGTGCGGTACGCCTGG
ATGGCGAAAAAATTACCGATCCCAATTTGGAATTCACTGACTCCAGCCTGCTGATGGGCAAGGTGCTGCAAGTGGGCAAG
AAAACCTTTCGTCGTTTGACGCGTTGA

Upstream 100 bases:

>100_bases
TTGAGCAGGACTCTCGATGAAAGTGATGACTGGCTGACTCGCTACCCGTACGATCCGCTCAGCTAGAGATCCTCTAACGA
TGCCGGAGACGCCGATCACC

Downstream 100 bases:

>100_bases
GTAAAGGACCAACCCCTGCTGTGATGACCTCGCCATTGACTCCTGCCGATCAACTGATCATTGCTCTTGATGGCATGGAG
CGGGCTGAGGCCCTTGCATT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPAWLERGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILFRKLRAFQDAGHTAVLIIGDF
TARIGDPTGKSSTRVQLTSEQIEANAATYLSQLGQGQSAEKALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQ
MLAKEDFGNRYGSGIPIALHEFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
KMSKSLSNTVGLNEDPLSMYSKLEKIPDALVSSYVVLLTDLDPEALPVNPRERQKAMALAVTASRHGIAAAEAAQNDAAR
LVSGSQDDAASVPEALLADVNFPAKAFYLLSAIGLCASSSEARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGK
KTFRRLTR

Sequences:

>Translated_408_residues
MPAWLERGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILFRKLRAFQDAGHTAVLIIGDF
TARIGDPTGKSSTRVQLTSEQIEANAATYLSQLGQGQSAEKALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQ
MLAKEDFGNRYGSGIPIALHEFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
KMSKSLSNTVGLNEDPLSMYSKLEKIPDALVSSYVVLLTDLDPEALPVNPRERQKAMALAVTASRHGIAAAEAAQNDAAR
LVSGSQDDAASVPEALLADVNFPAKAFYLLSAIGLCASSSEARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGK
KTFRRLTR
>Mature_407_residues
PAWLERGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILFRKLRAFQDAGHTAVLIIGDFT
ARIGDPTGKSSTRVQLTSEQIEANAATYLSQLGQGQSAEKALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQM
LAKEDFGNRYGSGIPIALHEFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQK
MSKSLSNTVGLNEDPLSMYSKLEKIPDALVSSYVVLLTDLDPEALPVNPRERQKAMALAVTASRHGIAAAEAAQNDAARL
VSGSQDDAASVPEALLADVNFPAKAFYLLSAIGLCASSSEARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGKK
TFRRLTR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=28.0397022332506, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787925, Length=428, Percent_Identity=29.2056074766355, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=319, Percent_Identity=24.4514106583072, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325160, Length=412, Percent_Identity=25.2427184466019, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=25.8706467661692, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44288; Mature: 44157

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAWLERGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILF
CCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHH
RKLRAFQDAGHTAVLIIGDFTARIGDPTGKSSTRVQLTSEQIEANAATYLSQLGQGQSAE
HHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHH
KALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQMLAKEDFGNRYGSGIPIALH
HHHHCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
HHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
KMSKSLSNTVGLNEDPLSMYSKLEKIPDALVSSYVVLLTDLDPEALPVNPRERQKAMALA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHE
VTASRHGIAAAEAAQNDAARLVSGSQDDAASVPEALLADVNFPAKAFYLLSAIGLCASSS
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
EARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGKKTFRRLTR
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAWLERGMVDLFPSGQWGDADQQLATRLDEAREQNRPLRVKLGIDPTGRDIHLGHSILF
CCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHH
RKLRAFQDAGHTAVLIIGDFTARIGDPTGKSSTRVQLTSEQIEANAATYLSQLGQGQSAE
HHHHHHHCCCCEEEEEEECCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHH
KALLDFTTPGRLEVRRNTEWLADLDLPEVIGLLGTATVGQMLAKEDFGNRYGSGIPIALH
HHHHCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EFLYPLLQGYDSVAVQADVELGGTDQKFNVAMGRDLQRHFDQRPQFGLLLPILAGLDGVQ
HHHHHHHCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
KMSKSLSNTVGLNEDPLSMYSKLEKIPDALVSSYVVLLTDLDPEALPVNPRERQKAMALA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHE
VTASRHGIAAAEAAQNDAARLVSGSQDDAASVPEALLADVNFPAKAFYLLSAIGLCASSS
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
EARRQIKGGAVRLDGEKITDPNLEFTDSSLLMGKVLQVGKKTFRRLTR
HHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]