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The map label for this gene is queF

Identifier: 124022180

GI number: 124022180

Start: 470192

End: 470599

Strand: Direct

Name: queF

Synonym: P9303_04701

Alternate gene names: 124022180

Gene position: 470192-470599 (Clockwise)

Preceding gene: 124022179

Following gene: 124022185

Centisome position: 17.53

GC content: 52.21

Gene sequence:

>408_bases
GTGAGCAGTACTGAGGCGCAGGCATCTAAACCCCTCTATGGCGAACGGGTGATCGCTGAGGGTGAGTTGATCTGCTTTGA
CAATCCGAGGCCTGAGCGGCCCTATGAGATCTCAATTGAGTTGCCTGAGTTCACCTGTCAATGTCCTTTCTCTGGTTATC
CCGATTTTGCTGTGTTACGTCTGCTCTATCAGCCAGGCCCGAGGGTGATTGAGCTCAAAGCGATCAAGCTCTATGTGAAT
AGCTATCGGAATTGCTCGATTTCACATGAGGAGGCCGCAAACAAGATCCTCGATGATTTGGTCGTCGCCTGTGATCCGGT
CTGGATGCAATTAGAAGCAGATTTCAATCCGCGCGGCAATGTGCATACGGTGGTGCGGGTTAGCCATGGCAGTCGTCAGC
CCTGTTGA

Upstream 100 bases:

>100_bases
GTTTTTGCGGTTTGCGTTGACTCCAAGGCGGATTCTTTCTCCTCTAAGGCCGAGCCATGAGCCGAGATCTAGTGTCCGTT
TTAGGCATCGTTCCATCCCC

Downstream 100 bases:

>100_bases
GTCAATGCTGGGATGCGGGGGTGATCGAAGCAAGCAGGCTGGGGAGCTCATTAGCGAAGCCGGTCAGATTGCGGTTGCAG
AGACCGCCAACATGTAGGCA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MSSTEAQASKPLYGERVIAEGELICFDNPRPERPYEISIELPEFTCQCPFSGYPDFAVLRLLYQPGPRVIELKAIKLYVN
SYRNCSISHEEAANKILDDLVVACDPVWMQLEADFNPRGNVHTVVRVSHGSRQPC

Sequences:

>Translated_135_residues
MSSTEAQASKPLYGERVIAEGELICFDNPRPERPYEISIELPEFTCQCPFSGYPDFAVLRLLYQPGPRVIELKAIKLYVN
SYRNCSISHEEAANKILDDLVVACDPVWMQLEADFNPRGNVHTVVRVSHGSRQPC
>Mature_134_residues
SSTEAQASKPLYGERVIAEGELICFDNPRPERPYEISIELPEFTCQCPFSGYPDFAVLRLLYQPGPRVIELKAIKLYVNS
YRNCSISHEEAANKILDDLVVACDPVWMQLEADFNPRGNVHTVVRVSHGSRQPC

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_PROM3 (A2C6W2)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016487.1
- ProteinModelPortal:   A2C6W2
- SMR:   A2C6W2
- STRING:   A2C6W2
- GeneID:   4778578
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_04701
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 15258; Mature: 15126

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTEAQASKPLYGERVIAEGELICFDNPRPERPYEISIELPEFTCQCPFSGYPDFAVLR
CCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHH
LLYQPGPRVIELKAIKLYVNSYRNCSISHEEAANKILDDLVVACDPVWMQLEADFNPRGN
HHHCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
VHTVVRVSHGSRQPC
EEEEEEECCCCCCCC
>Mature Secondary Structure 
SSTEAQASKPLYGERVIAEGELICFDNPRPERPYEISIELPEFTCQCPFSGYPDFAVLR
CCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHH
LLYQPGPRVIELKAIKLYVNSYRNCSISHEEAANKILDDLVVACDPVWMQLEADFNPRGN
HHHCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
VHTVVRVSHGSRQPC
EEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA