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Identifier: 124022020

GI number: 124022020

Start: 323349

End: 323954

Strand: Direct

Name: def

Synonym: P9303_03091

Alternate gene names: 124022020

Gene position: 323349-323954 (Clockwise)

Preceding gene: 124022019

Following gene: 124022022

Centisome position: 12.05

GC content: 53.63

Gene sequence:

>606_bases
TTGGCTCGAAGCTTCGCTCAGCTGGCACGTACGGCCGAAAAAACCAAGGGCTCTGTTGCAGTCCCGAAGGAGCCGCTTGA
TCATCCGCCTCTGCAAATTCACACGTTGGGCAATGGGGTTTTGCGGCAATCCACCCGTCGGATTGGCAAGGTTGATGAAT
CTGTGAGGGACTTGGTGAGGGACATGCTTCGCAGCATGTACGCAGCTAAGGGGATTGGTTTGGCTGCCCCTCAGGTGGGA
ATTCATAAACAGCTCTTGGTCCTTGATCTTGATCTGGAAACCCCTACAACCCCGCCAGTGGTGTTGATCAATCCCGAGAT
CATTAGCTCCAGTGCAACTGTTGAAACCTATGAGGAAGGCTGCCTGAGCATCCCAGGCGTTTATCTCAATGTGGTGCGGC
CTAGTGAGATCGTGCTCAGTTTTCGCGATGAGATGGGACGACCACGCAAAATGAAGGCCGACGGCCTGATGGCTCGCTGC
ATCCAGCACGAGATGGATCACCTTGAAGGTGTCCTTTTTGTTGACAGGGTCACCGATGAGAATGAGCTCAGCCTTGAGTT
AAAGGAGCATGGCTTCAAGCGAGCTGATGTTCGTCCGCTGGTTTGA

Upstream 100 bases:

>100_bases
TCGGGTTTCGCTGGTGATGGCTGGTGCTTTCCATGGCGGTTTGGGGTCTGATGTGCGGTGGAGTTGCCTAAACTCAATCA
ACTGATGCTGAGACTTTTCT

Downstream 100 bases:

>100_bases
ATGCGTTGGCTGAAGCCTTTGAGGATGCTTTCTTTGACGGTTTCTTGTGTGTTTGGCGTCGCGCTTTGATTGGTTCTTTC
TTTGCGTTGCTTTGAAGCGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MARSFAQLARTAEKTKGSVAVPKEPLDHPPLQIHTLGNGVLRQSTRRIGKVDESVRDLVRDMLRSMYAAKGIGLAAPQVG
IHKQLLVLDLDLETPTTPPVVLINPEIISSSATVETYEEGCLSIPGVYLNVVRPSEIVLSFRDEMGRPRKMKADGLMARC
IQHEMDHLEGVLFVDRVTDENELSLELKEHGFKRADVRPLV

Sequences:

>Translated_201_residues
MARSFAQLARTAEKTKGSVAVPKEPLDHPPLQIHTLGNGVLRQSTRRIGKVDESVRDLVRDMLRSMYAAKGIGLAAPQVG
IHKQLLVLDLDLETPTTPPVVLINPEIISSSATVETYEEGCLSIPGVYLNVVRPSEIVLSFRDEMGRPRKMKADGLMARC
IQHEMDHLEGVLFVDRVTDENELSLELKEHGFKRADVRPLV
>Mature_200_residues
ARSFAQLARTAEKTKGSVAVPKEPLDHPPLQIHTLGNGVLRQSTRRIGKVDESVRDLVRDMLRSMYAAKGIGLAAPQVGI
HKQLLVLDLDLETPTTPPVVLINPEIISSSATVETYEEGCLSIPGVYLNVVRPSEIVLSFRDEMGRPRKMKADGLMARCI
QHEMDHLEGVLFVDRVTDENELSLELKEHGFKRADVRPLV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=173, Percent_Identity=30.0578034682081, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789682, Length=148, Percent_Identity=41.2162162162162, Blast_Score=110, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=29.3413173652695, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645726, Length=192, Percent_Identity=27.0833333333333, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_PROMM (Q7V5F9)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895428.1
- ProteinModelPortal:   Q7V5F9
- SMR:   Q7V5F9
- STRING:   Q7V5F9
- GeneID:   1728260
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1601
- NMPDR:   fig|74547.1.peg.1595
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RITKVDE
- ProtClustDB:   PRK00150
- BioCyc:   PMAR74547:PMT1601-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 22311; Mature: 22180

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSFAQLARTAEKTKGSVAVPKEPLDHPPLQIHTLGNGVLRQSTRRIGKVDESVRDLVR
CCCHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
DMLRSMYAAKGIGLAAPQVGIHKQLLVLDLDLETPTTPPVVLINPEIISSSATVETYEEG
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCCHHHHHCC
CLSIPGVYLNVVRPSEIVLSFRDEMGRPRKMKADGLMARCIQHEMDHLEGVLFVDRVTDE
CCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCC
NELSLELKEHGFKRADVRPLV
CCEEEEHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ARSFAQLARTAEKTKGSVAVPKEPLDHPPLQIHTLGNGVLRQSTRRIGKVDESVRDLVR
CCHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
DMLRSMYAAKGIGLAAPQVGIHKQLLVLDLDLETPTTPPVVLINPEIISSSATVETYEEG
HHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCCHHHHHCC
CLSIPGVYLNVVRPSEIVLSFRDEMGRPRKMKADGLMARCIQHEMDHLEGVLFVDRVTDE
CCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCC
NELSLELKEHGFKRADVRPLV
CCEEEEHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642