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The map label for this gene is thiC [H]

Identifier: 124021910

GI number: 124021910

Start: 215312

End: 216691

Strand: Direct

Name: thiC [H]

Synonym: P9303_01971

Alternate gene names: 124021910

Gene position: 215312-216691 (Clockwise)

Preceding gene: 124021909

Following gene: 124021911

Centisome position: 8.03

GC content: 51.52

Gene sequence:

>1380_bases
ATGCGCGCCTCTTGGGTGGCTGCCCGTAAGGGTCAGGCCAATGTCTCGCAATTGCATTTCGCTCGACAGGGCGTTGTCAC
TCAAGAAATGGACTACGTGGCCAGGCGGGAAAACTTGCCTGAATCGCTTGTCATGGAGGAGGTGGCCCGGGGCAGGATGA
TTATTCCTGCCAACATCAATCATGCAAATTTAGAGCCGATGGCGATTGGTATCGCCTCCAGCTGCAAGGTGAATGCAAAC
ATTGGCGCCTCACCTAACGCCAGTGATGTAGCTGAAGAGCTCAAGAAGCTCGAGCTGGCAGTTAAATATGGCGCAGACAC
CGTGATGGATCTGTCCACTGGAGGGGTCAATCTTGATGAGGTGCGCACGGCGATCATTAATGCTTCACCCGTGCCGATCG
GCACTGTGCCTGTCTATCAGGCGTTGGAAAGCGTGCATGGCTCGATTGAGAAGCTCGACGAAGATGACTTCCTACACATC
ATTGAGAAGCATTGCCAGCAGGGTGTCGACTATCAAACCATTCACGCCGGTTTGTTGATTGAGCACCTTCCGTTGGTGAA
GGGACGCCTGACAGGCATCGTCAGTCGCGGGGGTGGAATTCTTGCTCAGTGGATGCTTTATCACCACAGACAGAACCCTC
TTTTCACCCGCTTTGACGACATCTGCGAGATCTTCAAGCGCTACGACTGCAGTTTTTCACTTGGTGATTCTCTTCGTCCT
GGTTGTCAGCACGATGCTTCTGATGCAGCTCAACTTGCCGAGTTGAAGACCCTTGGAGAATTGACTAAGAGAGCTTGGGC
ACATGACGTGCAGGTGATGGTCGAGGGTCCTGGTCATGTACCAATGGATCAGATCGAATTCAATGTGCGCAAGCAGATGG
AAGAGTGCAATGAGGCACCCTTTTATGTGCTTGGCCCTTTGGTGACAGACATCGCACCGGGTTATGACCACATCACGAGT
GCCATCGGTGCGGCGATGGCAGGCTGGTATGGAACAGCGATGCTTTGTTATGTGACCCCGAAGGAGCATTTGGGTCTGCC
AAACCCTGAGGATGTTCGTGAGGGCTTGATTGCCTACAAAATTGCAGCGCATGCCGCTGACATCGCTCGTCACCGTCCGG
GTGCTCGAGATCGCGATGATGAATTAAGCCGAGCAAGGTACAACTTTGATTGGAACAAACAGTTTGAGCTTTCACTTGAT
CCAGAGCGAGCCAAGCAGTATCACGATGAAACTTTGCCAGCTGACATTTACAAGCAAGCTGAGTTTTGTTCAATGTGTGG
TCCAAAGCATTGTCCAATGCAGACCAAAATTACGGATGAGGATCTAGAAGGTCTCGAAAAATCTCTCAAAAGTAAAGGGA
AAGCTGAGTTGCCAGCTTAG

Upstream 100 bases:

>100_bases
CACCCTCCGAACCTGATACGGGTTGTGCCGGCGCAGGGAAGTGAAATGACGTGATCATTGGTTTTTCGCCAAACCCTGGT
ATCCATTTCGCCTTTTGATC

Downstream 100 bases:

>100_bases
AAGGGTGAAACAATTAAATCATCACTTGCTTCACTCTTATGTGATGTCTGCATAGACAATTCATTTGGCCATGATGCAAT
GAAAAAAACCATTTTAATTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRASWVAARKGQANVSQLHFARQGVVTQEMDYVARRENLPESLVMEEVARGRMIIPANINHANLEPMAIGIASSCKVNAN
IGASPNASDVAEELKKLELAVKYGADTVMDLSTGGVNLDEVRTAIINASPVPIGTVPVYQALESVHGSIEKLDEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPLVKGRLTGIVSRGGGILAQWMLYHHRQNPLFTRFDDICEIFKRYDCSFSLGDSLRP
GCQHDASDAAQLAELKTLGELTKRAWAHDVQVMVEGPGHVPMDQIEFNVRKQMEECNEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYKIAAHAADIARHRPGARDRDDELSRARYNFDWNKQFELSLD
PERAKQYHDETLPADIYKQAEFCSMCGPKHCPMQTKITDEDLEGLEKSLKSKGKAELPA

Sequences:

>Translated_459_residues
MRASWVAARKGQANVSQLHFARQGVVTQEMDYVARRENLPESLVMEEVARGRMIIPANINHANLEPMAIGIASSCKVNAN
IGASPNASDVAEELKKLELAVKYGADTVMDLSTGGVNLDEVRTAIINASPVPIGTVPVYQALESVHGSIEKLDEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPLVKGRLTGIVSRGGGILAQWMLYHHRQNPLFTRFDDICEIFKRYDCSFSLGDSLRP
GCQHDASDAAQLAELKTLGELTKRAWAHDVQVMVEGPGHVPMDQIEFNVRKQMEECNEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYKIAAHAADIARHRPGARDRDDELSRARYNFDWNKQFELSLD
PERAKQYHDETLPADIYKQAEFCSMCGPKHCPMQTKITDEDLEGLEKSLKSKGKAELPA
>Mature_459_residues
MRASWVAARKGQANVSQLHFARQGVVTQEMDYVARRENLPESLVMEEVARGRMIIPANINHANLEPMAIGIASSCKVNAN
IGASPNASDVAEELKKLELAVKYGADTVMDLSTGGVNLDEVRTAIINASPVPIGTVPVYQALESVHGSIEKLDEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPLVKGRLTGIVSRGGGILAQWMLYHHRQNPLFTRFDDICEIFKRYDCSFSLGDSLRP
GCQHDASDAAQLAELKTLGELTKRAWAHDVQVMVEGPGHVPMDQIEFNVRKQMEECNEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYKIAAHAADIARHRPGARDRDDELSRARYNFDWNKQFELSLD
PERAKQYHDETLPADIYKQAEFCSMCGPKHCPMQTKITDEDLEGLEKSLKSKGKAELPA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=458, Percent_Identity=57.4235807860262, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 50834; Mature: 50834

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASWVAARKGQANVSQLHFARQGVVTQEMDYVARRENLPESLVMEEVARGRMIIPANIN
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCC
HANLEPMAIGIASSCKVNANIGASPNASDVAEELKKLELAVKYGADTVMDLSTGGVNLDE
CCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRTAIINASPVPIGTVPVYQALESVHGSIEKLDEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPLVKGRLTGIVSRGGGILAQWMLYHHRQNPLFTRFDDICEIFKRYDCSFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GCQHDASDAAQLAELKTLGELTKRAWAHDVQVMVEGPGHVPMDQIEFNVRKQMEECNEAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
FYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYK
EEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRPGARDRDDELSRARYNFDWNKQFELSLDPERAKQYHDETLPADIYKQA
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKHCPMQTKITDEDLEGLEKSLKSKGKAELPA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRASWVAARKGQANVSQLHFARQGVVTQEMDYVARRENLPESLVMEEVARGRMIIPANIN
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCC
HANLEPMAIGIASSCKVNANIGASPNASDVAEELKKLELAVKYGADTVMDLSTGGVNLDE
CCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRTAIINASPVPIGTVPVYQALESVHGSIEKLDEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPLVKGRLTGIVSRGGGILAQWMLYHHRQNPLFTRFDDICEIFKRYDCSFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GCQHDASDAAQLAELKTLGELTKRAWAHDVQVMVEGPGHVPMDQIEFNVRKQMEECNEAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
FYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYK
EEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRPGARDRDDELSRARYNFDWNKQFELSLDPERAKQYHDETLPADIYKQA
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKHCPMQTKITDEDLEGLEKSLKSKGKAELPA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA