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The map label for this gene is gyrB [H]

Identifier: 124021870

GI number: 124021870

Start: 174585

End: 176552

Strand: Direct

Name: gyrB [H]

Synonym: P9303_01571

Alternate gene names: 124021870

Gene position: 174585-176552 (Clockwise)

Preceding gene: 124021867

Following gene: 124021871

Centisome position: 6.51

GC content: 49.85

Gene sequence:

>1968_bases
ATGAGCGAAGACTCCAAAGTCCAGGCTGCCTATGGCGCCGAACAAATTCAGGTGCTGGAAGGGCTAGAGCCCGTTAGGAA
GCGTCCGGGGATGTACATCGGCTCCACTGGGCCAAGGGGGTTGCATCACCTTGTCTTTGAGGTTGTCGACAACTCTGTTG
ATGAGGCCTTGGCTGGGCACTGTGATGAGATTCTCGTGGTGCTTGGGCCAGATGGTTCCGCATCAATAGCTGACAATGGC
CGTGGCATTCCCACCGACGTTCATTCCCGGACAGGCAAGAGTGCCTTGGAAACGGTGCTCACCGTGCTACATGCTGGCGG
TAAGTTCGGCAGTGGCGGTTACAAGGTCTCTGGCGGCTTGCATGGAGTTGGTGTATCTGTCGTCAATGCCCTGAGTGAAT
GGGTGGAAGTGACGGTGCGGCGTCAAGGACAGGTGCACCGTCAGCGCTTTGAACGTGGGACCGCGATCGGTAGTCTTCGT
TCCGAAGCCCAGCCTGCTTCAGAGAAGGGGCGCACTGGCACGAGCGTTTGTTTTAAGCCGGACGAAGAGATATTTACTGT
TGGCATTGTTTTCGATTACGCCACCCTGTCTGCTCGTTTGCGTGAACTGGCTTATCTCAATGGTGGGGTGCGGATTGTTT
TCCGCGATGAGCGTCAGCCTGTTTTAGATGCTGAAGGGGCTCCTCATGAGGAGGTCTATTTTTATGAGGGCGGTATCAAA
GAATATGTCACCTATATGAATGCGGAAAAGGATGCTCTTCATCCCGAAATTATTTATGTGAATTCTGAAAAGGATAATGT
GCAGGTTGAAGCAGCACTGCAATGGTGTGTTGATGCTTATTCAGACAGCATTCTCGGATTTGCTAATAATATTCGCACTG
TAGATGGGGGTACTCATATTGAGGGGCTGAAGACAGTCCTTACTCGCACTCTCAATACTTTTGCCCGTAAGCGTGGCAAA
CGCAAGGAAGCCGATTCTAATCTGGCTGGAGAAAATATTCGTGAAGGACTTACGGCTGTTCTTTCCGTGAAGGTGCCCGA
TCCGGAATTCGAAGGGCAGACAAAAACCAAGTTGGGCAATACCGAAGTCCGCGGCATTGTCGACAGCCTTGTTGGTGAAG
CATTGAGTCAGTATTTGGAATTTAATCCTGCGGTGATTGATCTTATTCTTGAGAAGGCTATCCAGGCATTCAATGCAGCT
GAAGCTGCACGTCGTGCTCGAGAATTAGTGCGCCGCAAAAGCGTACTTGAGAGTTCTACGTTGCCAGGAAAACTGGCTGA
TTGCAGTTCTAGGGACCCCTCGGAATCAGAGATTTACATCGTGGAGGGCGACTCTGCTGGTGGCTCTGCAAAGCAGGGCC
GAGATCGTCGCTTTCAAGCAATCCTGCCCTTGCGCGGTAAGATCTTAAATATTGAGAAAACAGATGATGCTAAAATATAT
AAAAATACAGAGATCCAGGCGCTGATTACTGCGCTTGGATTAGGCATCAAAGGAGAAGAATTTGATGCAAAAAATCTTAG
ATATCACCGTGTGGTGATCATGACTGATGCTGATGTTGACGGTGCTCATATTCGTACGCTGATACTTACATTTTTCTACC
GCTACCAGAAAGAATTAGTGGAAGGTGGTTATATCTATATTGCGTGCCCTCCTCTTTATAAGGTTGAGCGAGGTAAGAAC
CATACCTATTGCTACAACGAGGCTGATTTACAGACGACACTCTCAGGCTTCCCCGAGAAGGCCAACTACACCATTCAGCG
CTTTAAGGGTCTCGGCGAAATGATGCCAAAGCAGTTGTGGGAGACCACCATGGACCCTTCTACCCGGATGATGAAGCGGG
TGGAGGTTCAGGATGCATTAGAGGCTGATCGTATCTTCACAATTTTGATGGGCGACAAGGTTGCTCCCCGCCGGGAGTTC
ATTGAAACCCACAGCGCTGAGCTCGATATGGCAGCGTTAGATATCTGA

Upstream 100 bases:

>100_bases
AGCGCCAGTGGTGAGCCAGTGGTAAAATGAGCTTGCCAGGTCGAGGTTTCTCGCCGATGAGGCCTCTTAGAACAACCCTT
CCTGGCTGCGGATTTATTGA

Downstream 100 bases:

>100_bases
TGGGTGTTGTGCTCCGTTGGGGGTGGGTCCTAGGGGTGGCCTTGCTAGCTCCTGCAGCCTTGCCGGCTGGTGGGGGTGAG
CGTCGCCAGCCAGAAATTCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSEDSKVQAAYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVFEVVDNSVDEALAGHCDEILVVLGPDGSASIADNG
RGIPTDVHSRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGVSVVNALSEWVEVTVRRQGQVHRQRFERGTAIGSLR
SEAQPASEKGRTGTSVCFKPDEEIFTVGIVFDYATLSARLRELAYLNGGVRIVFRDERQPVLDAEGAPHEEVYFYEGGIK
EYVTYMNAEKDALHPEIIYVNSEKDNVQVEAALQWCVDAYSDSILGFANNIRTVDGGTHIEGLKTVLTRTLNTFARKRGK
RKEADSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGEALSQYLEFNPAVIDLILEKAIQAFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIEKTDDAKIY
KNTEIQALITALGLGIKGEEFDAKNLRYHRVVIMTDADVDGAHIRTLILTFFYRYQKELVEGGYIYIACPPLYKVERGKN
HTYCYNEADLQTTLSGFPEKANYTIQRFKGLGEMMPKQLWETTMDPSTRMMKRVEVQDALEADRIFTILMGDKVAPRREF
IETHSAELDMAALDI

Sequences:

>Translated_655_residues
MSEDSKVQAAYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVFEVVDNSVDEALAGHCDEILVVLGPDGSASIADNG
RGIPTDVHSRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGVSVVNALSEWVEVTVRRQGQVHRQRFERGTAIGSLR
SEAQPASEKGRTGTSVCFKPDEEIFTVGIVFDYATLSARLRELAYLNGGVRIVFRDERQPVLDAEGAPHEEVYFYEGGIK
EYVTYMNAEKDALHPEIIYVNSEKDNVQVEAALQWCVDAYSDSILGFANNIRTVDGGTHIEGLKTVLTRTLNTFARKRGK
RKEADSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGEALSQYLEFNPAVIDLILEKAIQAFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIEKTDDAKIY
KNTEIQALITALGLGIKGEEFDAKNLRYHRVVIMTDADVDGAHIRTLILTFFYRYQKELVEGGYIYIACPPLYKVERGKN
HTYCYNEADLQTTLSGFPEKANYTIQRFKGLGEMMPKQLWETTMDPSTRMMKRVEVQDALEADRIFTILMGDKVAPRREF
IETHSAELDMAALDI
>Mature_654_residues
SEDSKVQAAYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVFEVVDNSVDEALAGHCDEILVVLGPDGSASIADNGR
GIPTDVHSRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGVSVVNALSEWVEVTVRRQGQVHRQRFERGTAIGSLRS
EAQPASEKGRTGTSVCFKPDEEIFTVGIVFDYATLSARLRELAYLNGGVRIVFRDERQPVLDAEGAPHEEVYFYEGGIKE
YVTYMNAEKDALHPEIIYVNSEKDNVQVEAALQWCVDAYSDSILGFANNIRTVDGGTHIEGLKTVLTRTLNTFARKRGKR
KEADSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGEALSQYLEFNPAVIDLILEKAIQAFNAAE
AARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIEKTDDAKIYK
NTEIQALITALGLGIKGEEFDAKNLRYHRVVIMTDADVDGAHIRTLILTFFYRYQKELVEGGYIYIACPPLYKVERGKNH
TYCYNEADLQTTLSGFPEKANYTIQRFKGLGEMMPKQLWETTMDPSTRMMKRVEVQDALEADRIFTILMGDKVAPRREFI
ETHSAELDMAALDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=629, Percent_Identity=24.1653418124006, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19913406, Length=638, Percent_Identity=23.6677115987461, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=53.6672629695885, Blast_Score=577, Evalue=1e-165,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=39.6923076923077, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17535065, Length=626, Percent_Identity=23.482428115016, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=597, Percent_Identity=23.785594639866, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI212645657, Length=180, Percent_Identity=28.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=678, Percent_Identity=24.7787610619469, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17136538, Length=585, Percent_Identity=23.7606837606838, Blast_Score=132, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72273; Mature: 72142

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDSKVQAAYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVFEVVDNSVDEALAGH
CCCCCCEEHHHCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHCC
CDEILVVLGPDGSASIADNGRGIPTDVHSRTGKSALETVLTVLHAGGKFGSGGYKVSGGL
CCEEEEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSEWVEVTVRRQGQVHRQRFERGTAIGSLRSEAQPASEKGRTGTSVCFKP
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCHHCCCCCCEEEECC
DEEIFTVGIVFDYATLSARLRELAYLNGGVRIVFRDERQPVLDAEGAPHEEVYFYEGGIK
CCCEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
EYVTYMNAEKDALHPEIIYVNSEKDNVQVEAALQWCVDAYSDSILGFANNIRTVDGGTHI
HHHHHHCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHCHHHHHHCCCEECCCCCHH
EGLKTVLTRTLNTFARKRGKRKEADSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLVGEALSQYLEFNPAVIDLILEKAIQAFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIEKTDDAKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEE
KNTEIQALITALGLGIKGEEFDAKNLRYHRVVIMTDADVDGAHIRTLILTFFYRYQKELV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EGGYIYIACPPLYKVERGKNHTYCYNEADLQTTLSGFPEKANYTIQRFKGLGEMMPKQLW
CCCEEEEECCCCHHHHCCCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ETTMDPSTRMMKRVEVQDALEADRIFTILMGDKVAPRREFIETHSAELDMAALDI
HHCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure 
SEDSKVQAAYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVFEVVDNSVDEALAGH
CCCCCEEHHHCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHCC
CDEILVVLGPDGSASIADNGRGIPTDVHSRTGKSALETVLTVLHAGGKFGSGGYKVSGGL
CCEEEEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSEWVEVTVRRQGQVHRQRFERGTAIGSLRSEAQPASEKGRTGTSVCFKP
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCHHCCCCCCEEEECC
DEEIFTVGIVFDYATLSARLRELAYLNGGVRIVFRDERQPVLDAEGAPHEEVYFYEGGIK
CCCEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
EYVTYMNAEKDALHPEIIYVNSEKDNVQVEAALQWCVDAYSDSILGFANNIRTVDGGTHI
HHHHHHCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHCHHHHHHCCCEECCCCCHH
EGLKTVLTRTLNTFARKRGKRKEADSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLVGEALSQYLEFNPAVIDLILEKAIQAFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIEKTDDAKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEE
KNTEIQALITALGLGIKGEEFDAKNLRYHRVVIMTDADVDGAHIRTLILTFFYRYQKELV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EGGYIYIACPPLYKVERGKNHTYCYNEADLQTTLSGFPEKANYTIQRFKGLGEMMPKQLW
CCCEEEEECCCCHHHHCCCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ETTMDPSTRMMKRVEVQDALEADRIFTILMGDKVAPRREFIETHSAELDMAALDI
HHCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]