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The map label for this gene is rfbE [H]

Identifier: 124021850

GI number: 124021850

Start: 151389

End: 152093

Strand: Direct

Name: rfbE [H]

Synonym: P9303_01371

Alternate gene names: 124021850

Gene position: 151389-152093 (Clockwise)

Preceding gene: 124021849

Following gene: 124021851

Centisome position: 5.64

GC content: 50.21

Gene sequence:

>705_bases
GTGGGCAGTCGCTCCCTGAAGTCGGCTTTCGTTGGCGGGCTGACAGGGGGTTTGCTCAATCGCAGTCGTACTCGCGGCTT
AACGGTTCAAGCACTTTCTGACTTGAACTTCACTATTCAAAAGGGCGACAGGGTGGCTTTGTTGGGTCATAACGGCGCAG
GAAAGAGCACGTTGTTGCGCTTGCTTGCTGATGTCTATGCACCAAGCCAGGGGCGCATCAGGCGTAATGGACACATTGCC
CCGCTGATTAATAAAAGTTTCTGGGTCGATACTGACCTCAGTGGTCGTCATGCAGCAAGAGCTCAGTACCTATTGAGTCG
AAACACCATTGATGGTTTTGATCAATTTCTTGATCAACTTGTGGAATTTACCGAGCTCGCTGATTTCATTCATCTACCAA
TTCGTACCTACAGCGACGGCATGAAAACTCGTCTTCAATTTGGCTTGTTAACTGCTTTTCGCCATGAGGCCTTGGCCCTC
GATGAGGGTATTGGAGCAGGGGATCAGTGGTTCCTTTCTCGTGCTCGTCAGCGATTGAGTGAATTTCTAGGTCAGGTGGG
CACGTTGATTTTGGCGTCTCACTCGGTGGAGTTGTTGACTCAGTTTTGCAACAGGGGCTTGGTTCTTAATCATGGCCGGG
TGGTGTTCGATGGGCCTTTGCATGAGGCTTTGACTCATTACTCAAAAGGGGTGACAGCATCATGA

Upstream 100 bases:

>100_bases
CTAAATTTGTTGGATCAAGAAAGGTTTTCCTCTGACTAGTGCTGTCAACGATCCCCTGGTTCAACTTGAGCATGTTTGTC
TTGACATTCCTGTTGATGGA

Downstream 100 bases:

>100_bases
GAGTGCCTCGTGCTGAAGATCAGCTAGCTCTTATTGAAACTGCTCCTGCGCAAGGTTTTGGCTTTATTTTTGTGTTGCGC
ACGGCTCGGGCCTGGTGGTA

Product: hypothetical protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MGSRSLKSAFVGGLTGGLLNRSRTRGLTVQALSDLNFTIQKGDRVALLGHNGAGKSTLLRLLADVYAPSQGRIRRNGHIA
PLINKSFWVDTDLSGRHAARAQYLLSRNTIDGFDQFLDQLVEFTELADFIHLPIRTYSDGMKTRLQFGLLTAFRHEALAL
DEGIGAGDQWFLSRARQRLSEFLGQVGTLILASHSVELLTQFCNRGLVLNHGRVVFDGPLHEALTHYSKGVTAS

Sequences:

>Translated_234_residues
MGSRSLKSAFVGGLTGGLLNRSRTRGLTVQALSDLNFTIQKGDRVALLGHNGAGKSTLLRLLADVYAPSQGRIRRNGHIA
PLINKSFWVDTDLSGRHAARAQYLLSRNTIDGFDQFLDQLVEFTELADFIHLPIRTYSDGMKTRLQFGLLTAFRHEALAL
DEGIGAGDQWFLSRARQRLSEFLGQVGTLILASHSVELLTQFCNRGLVLNHGRVVFDGPLHEALTHYSKGVTAS
>Mature_233_residues
GSRSLKSAFVGGLTGGLLNRSRTRGLTVQALSDLNFTIQKGDRVALLGHNGAGKSTLLRLLADVYAPSQGRIRRNGHIAP
LINKSFWVDTDLSGRHAARAQYLLSRNTIDGFDQFLDQLVEFTELADFIHLPIRTYSDGMKTRLQFGLLTAFRHEALALD
EGIGAGDQWFLSRARQRLSEFLGQVGTLILASHSVELLTQFCNRGLVLNHGRVVFDGPLHEALTHYSKGVTAS

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI87081791, Length=241, Percent_Identity=27.8008298755187, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081782, Length=222, Percent_Identity=28.8288288288288, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1787547, Length=239, Percent_Identity=25.1046025104602, Blast_Score=60, Evalue=1e-10,
Organism=Drosophila melanogaster, GI221500365, Length=190, Percent_Identity=27.8947368421053, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007184, Length=190, Percent_Identity=27.8947368421053, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25729; Mature: 25598

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSRSLKSAFVGGLTGGLLNRSRTRGLTVQALSDLNFTIQKGDRVALLGHNGAGKSTLLR
CCCHHHHHHHHHHHHHHHHCCHHCCCEEEEECCCCCEEEECCCEEEEEECCCCCHHHHHH
LLADVYAPSQGRIRRNGHIAPLINKSFWVDTDLSGRHAARAQYLLSRNTIDGFDQFLDQL
HHHHHHCCCCCCEECCCCEEEEECCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
VEFTELADFIHLPIRTYSDGMKTRLQFGLLTAFRHEALALDEGIGAGDQWFLSRARQRLS
HHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EFLGQVGTLILASHSVELLTQFCNRGLVLNHGRVVFDGPLHEALTHYSKGVTAS
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GSRSLKSAFVGGLTGGLLNRSRTRGLTVQALSDLNFTIQKGDRVALLGHNGAGKSTLLR
CCHHHHHHHHHHHHHHHHCCHHCCCEEEEECCCCCEEEECCCEEEEEECCCCCHHHHHH
LLADVYAPSQGRIRRNGHIAPLINKSFWVDTDLSGRHAARAQYLLSRNTIDGFDQFLDQL
HHHHHHCCCCCCEECCCCEEEEECCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
VEFTELADFIHLPIRTYSDGMKTRLQFGLLTAFRHEALALDEGIGAGDQWFLSRARQRLS
HHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EFLGQVGTLILASHSVELLTQFCNRGLVLNHGRVVFDGPLHEALTHYSKGVTAS
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]