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The map label for this gene is rfbB [H]

Identifier: 124021831

GI number: 124021831

Start: 118285

End: 119379

Strand: Direct

Name: rfbB [H]

Synonym: P9303_01181

Alternate gene names: 124021831

Gene position: 118285-119379 (Clockwise)

Preceding gene: 124021819

Following gene: 124021840

Centisome position: 4.41

GC content: 39.82

Gene sequence:

>1095_bases
ATGAGCACTGAAATCCCAGAATACTTCTCGAGGGTTCTTGTTACAGGAGGAGCTGGTTTTATAGGCGGAGCCCTCATAAA
ACGGTTGCTTAAGGAATCAAATATGATTATTTTTAACCTTGATAAATTCTCTTACGCTAGCGACCACACCTCCATCTATC
ATACCTTAGATAAACTTGACAAGTTAGCTTCTCAACGTTATAAGCCACTGCAGGTAGACTTAAGTGATCCGCTGTCCACT
CAAGCAGCCGTTTGTGAAGCTGACCCTGATCTTGTCTTTCATTTAGCAGCTGAGAGCCATGTAGACAGGTCTATCAATAA
CCCCCGCATTTTTTTCGAAAGTAATGTTGAAGGCACATTCAACTTATTGGAGTCATTACGCTCCCATTATGCTGAGCTTT
CAAAAGAACGACGAGCTCGTTTTCGCCTCCTTCACATCAGTACTGATGAGGTTTTTGGATCATTAGGTGATCAGGGCTTT
TTCTGTGAAACTTCGCCTTACCAACCACGCAGTCCTTATTCTGCTACCAAGGCTGCAAGTGACCATCTAGTTCAAGCTTG
GATTCACACATATGGTCTACCTGCCATAATCACAAATTGTACCAATAATTATGGACCTTATCAATTCCCTGAAAAGTTGA
TACCACTCGTCATTTTAAAAATGATTAATAATGAAGACATTCCAATTTATGGAAACGGTAAAAATGTTCGTGATTGGTTA
TTTGTCGAGGACCATATAGATGCACTTCTTCTCGTTGCCAGCAATGGCTTAATAGGTAATCGTTATTGCATAGGTGGTAC
TTCCGAACGAACAAATAAGCAGGTAGTAGAGTCAATAAGTATAATTATGGATAAATTACATCCTATCGGAGTACCTCATA
TACAAAAGATTAAATATGTCGATGATCGACCTGGCCATGATTTAAGGTACGCTATGGATATTAATCATATTAAAACTCAG
CTCGGGTGGAAACCTACCTTTTTATTCAATGTTGGCTTGGAAATTACTGTGAAATGGTACCTAGATCACTTGGCTTGGGT
CAAGCATGTAGCTAAAACATCTTCCTATCAAGGTGAGCGTCTAGGTCTTATCTAA

Upstream 100 bases:

>100_bases
AGTTACGTGTACTATACACTATAGGTGCTTAATTTTTTTTCTGAAACTAGTTCCTTTTACACTTTCACACTACTATACCC
ATTTTGCATATCTAAAAAAA

Downstream 100 bases:

>100_bases
ATAAGTCACTTTTATCTCCACTTATTTCTAGACTTTAATTTATCTCAGACTATATCCTTGTTAATTAACCCTAATTGCTC
CATTTATTTTTTCATTCTAT

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSTEIPEYFSRVLVTGGAGFIGGALIKRLLKESNMIIFNLDKFSYASDHTSIYHTLDKLDKLASQRYKPLQVDLSDPLST
QAAVCEADPDLVFHLAAESHVDRSINNPRIFFESNVEGTFNLLESLRSHYAELSKERRARFRLLHISTDEVFGSLGDQGF
FCETSPYQPRSPYSATKAASDHLVQAWIHTYGLPAIITNCTNNYGPYQFPEKLIPLVILKMINNEDIPIYGNGKNVRDWL
FVEDHIDALLLVASNGLIGNRYCIGGTSERTNKQVVESISIIMDKLHPIGVPHIQKIKYVDDRPGHDLRYAMDINHIKTQ
LGWKPTFLFNVGLEITVKWYLDHLAWVKHVAKTSSYQGERLGLI

Sequences:

>Translated_364_residues
MSTEIPEYFSRVLVTGGAGFIGGALIKRLLKESNMIIFNLDKFSYASDHTSIYHTLDKLDKLASQRYKPLQVDLSDPLST
QAAVCEADPDLVFHLAAESHVDRSINNPRIFFESNVEGTFNLLESLRSHYAELSKERRARFRLLHISTDEVFGSLGDQGF
FCETSPYQPRSPYSATKAASDHLVQAWIHTYGLPAIITNCTNNYGPYQFPEKLIPLVILKMINNEDIPIYGNGKNVRDWL
FVEDHIDALLLVASNGLIGNRYCIGGTSERTNKQVVESISIIMDKLHPIGVPHIQKIKYVDDRPGHDLRYAMDINHIKTQ
LGWKPTFLFNVGLEITVKWYLDHLAWVKHVAKTSSYQGERLGLI
>Mature_363_residues
STEIPEYFSRVLVTGGAGFIGGALIKRLLKESNMIIFNLDKFSYASDHTSIYHTLDKLDKLASQRYKPLQVDLSDPLSTQ
AAVCEADPDLVFHLAAESHVDRSINNPRIFFESNVEGTFNLLESLRSHYAELSKERRARFRLLHISTDEVFGSLGDQGFF
CETSPYQPRSPYSATKAASDHLVQAWIHTYGLPAIITNCTNNYGPYQFPEKLIPLVILKMINNEDIPIYGNGKNVRDWLF
VEDHIDALLLVASNGLIGNRYCIGGTSERTNKQVVESISIIMDKLHPIGVPHIQKIKYVDDRPGHDLRYAMDINHIKTQL
GWKPTFLFNVGLEITVKWYLDHLAWVKHVAKTSSYQGERLGLI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=349, Percent_Identity=34.9570200573066, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI42516563, Length=342, Percent_Identity=21.9298245614035, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI48994969, Length=358, Percent_Identity=49.7206703910614, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=46.6292134831461, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1788366, Length=179, Percent_Identity=30.7262569832402, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=33.4319526627219, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI115532424, Length=362, Percent_Identity=31.4917127071823, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=22.0588235294118, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356223, Length=343, Percent_Identity=26.530612244898, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41225; Mature: 41093

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIPEYFSRVLVTGGAGFIGGALIKRLLKESNMIIFNLDKFSYASDHTSIYHTLDKLD
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHH
KLASQRYKPLQVDLSDPLSTQAAVCEADPDLVFHLAAESHVDRSINNPRIFFESNVEGTF
HHHHHCCCCEEEECCCCCCCCCEEECCCCCEEEEEEHHHHHHHCCCCCEEEEECCCCHHH
NLLESLRSHYAELSKERRARFRLLHISTDEVFGSLGDQGFFCETSPYQPRSPYSATKAAS
HHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHH
DHLVQAWIHTYGLPAIITNCTNNYGPYQFPEKLIPLVILKMINNEDIPIYGNGKNVRDWL
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEE
FVEDHIDALLLVASNGLIGNRYCIGGTSERTNKQVVESISIIMDKLHPIGVPHIQKIKYV
EEHHHHCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEC
DDRPGHDLRYAMDINHIKTQLGWKPTFLFNVGLEITVKWYLDHLAWVKHVAKTSSYQGER
CCCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCC
LGLI
CCCC
>Mature Secondary Structure 
STEIPEYFSRVLVTGGAGFIGGALIKRLLKESNMIIFNLDKFSYASDHTSIYHTLDKLD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHH
KLASQRYKPLQVDLSDPLSTQAAVCEADPDLVFHLAAESHVDRSINNPRIFFESNVEGTF
HHHHHCCCCEEEECCCCCCCCCEEECCCCCEEEEEEHHHHHHHCCCCCEEEEECCCCHHH
NLLESLRSHYAELSKERRARFRLLHISTDEVFGSLGDQGFFCETSPYQPRSPYSATKAAS
HHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHH
DHLVQAWIHTYGLPAIITNCTNNYGPYQFPEKLIPLVILKMINNEDIPIYGNGKNVRDWL
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEE
FVEDHIDALLLVASNGLIGNRYCIGGTSERTNKQVVESISIIMDKLHPIGVPHIQKIKYV
EEHHHHCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEC
DDRPGHDLRYAMDINHIKTQLGWKPTFLFNVGLEITVKWYLDHLAWVKHVAKTSSYQGER
CCCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCC
LGLI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]