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The map label for this gene is thiG [H]

Identifier: 124021768

GI number: 124021768

Start: 53333

End: 54253

Strand: Reverse

Name: thiG [H]

Synonym: P9303_00531

Alternate gene names: 124021768

Gene position: 54253-53333 (Counterclockwise)

Preceding gene: 124021770

Following gene: 124021767

Centisome position: 2.02

GC content: 56.03

Gene sequence:

>921_bases
ATGGAAAAACATGATGATGGGCACAAGCTAAAGCCAATCGGCTCCACCGGGACACTCCGCAATGACGTTCCAACAACCTC
AACAGCGCCAATGGTTCTGACGCACTCCTCACCTGACCTGCTCACAATCGGCAAACGCAACTTCCACAGCCGACTTTTTG
CAGGCACAGGGAAGTACCCAAGCCTAAAGGTGATGCAACAGAGTCTGCGCTCTTCAGGATGCGAAATGGTGACGGTGGCA
GTTCGCCGTGTACAGGCAATGACATCAGGCCACGCCGGCCTGATCGAAGCCATCGACTGGTCAAAAATATGGATGCTGCC
CAACACCGCTGGCTGTGCAACTGCAGAGGAAGCCATTCGTGTAGCCCGACTCGGCCGCGAACTCGCCAAACTGGCAGGCC
AGGAGGACAACAAATTCGTCAAGCTCGAAGTCATCCCCGACAGCCGCCACCTCCTACCTGACCCTTTCGGTACCCTTGAG
GCCGCTGAACAATTGGTGAAAGAAGGCTTCGAGGTTTTGCCATACATCAATGCCGATCCCCTCTTGGCTAAGCGCCTTGA
AGAGGTGGGCTGTGCGACGGTCATGCCACTGGGCTCACCGATTGGATCAGGTCAAGGTCTGAGGAATGCCGCCAACATCT
CCCTAATCATTGAAAACGCCCGAGTGCCAGTCGTTGTGGATGCTGGCATCGGTGTCCCCAGTGAAGCTGCACAGGCCCTG
GAAATGGGTGCCGACGCAGTGCTGGTGAACAGCGCAATTGCCTTAGCCGGTGATCCAATCACCATGGCCGAAGCAATGCG
TTGGGCGATTCAGGCCGGTAGGCAGGCTTACAGATCAGGTCGCCTACCCGAACGCACCGCAGCCTCGCCAAGCTCACCGA
CAACAGGGATTATTACGGAAGCCAAGACTAAACAATTATGA

Upstream 100 bases:

>100_bases
CACGACCAGCGGCACAGCCAATTGAGAGGAATCCAAGGGCATAGTGAGCTCGAAGGGAACCTCACTGTCAATCGAGAGAT
CCATAGCGAGCAGGATGGGC

Downstream 100 bases:

>100_bases
AGGTACGTAACGGAAAAGCTCCTGCAAGCAATCCTGTCCATAGGGTCCGCCGAGTTGTAAGTGGTACATGCAAGTCACCG
AGGAAGAGGGTGGTCGACTC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MEKHDDGHKLKPIGSTGTLRNDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYPSLKVMQQSLRSSGCEMVTVA
VRRVQAMTSGHAGLIEAIDWSKIWMLPNTAGCATAEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRHLLPDPFGTLE
AAEQLVKEGFEVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPSEAAQAL
EMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERTAASPSSPTTGIITEAKTKQL

Sequences:

>Translated_306_residues
MEKHDDGHKLKPIGSTGTLRNDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYPSLKVMQQSLRSSGCEMVTVA
VRRVQAMTSGHAGLIEAIDWSKIWMLPNTAGCATAEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRHLLPDPFGTLE
AAEQLVKEGFEVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPSEAAQAL
EMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERTAASPSSPTTGIITEAKTKQL
>Mature_306_residues
MEKHDDGHKLKPIGSTGTLRNDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYPSLKVMQQSLRSSGCEMVTVA
VRRVQAMTSGHAGLIEAIDWSKIWMLPNTAGCATAEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRHLLPDPFGTLE
AAEQLVKEGFEVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPSEAAQAL
EMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERTAASPSSPTTGIITEAKTKQL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=259, Percent_Identity=53.2818532818533, Blast_Score=260, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 32518; Mature: 32518

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHDDGHKLKPIGSTGTLRNDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYP
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHEECCCCCC
SLKVMQQSLRSSGCEMVTVAVRRVQAMTSGHAGLIEAIDWSKIWMLPNTAGCATAEEAIR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
VARLGRELAKLAGQEDNKFVKLEVIPDSRHLLPDPFGTLEAAEQLVKEGFEVLPYINADP
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCCCCCH
LLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPSEAAQAL
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHH
EMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERTAASPSSPTTGIITE
HHCCCEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEC
AKTKQL
CHHCCC
>Mature Secondary Structure
MEKHDDGHKLKPIGSTGTLRNDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYP
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHEECCCCCC
SLKVMQQSLRSSGCEMVTVAVRRVQAMTSGHAGLIEAIDWSKIWMLPNTAGCATAEEAIR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
VARLGRELAKLAGQEDNKFVKLEVIPDSRHLLPDPFGTLEAAEQLVKEGFEVLPYINADP
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCCCCCH
LLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPSEAAQAL
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHH
EMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERTAASPSSPTTGIITE
HHCCCEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEC
AKTKQL
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]