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The map label for this gene is dus2 [H]

Identifier: 124021729

GI number: 124021729

Start: 17882

End: 18886

Strand: Reverse

Name: dus2 [H]

Synonym: P9303_00141

Alternate gene names: 124021729

Gene position: 18886-17882 (Counterclockwise)

Preceding gene: 124021732

Following gene: 124021721

Centisome position: 0.7

GC content: 53.53

Gene sequence:

>1005_bases
ATGATTGTTCCTTTCACGCCACAGGTTGATGGTGCTTATCGGTTCAGCGTGGCTCCAATGCTCGACTGCACAGATCGACA
CTTCAGGGTACTAATGCGACAAATCAGTCGCCGGGCGTTGCTTTACACGGAAATGTTGGTTGCCCAAGCTCTGCATCACA
GCAACCGTCTTGATCATCTGCTCGATTTCGACATCATCGAGCATCCCCTGTCTCTACAAGTAGGGGGCGATGATCCAAAA
ATGCTTGCAGAAGCAGCGCGCCTGGCCGATGCCTGGGGCTACGACGAAATCAACCTCAACGTGGGATGTCCCAGCTCAAG
AGCAAAAGCAGGCAACTTCGGTGCCTGCCTAATGGCTAAACCTGATCAAGTCGCACGTTGTGTTGAAGCGATGGCGATGG
CGAGCCCTCTTCCAGTCACCGTGAAACACCGTCTAGGAATTGATGATTTCGATAGCGACGCTCTACTCATGACCTTTGTC
GACCGAGTGTCCCTCGCAGGAGCCACTCGCTTTACTGTGCATGCACGAAAAGCCTGGCTAGAAGGGCTTGACCCCAAACA
AAACCGCACGATTCCACCACTTCAACATCAACGAGTCACCCATCTCAAGCAACAACGCCCGCAGCTCACTATTGAAATCA
ATGGAGGACTAGAACACCCTGCCGACTGCCTAACAGCGCTGCAAACCTGTGATGGGGCAATGGTGGGGCGAGCAGCGTAT
GCGCATCCGCTCCGCTGGAAGAGCATGGATGAGCTGGTCTATGGAGAAGAACCACGCTCAATCAATGCTTCTCAAGTCAT
AGGAGGATTACTCCCTCATGCCGAAACCCACCTGAGCCGAGGTGGCCGGCTATGGGATCTTTGCCGACATCTTTTACAAC
TCGTTGAAGGGGTACCGGGCGCCAAATCCTGGAGGCGAGACCTTGGCATCAAGGCTCAAAAAGCCGATGCCGATCTAACA
GTGCTGCAAAAAGCAGCCCAGCAACTTGAAGATGCCGGGCTATAA

Upstream 100 bases:

>100_bases
GACGACTCAATAGCAAAGCAGTAATGGGCATAAGCGCAGCCAAAATCAATGACCTTCTTTCTAGGAAAGCGCAACACAGC
TATGAAGGCAGCATGAATCA

Downstream 100 bases:

>100_bases
GGACTTCACTCAGCCCTCCGCTCCCCCATATCCGCTGTAGCTGTCACCACCTTCACCCTCAGGTGCAGCTCCTCCCCTAC
GACGACGAGTGCGACCGTCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIVPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHLLDFDIIEHPLSLQVGGDDPK
MLAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDDFDSDALLMTFV
DRVSLAGATRFTVHARKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMVGRAAY
AHPLRWKSMDELVYGEEPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPGAKSWRRDLGIKAQKADADLT
VLQKAAQQLEDAGL

Sequences:

>Translated_334_residues
MIVPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHLLDFDIIEHPLSLQVGGDDPK
MLAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDDFDSDALLMTFV
DRVSLAGATRFTVHARKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMVGRAAY
AHPLRWKSMDELVYGEEPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPGAKSWRRDLGIKAQKADADLT
VLQKAAQQLEDAGL
>Mature_334_residues
MIVPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHLLDFDIIEHPLSLQVGGDDPK
MLAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDDFDSDALLMTFV
DRVSLAGATRFTVHARKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMVGRAAY
AHPLRWKSMDELVYGEEPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPGAKSWRRDLGIKAQKADADLT
VLQKAAQQLEDAGL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=247, Percent_Identity=27.9352226720648, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI40807366, Length=166, Percent_Identity=32.5301204819277, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=47.5757575757576, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788462, Length=266, Percent_Identity=27.8195488721804, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=298, Percent_Identity=26.510067114094, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=211, Percent_Identity=28.9099526066351, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36962; Mature: 36962

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHL
CCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LDFDIIEHPLSLQVGGDDPKMLAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAK
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEC
PDQVARCVEAMAMASPLPVTVKHRLGIDDFDSDALLMTFVDRVSLAGATRFTVHARKAWL
HHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
EGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMVGRAAY
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHH
AHPLRWKSMDELVYGEEPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCCC
AKSWRRDLGIKAQKADADLTVLQKAAQQLEDAGL
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHL
CCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LDFDIIEHPLSLQVGGDDPKMLAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAK
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEC
PDQVARCVEAMAMASPLPVTVKHRLGIDDFDSDALLMTFVDRVSLAGATRFTVHARKAWL
HHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
EGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMVGRAAY
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHH
AHPLRWKSMDELVYGEEPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCCC
AKSWRRDLGIKAQKADADLTVLQKAAQQLEDAGL
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]