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The map label for this gene is purU [H]

Identifier: 123969444

GI number: 123969444

Start: 1651395

End: 1652267

Strand: Reverse

Name: purU [H]

Synonym: A9601_19121

Alternate gene names: 123969444

Gene position: 1652267-1651395 (Counterclockwise)

Preceding gene: 123969446

Following gene: 123969441

Centisome position: 98.94

GC content: 30.81

Gene sequence:

>873_bases
TTGATAGTTAAAGATTTTTTGGAACATCCTTCAATTATATTCAAAATTGTTTGTCCTGATCGTCCTGGCCTTGTGAGTTT
ACTTACAAGTTGGATTTCAAACTACGGTGGCAACATAAAACATTCTGATCATCATACAGATCAAGATGCGGGTTTGTTCC
TTAGTCGAATTGAATGGAATAGTAAAAATGCATTTTTGAATAGAGATGAAATTTATAAAGAATTTGAAAAAATTGCAGAT
GAAGTAAATGGAAAATTTAATGTAAATTATTCTGATGAAATTCCAAATGTTGCAATTTTTGTGAGTAAACAAAATCATTG
CTTGATTGATTTACTTTGGCGAGTAAGAAATGGTGAATTGAAAATGCAAGTGCCGGTAATAATTTCGAATCATTCTGACC
TTGAAAATATTGCAAACGACTTTAATGCAAAATTTGTCTATGTTGATACCTTTAATATTGACAAATCTGTTGTTGAAGAT
CAATTTTTAAATTTATTAAAAGAATATGAAATTGATCTTGTTGTGTTAGCCAAATATATGCAAATTTTGAGTGACTCTTT
TTTAAAAAAGTTCTCTTCAATAATCAATATTCATCATTCTTTCTTACCTGCATTTAAGGGCGGTCAACCATATCATCGAG
CATGGAAGAGAGGTGTTAAATTAATCGGTGCCACAGCTCACTATGTTACTGAAGATCTTGATGAAGGTCCGATCATAGAG
CAATGCACAGTTAATGTAAGTCATAGGGATGAAGTTGATGATTTGATTAGAAAAGGAAGAGATATTGAAAGAATTGCTCT
AGCAAGAGCAGTTAGATTACATCTGAATCATCAAGTAATTGTTTATAATAGTAAAACTGCTGTTTTTGATTGA

Upstream 100 bases:

>100_bases
AATTTTCCTATAATCCCTGATCCAATTACAAGTATGCGTGCTTTTTGAAAATTTTCTTTTAAGCTTTTCATTGATATATT
AGATATATTGCTTATTTGAC

Downstream 100 bases:

>100_bases
AGATAGTTCCTTAATCTTCTAACTCTATTTGTATTTGTCTTTCATAAATTGATTCTTCATTAAAAGCTCTTGCTATCAAG
AAACTGGCAATGCAGCTGAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIVKDFLEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNAFLNRDEIYKEFEKIAD
EVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMQVPVIISNHSDLENIANDFNAKFVYVDTFNIDKSVVED
QFLNLLKEYEIDLVVLAKYMQILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIE
QCTVNVSHRDEVDDLIRKGRDIERIALARAVRLHLNHQVIVYNSKTAVFD

Sequences:

>Translated_290_residues
MIVKDFLEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNAFLNRDEIYKEFEKIAD
EVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMQVPVIISNHSDLENIANDFNAKFVYVDTFNIDKSVVED
QFLNLLKEYEIDLVVLAKYMQILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIE
QCTVNVSHRDEVDDLIRKGRDIERIALARAVRLHLNHQVIVYNSKTAVFD
>Mature_290_residues
MIVKDFLEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWNSKNAFLNRDEIYKEFEKIAD
EVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGELKMQVPVIISNHSDLENIANDFNAKFVYVDTFNIDKSVVED
QFLNLLKEYEIDLVVLAKYMQILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIE
QCTVNVSHRDEVDDLIRKGRDIERIALARAVRLHLNHQVIVYNSKTAVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=197, Percent_Identity=32.994923857868, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209869995, Length=197, Percent_Identity=32.994923857868, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209869993, Length=197, Percent_Identity=32.994923857868, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=35.6115107913669, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1788846, Length=167, Percent_Identity=27.5449101796407, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=165, Percent_Identity=33.9393939393939, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24582400, Length=128, Percent_Identity=31.25, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33408; Mature: 33408

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKDFLEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWN
CCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEC
SKNAFLNRDEIYKEFEKIADEVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGEL
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCE
KMQVPVIISNHSDLENIANDFNAKFVYVDTFNIDKSVVEDQFLNLLKEYEIDLVVLAKYM
EEEEEEEEECCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
QILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHEECHHHHHHHHCCCCCCCCH
QCTVNVSHRDEVDDLIRKGRDIERIALARAVRLHLNHQVIVYNSKTAVFD
HEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIVKDFLEHPSIIFKIVCPDRPGLVSLLTSWISNYGGNIKHSDHHTDQDAGLFLSRIEWN
CCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEC
SKNAFLNRDEIYKEFEKIADEVNGKFNVNYSDEIPNVAIFVSKQNHCLIDLLWRVRNGEL
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCE
KMQVPVIISNHSDLENIANDFNAKFVYVDTFNIDKSVVEDQFLNLLKEYEIDLVVLAKYM
EEEEEEEEECCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
QILSDSFLKKFSSIINIHHSFLPAFKGGQPYHRAWKRGVKLIGATAHYVTEDLDEGPIIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHEECHHHHHHHHCCCCCCCCH
QCTVNVSHRDEVDDLIRKGRDIERIALARAVRLHLNHQVIVYNSKTAVFD
HEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]