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The map label for this gene is trpE [H]

Identifier: 123969316

GI number: 123969316

Start: 1517744

End: 1519264

Strand: Reverse

Name: trpE [H]

Synonym: A9601_17841

Alternate gene names: 123969316

Gene position: 1519264-1517744 (Counterclockwise)

Preceding gene: 123969317

Following gene: 123969315

Centisome position: 90.98

GC content: 33.86

Gene sequence:

>1521_bases
ATGATCAGCTCACAAAAAGATAGTTTTTTAAAGGCTTACAAAGAAGGTAAAAACTTTATACCTATAGTTGAAACTTGGCC
AGCAGATTTAGAGACTCCATTATCGACTTGGTTAAAATTATCTTCAAAAGATTCCCATGGTGTTTTTCTTGAATCTGTTG
AGGGTGGCGAGAATTTGGGTAGGTGGAGTATTGTTGCTACTCAACCTCTTTGGGAAGCCGTTTGTTATGGAGAAGAAATA
ATTAAAACTTGGAATAATGGCAAAACTGAAACACATAAAGGTGATCCTTTTGATATTTTGAGAAGTTGGACAAACGAATA
CAAGTCAACCACGCTTGATGAATTACCCTCAATTGGACAGTTATATGGCTCTTGGGGTTATGAATTAATAAATCGAATAG
AACCAAGCGTTCCAATAAATGAAAAATTAGAAAACAATATCCCTTATGGTTCCTGGATGTTTTTTGATCAGATAGTTGTT
TTTGATCAAATAAAAAGATGTATTACTGCAGTGGTTTATGCAGATACAACTTCTACAAAAGAGTGCGAAATTGAACTGTT
GTACCTAAACTCAATTTCTAGAATTAAGAAAACTAGAAATTTAATGAGAGTTCCTCTAAAAGAAAATGAGTTTTTAGATT
GGAATGAAAATGAGAATTTGAATTTAGATCTAGAAAGTAATTGGGAGAAAAAAGATTTTGAGGATGCAGTTCTCTCTGCA
AAAGAATATATAAGAAAGGGAGATATCTTCCAAATAGTTATTAGTCAGAGATTCCAAACTCAAGTCAATAATGATCCCTT
TAATTTATATAGAAGTCTGAGAATGGTTAATCCATCTCCATACATGTCATTTTTTGATTTTGGCTCATGGTATCTGATAG
GTTCAAGTCCTGAAGTCATGGTTAAAGCAGAAAAAAATAAAAATAGTCAGATCGTTGCAAGCTTAAGACCAATAGCTGGC
ACTAGACCTAGAGGTATTGATAATCAGCAAGACTTGGAATTAGAAAAGGAATTATTAAAAGATCCAAAAGAGATAGCTGA
GCATGTAATGCTAATTGATCTTGGGAGAAATGATCTTGGAAGAGTTTGTGAAATTGGTACTGTCAAGGTCAAGGATTTAA
TGGTTATTGAGAAATATTCACATGTTATGCATATAGTCAGTCAAGTTGAGGGAATCTTAAAAAATAATGCTGATGTATGG
GATTTGCTCAAAGCATCCTTTCCCGCTGGGACAGTAACTGGCGCTCCAAAAATAAGAGCTATGCAATTGATTAAGCACTT
TGAAAAAGATGCTAGAGGACCTTATGCAGGTGTATACGGATCTATTGATATTAATGGCGCATTAAATACAGCAATTACAA
TAAGAACTATGATAGTAAAACCCTCAATAGATGGGAAATATGATGTTTCAGTGCAAGCAGGAGCTGGAATAGTTGCTGAT
TCTTTTCCTGAAAATGAATATCAAGAGACGATAAATAAAGCAAAGGGAATACTAAAAGCACTAGCCTGTTTGGATAAATA
A

Upstream 100 bases:

>100_bases
CAGCTGGTTTGAAATTTACAACTAAGAATACTTTTGATTAACTTCTTTAAAGTTGATATAAAAGAAGAAAATAATTATAA
TTTGGGCTGACATTATTAAT

Downstream 100 bases:

>100_bases
ATGAGTCAAAATAATCTTTATAAGGGTTTTGAGGTGGAACTTTTTACAGGCTCTTTAAATTCTCATATTGGTGTTTCGGA
TGAAATTGAGAAAATATTTC

Product: anthranilate synthase component I/chorismate-binding protein

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MISSQKDSFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLGRWSIVATQPLWEAVCYGEEI
IKTWNNGKTETHKGDPFDILRSWTNEYKSTTLDELPSIGQLYGSWGYELINRIEPSVPINEKLENNIPYGSWMFFDQIVV
FDQIKRCITAVVYADTTSTKECEIELLYLNSISRIKKTRNLMRVPLKENEFLDWNENENLNLDLESNWEKKDFEDAVLSA
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVKVKDLMVIEKYSHVMHIVSQVEGILKNNADVW
DLLKASFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSIDGKYDVSVQAGAGIVAD
SFPENEYQETINKAKGILKALACLDK

Sequences:

>Translated_506_residues
MISSQKDSFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLGRWSIVATQPLWEAVCYGEEI
IKTWNNGKTETHKGDPFDILRSWTNEYKSTTLDELPSIGQLYGSWGYELINRIEPSVPINEKLENNIPYGSWMFFDQIVV
FDQIKRCITAVVYADTTSTKECEIELLYLNSISRIKKTRNLMRVPLKENEFLDWNENENLNLDLESNWEKKDFEDAVLSA
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVKVKDLMVIEKYSHVMHIVSQVEGILKNNADVW
DLLKASFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSIDGKYDVSVQAGAGIVAD
SFPENEYQETINKAKGILKALACLDK
>Mature_506_residues
MISSQKDSFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLGRWSIVATQPLWEAVCYGEEI
IKTWNNGKTETHKGDPFDILRSWTNEYKSTTLDELPSIGQLYGSWGYELINRIEPSVPINEKLENNIPYGSWMFFDQIVV
FDQIKRCITAVVYADTTSTKECEIELLYLNSISRIKKTRNLMRVPLKENEFLDWNENENLNLDLESNWEKKDFEDAVLSA
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVMVKAEKNKNSQIVASLRPIAG
TRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLGRVCEIGTVKVKDLMVIEKYSHVMHIVSQVEGILKNNADVW
DLLKASFPAGTVTGAPKIRAMQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSIDGKYDVSVQAGAGIVAD
SFPENEYQETINKAKGILKALACLDK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=388, Percent_Identity=36.340206185567, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788114, Length=465, Percent_Identity=30.752688172043, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082077, Length=289, Percent_Identity=25.6055363321799, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786809, Length=292, Percent_Identity=25.3424657534247, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=490, Percent_Identity=35.3061224489796, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324361, Length=416, Percent_Identity=27.4038461538462, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57388; Mature: 57388

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSQKDSFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLG
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHEECCCCCCCEEEEECCCCCCCC
RWSIVATQPLWEAVCYGEEIIKTWNNGKTETHKGDPFDILRSWTNEYKSTTLDELPSIGQ
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCHHHH
LYGSWGYELINRIEPSVPINEKLENNIPYGSWMFFDQIVVFDQIKRCITAVVYADTTSTK
HHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCC
ECEIELLYLNSISRIKKTRNLMRVPLKENEFLDWNENENLNLDLESNWEKKDFEDAVLSA
CEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVM
HHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCEE
VKAEKNKNSQIVASLRPIAGTRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCC
RVCEIGTVKVKDLMVIEKYSHVMHIVSQVEGILKNNADVWDLLKASFPAGTVTGAPKIRA
CHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHH
MQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSIDGKYDVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEC
SFPENEYQETINKAKGILKALACLDK
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISSQKDSFLKAYKEGKNFIPIVETWPADLETPLSTWLKLSSKDSHGVFLESVEGGENLG
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHEECCCCCCCEEEEECCCCCCCC
RWSIVATQPLWEAVCYGEEIIKTWNNGKTETHKGDPFDILRSWTNEYKSTTLDELPSIGQ
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCHHHH
LYGSWGYELINRIEPSVPINEKLENNIPYGSWMFFDQIVVFDQIKRCITAVVYADTTSTK
HHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCC
ECEIELLYLNSISRIKKTRNLMRVPLKENEFLDWNENENLNLDLESNWEKKDFEDAVLSA
CEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
KEYIRKGDIFQIVISQRFQTQVNNDPFNLYRSLRMVNPSPYMSFFDFGSWYLIGSSPEVM
HHHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCEE
VKAEKNKNSQIVASLRPIAGTRPRGIDNQQDLELEKELLKDPKEIAEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCC
RVCEIGTVKVKDLMVIEKYSHVMHIVSQVEGILKNNADVWDLLKASFPAGTVTGAPKIRA
CHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHH
MQLIKHFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSIDGKYDVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEC
SFPENEYQETINKAKGILKALACLDK
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 3141423 [H]