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The map label for this gene is truA [H]

Identifier: 123969273

GI number: 123969273

Start: 1484755

End: 1485561

Strand: Reverse

Name: truA [H]

Synonym: A9601_17411

Alternate gene names: 123969273

Gene position: 1485561-1484755 (Counterclockwise)

Preceding gene: 123969274

Following gene: 123969272

Centisome position: 88.96

GC content: 30.24

Gene sequence:

>807_bases
TTGAAAAGGGTAGCTTTACTAGTCCAATATGATGGATCTCATTATTCAGGCTGGCAAAAACAAAAAAATGCAAATACTAT
TCAAGAAATTTTAGATAAAGCTCTCTTAAAGATTACAAATCATACAGTTAAGACTTTTGCTGCAGGTAGGACTGATGCCG
GGGTGCATGCATCAGGTCAAGTTATACATTTTGATATTGATTGTGTTATTCCAGGAAATAGTTATTCTGATGTCTTAAAT
AGTCTTTTACCCTCAACAATTAGGATTTTGGAATCAGTTGAGGTTAAAGATAGTTGGCATGCATGCTATTCAGCAAGTTA
TAGACATTATCGGTATGTCATTAATAACAGTAAATTCCCTAACTTGTTTATTAACAATTGGTCATGGCATAGATATCAAA
AAGTATTAGATGAAGTTTTAATGTTAAATGCATCCAAGAAAATGGAAGGTGAGCATGATTTTTTTGCTTTTCAGAAAAGT
GGAAGTAACAGGCAAAATTCTATTACAAAAATAAAAAATATAGATGTTAAAAGAGTAGAGGATTTGATTTTAGTTGATAT
TAAAGCTACTGGTTTTCTATATGGAATGGTTCGTTTAATTGTTGGTCAACTAGTTTTAGTCGGAGAAAAAAAAATATCGC
CAGAAATTTTTACAGATAGATGGGTAAACAAAAAGAAAAATGATGTAAAGGAATCTGCTCCAGCCAAGGGGTTATGTTTT
GTAAATGCTGTTTATGAAGAAAATGTTTTTAAAAAGATTAAAAACAACGATTTTTTTCCTATATTTTTAATTGAAGGTTT
TTCTTAA

Upstream 100 bases:

>100_bases
ATAGTCAGAACCGTATCAAGAAAAGGAGATAACGCTCAGATGGCCATAATAGAGCTTGTTTAATTAAGTTATTAAGACCG
CTTTTACTAGAAAATAACTT

Downstream 100 bases:

>100_bases
AATAAATTTTAATTGAGCAAATTTTTTGTGAAAATCATTGGAAAATGTTGTTTATTATTCCTCTAATAAGGTAGAATTTA
GAACTAACTTTAAATTTATT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRVALLVQYDGSHYSGWQKQKNANTIQEILDKALLKITNHTVKTFAAGRTDAGVHASGQVIHFDIDCVIPGNSYSDVLN
SLLPSTIRILESVEVKDSWHACYSASYRHYRYVINNSKFPNLFINNWSWHRYQKVLDEVLMLNASKKMEGEHDFFAFQKS
GSNRQNSITKIKNIDVKRVEDLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDRWVNKKKNDVKESAPAKGLCF
VNAVYEENVFKKIKNNDFFPIFLIEGFS

Sequences:

>Translated_268_residues
MKRVALLVQYDGSHYSGWQKQKNANTIQEILDKALLKITNHTVKTFAAGRTDAGVHASGQVIHFDIDCVIPGNSYSDVLN
SLLPSTIRILESVEVKDSWHACYSASYRHYRYVINNSKFPNLFINNWSWHRYQKVLDEVLMLNASKKMEGEHDFFAFQKS
GSNRQNSITKIKNIDVKRVEDLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDRWVNKKKNDVKESAPAKGLCF
VNAVYEENVFKKIKNNDFFPIFLIEGFS
>Mature_268_residues
MKRVALLVQYDGSHYSGWQKQKNANTIQEILDKALLKITNHTVKTFAAGRTDAGVHASGQVIHFDIDCVIPGNSYSDVLN
SLLPSTIRILESVEVKDSWHACYSASYRHYRYVINNSKFPNLFINNWSWHRYQKVLDEVLMLNASKKMEGEHDFFAFQKS
GSNRQNSITKIKNIDVKRVEDLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDRWVNKKKNDVKESAPAKGLCF
VNAVYEENVFKKIKNNDFFPIFLIEGFS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI199559785, Length=267, Percent_Identity=31.0861423220974, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1788657, Length=263, Percent_Identity=33.4600760456274, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321112, Length=240, Percent_Identity=29.5833333333333, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30686; Mature: 30686

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVALLVQYDGSHYSGWQKQKNANTIQEILDKALLKITNHTVKTFAAGRTDAGVHASGQ
CCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VIHFDIDCVIPGNSYSDVLNSLLPSTIRILESVEVKDSWHACYSASYRHYRYVINNSKFP
EEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCC
NLFINNWSWHRYQKVLDEVLMLNASKKMEGEHDFFAFQKSGSNRQNSITKIKNIDVKRVE
EEEEECCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHC
DLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDRWVNKKKNDVKESAPAKGLCF
EEEEEEEEHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEE
VNAVYEENVFKKIKNNDFFPIFLIEGFS
EEHHHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MKRVALLVQYDGSHYSGWQKQKNANTIQEILDKALLKITNHTVKTFAAGRTDAGVHASGQ
CCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VIHFDIDCVIPGNSYSDVLNSLLPSTIRILESVEVKDSWHACYSASYRHYRYVINNSKFP
EEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCC
NLFINNWSWHRYQKVLDEVLMLNASKKMEGEHDFFAFQKSGSNRQNSITKIKNIDVKRVE
EEEEECCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHC
DLILVDIKATGFLYGMVRLIVGQLVLVGEKKISPEIFTDRWVNKKKNDVKESAPAKGLCF
EEEEEEEEHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEE
VNAVYEENVFKKIKNNDFFPIFLIEGFS
EEHHHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA