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The map label for this gene is cobJ [H]

Identifier: 123969261

GI number: 123969261

Start: 1474310

End: 1476112

Strand: Direct

Name: cobJ [H]

Synonym: A9601_17291

Alternate gene names: 123969261

Gene position: 1474310-1476112 (Clockwise)

Preceding gene: 123969257

Following gene: 123969262

Centisome position: 88.29

GC content: 30.39

Gene sequence:

>1803_bases
TTGAAAGGCATTGCGATAGGTCTATCTAATAATTCAAAAGAAATTTTAAAAAGACTTAAAAAAACTGAATTTGTCAAAGA
CATCTATATTGCAGGTTCTTCTAAAGATGCAGGAAAAGTAAATGAACTTATTCAAGTACAAAAACCAAAAAAAATTTTAC
TTAAAAAATGGTCGGAGATAGATTTAATAATTTTTATAGGCTCAATTGCTGCATCAATACGCATTATAAATTCTTTTTTA
ACCTCTAAAGATCAAGATCCAAGTGTAATCGTTATAGATAAAAAATGCTCCAAGATAGTTCCTTTAATTGGCTTACATCA
TTCAAATACGCAAAATATTGCGTTTCAAATTGCTAATTTATTTGGTGGCGAAATAATAGAAACTAATAATTCCAATGATC
AAAGCTTTTTAAATCTTGATGCATTTGGGTATCAATGGGGTTGGAAAAGATCTGGCAAAATAAAGGATTGGTCAAAACTA
GTAATTAAACAAGCTAAGAACGAAGAAATTTTTTGCAAACAATTATCTGGTAATAGCTTATGGAAAACTTCAGAATCAGC
TGAGATTATTAATCAAATTAATGAAAAAGAAACTGAAAAACCAGATTCAACATTTCATGTAAGTATATTCAAAAAGCATT
TAACTACTTGGCATCCTCCCGTATTATGGGTTGGCATTGGATGTGAAAGAAATACAAGCAAAGAATTAATAGCAAATTCT
TTTAATAATTTTTTGGAGTCAGGAAATTTCTCTCAGCAATCAATTGCGGGATTTGCAACTATTGATATTAAAAAAGATGA
GAAAGGAATTTTAGAACTTTCCAAAGAAAAAAACTTGCCTATAAAGTTTTTTAGTAAAGAAGATCTTTCAACAATAATTG
TTCCAAATCCATCAACCGTAGTTCAGAAGGAAATTGGCACACCTTCCGTAGCAGAAGCCTCTTGCTTACTGGCAGCTGGA
GAAGAATCAAAATTATTAAAAGAAAAAAGAATTTTTAAAAATCAATCTGGGGCAGTAACTATTGCTGTAGCAGAATCAAA
AAATCAATATAATCCAACCCATGGCGAAATACATATTATTGGAAGTGGGCCTGGTGATATCTCCTTTTTAACTAGTAATG
CAAAAAAAGCACTTTCAAGATGTACTGTTTGGATCGGATACAAAATGTATTTAGATTTAATTAAGCCCTTAAAAAGGAAT
GATCAAGTTTTAATTGAAAGTAAACTTACTGAAGAAAAAGATAGATGCAGTAAAGCAATTAAACTAGCCGAGGAAGGAAT
AAAAGTGGCTTTAATTTCTTCAGGTGAATCTGGATTTTATGGCATGGCTGGTTTACTTTTAGAATTACTTCAAAAAATCA
AAATAGAATATAGACCTTACTTTGAAGTTCATCCAGGTATAAGTAGTGTTCAATTGGCTGCTGCGATTAGTGGAGCACCA
TTAATGAATGACTTTTGTTCAATAAGCTTAAGCGATAAATTAACTCCATGGTCTTTAATAGAAAAAAGAATTGCAGGAGC
TCTTATGGGTGACTTTGTAATAGCTTTATTTAATCCACAATCCATTGAAAGAAATTGGCAGCTAAAAATTGTAATAGATA
TATGTTTAAAATCTAGGCTTGGGAATACTCCAGTTTTAATAGCTAGACAAGTAGGGAGAGAAAATCAATCCAAAAAATTT
TTTACTTTAAACACTATCCCTTTTAAAGAGATTGATATGTTATCAATCATTATTATTGGCAATTCTCAAACAACCTTAGT
TGATGAAATATTTCTCACTCCCAGAGGATACTTAAAAAATTAA

Upstream 100 bases:

>100_bases
TTTTATATAAATTTCTTAAATTTTTTTTTATATTTCAAAGAAAATTTTTTAAATCCAGCGACAGGATATAATTTCAAAGT
TACTACCAATAGTTTCTAAT

Downstream 100 bases:

>100_bases
GTTTCAATAATCCATAATTTAATAATATAGGAAGTTTTTCTTTGCTTTTTTTTTATAAGAAGACTAATCTTTTTATAATA
ATAGTTAAATAATTAATTGA

Product: bifunctional cbiH protein and precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDAGKVNELIQVQKPKKILLKKWSEIDLIIFIGSIAASIRIINSFL
TSKDQDPSVIVIDKKCSKIVPLIGLHHSNTQNIAFQIANLFGGEIIETNNSNDQSFLNLDAFGYQWGWKRSGKIKDWSKL
VIKQAKNEEIFCKQLSGNSLWKTSESAEIINQINEKETEKPDSTFHVSIFKKHLTTWHPPVLWVGIGCERNTSKELIANS
FNNFLESGNFSQQSIAGFATIDIKKDEKGILELSKEKNLPIKFFSKEDLSTIIVPNPSTVVQKEIGTPSVAEASCLLAAG
EESKLLKEKRIFKNQSGAVTIAVAESKNQYNPTHGEIHIIGSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRN
DQVLIESKLTEEKDRCSKAIKLAEEGIKVALISSGESGFYGMAGLLLELLQKIKIEYRPYFEVHPGISSVQLAAAISGAP
LMNDFCSISLSDKLTPWSLIEKRIAGALMGDFVIALFNPQSIERNWQLKIVIDICLKSRLGNTPVLIARQVGRENQSKKF
FTLNTIPFKEIDMLSIIIIGNSQTTLVDEIFLTPRGYLKN

Sequences:

>Translated_600_residues
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDAGKVNELIQVQKPKKILLKKWSEIDLIIFIGSIAASIRIINSFL
TSKDQDPSVIVIDKKCSKIVPLIGLHHSNTQNIAFQIANLFGGEIIETNNSNDQSFLNLDAFGYQWGWKRSGKIKDWSKL
VIKQAKNEEIFCKQLSGNSLWKTSESAEIINQINEKETEKPDSTFHVSIFKKHLTTWHPPVLWVGIGCERNTSKELIANS
FNNFLESGNFSQQSIAGFATIDIKKDEKGILELSKEKNLPIKFFSKEDLSTIIVPNPSTVVQKEIGTPSVAEASCLLAAG
EESKLLKEKRIFKNQSGAVTIAVAESKNQYNPTHGEIHIIGSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRN
DQVLIESKLTEEKDRCSKAIKLAEEGIKVALISSGESGFYGMAGLLLELLQKIKIEYRPYFEVHPGISSVQLAAAISGAP
LMNDFCSISLSDKLTPWSLIEKRIAGALMGDFVIALFNPQSIERNWQLKIVIDICLKSRLGNTPVLIARQVGRENQSKKF
FTLNTIPFKEIDMLSIIIIGNSQTTLVDEIFLTPRGYLKN
>Mature_600_residues
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDAGKVNELIQVQKPKKILLKKWSEIDLIIFIGSIAASIRIINSFL
TSKDQDPSVIVIDKKCSKIVPLIGLHHSNTQNIAFQIANLFGGEIIETNNSNDQSFLNLDAFGYQWGWKRSGKIKDWSKL
VIKQAKNEEIFCKQLSGNSLWKTSESAEIINQINEKETEKPDSTFHVSIFKKHLTTWHPPVLWVGIGCERNTSKELIANS
FNNFLESGNFSQQSIAGFATIDIKKDEKGILELSKEKNLPIKFFSKEDLSTIIVPNPSTVVQKEIGTPSVAEASCLLAAG
EESKLLKEKRIFKNQSGAVTIAVAESKNQYNPTHGEIHIIGSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRN
DQVLIESKLTEEKDRCSKAIKLAEEGIKVALISSGESGFYGMAGLLLELLQKIKIEYRPYFEVHPGISSVQLAAAISGAP
LMNDFCSISLSDKLTPWSLIEKRIAGALMGDFVIALFNPQSIERNWQLKIVIDICLKSRLGNTPVLIARQVGRENQSKKF
FTLNTIPFKEIDMLSIIIIGNSQTTLVDEIFLTPRGYLKN

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 66836; Mature: 66836

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDAGKVNELIQVQKPKKILLKKWSEI
CCCEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCCCC
DLIIFIGSIAASIRIINSFLTSKDQDPSVIVIDKKCSKIVPLIGLHHSNTQNIAFQIANL
EEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCCHHHEEEHHH
FGGEIIETNNSNDQSFLNLDAFGYQWGWKRSGKIKDWSKLVIKQAKNEEIFCKQLSGNSL
CCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCC
WKTSESAEIINQINEKETEKPDSTFHVSIFKKHLTTWHPPVLWVGIGCERNTSKELIANS
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCEEEEEECCCCCCHHHHHHHH
FNNFLESGNFSQQSIAGFATIDIKKDEKGILELSKEKNLPIKFFSKEDLSTIIVPNPSTV
HHHHHHCCCCCHHHCCEEEEEEECCCCCCHHEECCCCCCCEEEECCCCCCEEEECCCCHH
VQKEIGTPSVAEASCLLAAGEESKLLKEKRIFKNQSGAVTIAVAESKNQYNPTHGEIHII
HHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEE
GSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRNDQVLIESKLTEEKDRCSKAI
ECCCCCEEEEECHHHHHHHHCEEEEHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHH
KLAEEGIKVALISSGESGFYGMAGLLLELLQKIKIEYRPYFEVHPGISSVQLAAAISGAP
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEHHHCCCC
LMNDFCSISLSDKLTPWSLIEKRIAGALMGDFVIALFNPQSIERNWQLKIVIDICLKSRL
HHHHHHCEEECCCCCHHHHHHHHHHHHHHHCEEEEEECCHHCCCCCEEEEEEEEHHHHHC
GNTPVLIARQVGRENQSKKFFTLNTIPFKEIDMLSIIIIGNSQTTLVDEIFLTPRGYLKN
CCCCEEEEHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEHEEECCCCCCCC
>Mature Secondary Structure
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDAGKVNELIQVQKPKKILLKKWSEI
CCCEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCCCC
DLIIFIGSIAASIRIINSFLTSKDQDPSVIVIDKKCSKIVPLIGLHHSNTQNIAFQIANL
EEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCCHHHEEEHHH
FGGEIIETNNSNDQSFLNLDAFGYQWGWKRSGKIKDWSKLVIKQAKNEEIFCKQLSGNSL
CCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCC
WKTSESAEIINQINEKETEKPDSTFHVSIFKKHLTTWHPPVLWVGIGCERNTSKELIANS
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCEEEEEECCCCCCHHHHHHHH
FNNFLESGNFSQQSIAGFATIDIKKDEKGILELSKEKNLPIKFFSKEDLSTIIVPNPSTV
HHHHHHCCCCCHHHCCEEEEEEECCCCCCHHEECCCCCCCEEEECCCCCCEEEECCCCHH
VQKEIGTPSVAEASCLLAAGEESKLLKEKRIFKNQSGAVTIAVAESKNQYNPTHGEIHII
HHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEE
GSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRNDQVLIESKLTEEKDRCSKAI
ECCCCCEEEEECHHHHHHHHCEEEEHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHH
KLAEEGIKVALISSGESGFYGMAGLLLELLQKIKIEYRPYFEVHPGISSVQLAAAISGAP
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEHHHCCCC
LMNDFCSISLSDKLTPWSLIEKRIAGALMGDFVIALFNPQSIERNWQLKIVIDICLKSRL
HHHHHHCEEECCCCCHHHHHHHHHHHHHHHCEEEEEECCHHCCCCCEEEEEEEEHHHHHC
GNTPVLIARQVGRENQSKKFFTLNTIPFKEIDMLSIIIIGNSQTTLVDEIFLTPRGYLKN
CCCCEEEEHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]