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The map label for this gene is pinE [H]

Identifier: 123969251

GI number: 123969251

Start: 1464500

End: 1465174

Strand: Direct

Name: pinE [H]

Synonym: A9601_17191

Alternate gene names: 123969251

Gene position: 1464500-1465174 (Clockwise)

Preceding gene: 123969249

Following gene: 123969252

Centisome position: 87.7

GC content: 26.81

Gene sequence:

>675_bases
GTGTTTTTTTATTTATTTATTGATACAGTAAAAAATATATGTAATAAAACATTGACTTTTAAATTTAAAAGAAAGCGTAT
TTTACTATCTGAAAAAAATAAAAACTCTAAAGCAATAGGTTATGCTAGAGCTACTAATAATGAATATGAATATTTAGAAG
AACAAATAAAATTTTTAAAGGGGGAGGGTTGCAGTTTAGTTTTCTCTGAGTTGATAAGTTTAGATGAAGAAATCAAACCC
CAACTCAATAAAGCTATAAATTGCTTGTCTAAAGGTGATCAATTAATAATAACTCAGCTTGATCGAGCGTTTAAAAATAA
AAAAGAATGTTTGAAGACAATAAATAAACTTATTAATAAGGATATTAAATTGCGAACTTTGACTGGTTTTTTTGCGGCTA
ATGAATTAACTAAAGCAAATTCTTCAATTTTTCAGATTTTATATGAATTAGATAATTTAGAAGAGAAAAGTTTAGGTGAA
AGAAAAAAAGAACAACTATTACGCAGAAAATTATCTGGGAATAATCTGGGAGGAAGGCCCAAAATAAGTCCTTTAAAAGA
ATCTTTAGTAATCAGATTACGTAATGAGGGATATTCATATCGATCAATCAGATCTCAAACTGGGATTGCATTATCAACAA
TAAGAAGAGTTATTTTGGAAGGAGAATTAACATAA

Upstream 100 bases:

>100_bases
CAGAATATAGAACGTGTTTTTTAATACAGATATAAGAACATGTTCTTATGGTTTTAATGAATAGAGTGTCTAATCTATTT
AAATTTTTTTGTACTAAAAA

Downstream 100 bases:

>100_bases
AATGAGGAATAAAGAAATTGAAAATTTAATTAAAGAAAATTTTAAAGATACAGCATTACGTATTTATGAATTAGATGATG
AAGATAGAAAAAGTTTGTTA

Product: site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MFFYLFIDTVKNICNKTLTFKFKRKRILLSEKNKNSKAIGYARATNNEYEYLEEQIKFLKGEGCSLVFSELISLDEEIKP
QLNKAINCLSKGDQLIITQLDRAFKNKKECLKTINKLINKDIKLRTLTGFFAANELTKANSSIFQILYELDNLEEKSLGE
RKKEQLLRRKLSGNNLGGRPKISPLKESLVIRLRNEGYSYRSIRSQTGIALSTIRRVILEGELT

Sequences:

>Translated_224_residues
MFFYLFIDTVKNICNKTLTFKFKRKRILLSEKNKNSKAIGYARATNNEYEYLEEQIKFLKGEGCSLVFSELISLDEEIKP
QLNKAINCLSKGDQLIITQLDRAFKNKKECLKTINKLINKDIKLRTLTGFFAANELTKANSSIFQILYELDNLEEKSLGE
RKKEQLLRRKLSGNNLGGRPKISPLKESLVIRLRNEGYSYRSIRSQTGIALSTIRRVILEGELT
>Mature_224_residues
MFFYLFIDTVKNICNKTLTFKFKRKRILLSEKNKNSKAIGYARATNNEYEYLEEQIKFLKGEGCSLVFSELISLDEEIKP
QLNKAINCLSKGDQLIITQLDRAFKNKKECLKTINKLINKDIKLRTLTGFFAANELTKANSSIFQILYELDNLEEKSLGE
RKKEQLLRRKLSGNNLGGRPKISPLKESLVIRLRNEGYSYRSIRSQTGIALSTIRRVILEGELT

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=176, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 25853; Mature: 25853

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFYLFIDTVKNICNKTLTFKFKRKRILLSEKNKNSKAIGYARATNNEYEYLEEQIKFLK
CEEEEEHHHHHHHHCHHHEEEEHHHHHEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHC
GEGCSLVFSELISLDEEIKPQLNKAINCLSKGDQLIITQLDRAFKNKKECLKTINKLINK
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DIKLRTLTGFFAANELTKANSSIFQILYELDNLEEKSLGERKKEQLLRRKLSGNNLGGRP
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
KISPLKESLVIRLRNEGYSYRSIRSQTGIALSTIRRVILEGELT
CCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFFYLFIDTVKNICNKTLTFKFKRKRILLSEKNKNSKAIGYARATNNEYEYLEEQIKFLK
CEEEEEHHHHHHHHCHHHEEEEHHHHHEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHC
GEGCSLVFSELISLDEEIKPQLNKAINCLSKGDQLIITQLDRAFKNKKECLKTINKLINK
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DIKLRTLTGFFAANELTKANSSIFQILYELDNLEEKSLGERKKEQLLRRKLSGNNLGGRP
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
KISPLKESLVIRLRNEGYSYRSIRSQTGIALSTIRRVILEGELT
CCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]