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The map label for this gene is rne [H]

Identifier: 123969236

GI number: 123969236

Start: 1448089

End: 1449909

Strand: Direct

Name: rne [H]

Synonym: A9601_17041

Alternate gene names: 123969236

Gene position: 1448089-1449909 (Clockwise)

Preceding gene: 123969233

Following gene: 123969237

Centisome position: 86.72

GC content: 31.19

Gene sequence:

>1821_bases
ATGTCTCAGCAAATTATCATCGCTGAGCAGGCTCGAATTGCAGCACTACTCACAGATGATCGAGTTGATGAATTAATCGT
CGCACAAGGTCAATATCAAATTGGCGATATTTTTTTAGGAACAGTTGAAAACGTTCTCCCTGGTATTGATGCAGCTTTCA
TAAATATCGGTGAAAGTGAGAAAAATGGATTCATCCATGTTTCAGATTTAGGTCCACTAAGACTCAAAAAAGGGACATTT
GGAATAACGGAATTACTAGAACCAAAACAAAAAGTCCTAGTGCAGGTAATAAAGGAACCTACAGGATCTAAAGGGCCTAG
ACTAACCGGGAGCATTTCAATCCCAGGGAAATACTTGATACTACAGCCATATGGACAAGGAGTAAATATTTCAAGAAAAA
TAAATACAGAAACAGAGCGAAGCCGTTTGAAAGCTCTTGGGGTTTTAATTAAACCACCTAGCACAGGCTTGTTATTTAGA
ACAGAGGCTGAAAAGATAAAAGAAGAACTTCTAATTGAAGATTTAGAACATTTAATTCAACAATGGGAAAATATACTAAA
AGTTTCTGAGGCTTCTAATCCGCCAAATTTAATAAAAAGAGATGATGATTTCTCTCTTAAGATCTTAAGAGATTATATTA
AAGAATCTACTAAAAGTTTAATTATCGATAGTAAATTTTCAGTTATAAGGGCAAAAGATTTTTTAATAAATTATGAGTCT
AATGTAGATATTGAATTTCACGATAACAGTTTAAACCAACATATTTTGGAGAAGTATGAAATTAAGAAAACAATTCAAAA
AGCTCTCCAGCCGAGAGTAGATCTTCCTTCGGGGGGTTATATAATTATTGAACCTACTGAAGCTTTAACAGTAATCGACG
TAAACTCTGGATCATTTACCAGATCCGCTAACTCAAGACAAACCGTTTTGTGGACGAACTGCGAAGCAGCAGTTGAAATC
TCAAGACAAATGAAATTAAGAAATATCGGTGGAGTTATAGTAGTTGATTTTATTGATATGGAATCTAGAAGAGATCAATT
TCAGTTACTTGAACATTTTACCTCAGCAATAAAAGATGATTCTGCTAGGCCTCAAATAGCTCAGCTTACTGAATTAGGTT
TAGTGGAGTTAACAAGAAAAAGACAAGGGCAAAATATATATGAACTATTCGGCAAAAAATGTTCTTCATGCGACGGTACA
GGACATGTAGAAAATATATTAAATCACGAAATTTCTTACTTAAAAATTAAAAATGTTGAAAATAAATCTAATCAATCAAA
CAATTTAACATCTTTAGATATAGTTACTTCTCAATCAACTGATGAGCAAGAAAAAATAATTGACAAGGAATTACTGAATT
CCAAAGACCTAAGTAAAGAAAATTCTTCGAATAAAAAAGAAAATGAAAATGATAATTTAAACCAATCAAATTCAAAAGAA
AAAAATATAATAACAGTTGATCTTACTAATGAAGAAAAAATTGTTTTCAGTGAATTGGGTATAAATCCACTTATAAAGTT
AGGTAAAGAATATCTAACTAGCAATAATTTTGTGCGATTAAAAGAGAGTAATAAGGAAACGGAAAAACCCTTAGATAATA
AAAAAGCAACATCAAAAAAAACTAAAAAAATCTCACAATCGGGAGAAGAAAAGATTCAAATTAAAATTGAAGCAAATGCA
AGTTCTAAAGATAAAACTTCAAATAAAACTAATGAAAGTAATGAAGTTGTATCTACAGATAAAAAGGATCAAATTGAACT
TACAGATGAGCTAAACAATGCAAGAAAAAAAAGAAGAAGATCTTCAGCAAGCATTGAATAA

Upstream 100 bases:

>100_bases
GTAAATCATAGAGGCTATAAGAATCGCTTTATTAAAAGATAAAAATCTAATTCTTTAATTTAGATGATTTTCAAAATCCA
AAACATACAGAATCAAATAT

Downstream 100 bases:

>100_bases
AGTATCAGAAGTTGGAATAGATGAAGTTGGAAAGGGAGCAATTTTTGGTCCAGTTTTTGCAGCAGCTGTAGTATTAACAG
AAAAAAATAAATTTATCTTA

Product: ribonuclease E/G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESEKNGFIHVSDLGPLRLKKGTF
GITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYLILQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLLFR
TEAEKIKEELLIEDLEHLIQQWENILKVSEASNPPNLIKRDDDFSLKILRDYIKESTKSLIIDSKFSVIRAKDFLINYES
NVDIEFHDNSLNQHILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSSCDGT
GHVENILNHEISYLKIKNVENKSNQSNNLTSLDIVTSQSTDEQEKIIDKELLNSKDLSKENSSNKKENENDNLNQSNSKE
KNIITVDLTNEEKIVFSELGINPLIKLGKEYLTSNNFVRLKESNKETEKPLDNKKATSKKTKKISQSGEEKIQIKIEANA
SSKDKTSNKTNESNEVVSTDKKDQIELTDELNNARKKRRRSSASIE

Sequences:

>Translated_606_residues
MSQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESEKNGFIHVSDLGPLRLKKGTF
GITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYLILQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLLFR
TEAEKIKEELLIEDLEHLIQQWENILKVSEASNPPNLIKRDDDFSLKILRDYIKESTKSLIIDSKFSVIRAKDFLINYES
NVDIEFHDNSLNQHILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSSCDGT
GHVENILNHEISYLKIKNVENKSNQSNNLTSLDIVTSQSTDEQEKIIDKELLNSKDLSKENSSNKKENENDNLNQSNSKE
KNIITVDLTNEEKIVFSELGINPLIKLGKEYLTSNNFVRLKESNKETEKPLDNKKATSKKTKKISQSGEEKIQIKIEANA
SSKDKTSNKTNESNEVVSTDKKDQIELTDELNNARKKRRRSSASIE
>Mature_605_residues
SQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESEKNGFIHVSDLGPLRLKKGTFG
ITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYLILQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLLFRT
EAEKIKEELLIEDLEHLIQQWENILKVSEASNPPNLIKRDDDFSLKILRDYIKESTKSLIIDSKFSVIRAKDFLINYESN
VDIEFHDNSLNQHILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEIS
RQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSSCDGTG
HVENILNHEISYLKIKNVENKSNQSNNLTSLDIVTSQSTDEQEKIIDKELLNSKDLSKENSSNKKENENDNLNQSNSKEK
NIITVDLTNEEKIVFSELGINPLIKLGKEYLTSNNFVRLKESNKETEKPLDNKKATSKKTKKISQSGEEKIQIKIEANAS
SKDKTSNKTNESNEVVSTDKKDQIELTDELNNARKKRRRSSASIE

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=394, Percent_Identity=34.2639593908629, Blast_Score=215, Evalue=8e-57,
Organism=Escherichia coli, GI87082235, Length=381, Percent_Identity=33.3333333333333, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 68246; Mature: 68115

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESE
CCCCEEEECCCEEEEEECCCCHHHHEEECCCEEECCEEHHHHHHHHCCCCEEEEECCCCC
KNGFIHVSDLGPLRLKKGTFGITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYLI
CCCEEEEECCCCEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLLFRTEAEKIKEELLIEDLEHLIQ
EEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWENILKVSEASNPPNLIKRDDDFSLKILRDYIKESTKSLIIDSKFSVIRAKDFLINYES
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECC
NVDIEFHDNSLNQHILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
SARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSSCDGTGHVENILNHEISYLKIKNVE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEEECCC
NKSNQSNNLTSLDIVTSQSTDEQEKIIDKELLNSKDLSKENSSNKKENENDNLNQSNSKE
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KNIITVDLTNEEKIVFSELGINPLIKLGKEYLTSNNFVRLKESNKETEKPLDNKKATSKK
CEEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHHHH
TKKISQSGEEKIQIKIEANASSKDKTSNKTNESNEVVSTDKKDQIELTDELNNARKKRRR
HHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHHHH
SSASIE
HCCCCC
>Mature Secondary Structure 
SQQIIIAEQARIAALLTDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESE
CCCEEEECCCEEEEEECCCCHHHHEEECCCEEECCEEHHHHHHHHCCCCEEEEECCCCC
KNGFIHVSDLGPLRLKKGTFGITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYLI
CCCEEEEECCCCEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLLFRTEAEKIKEELLIEDLEHLIQ
EEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWENILKVSEASNPPNLIKRDDDFSLKILRDYIKESTKSLIIDSKFSVIRAKDFLINYES
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECC
NVDIEFHDNSLNQHILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
SARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSSCDGTGHVENILNHEISYLKIKNVE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEEECCC
NKSNQSNNLTSLDIVTSQSTDEQEKIIDKELLNSKDLSKENSSNKKENENDNLNQSNSKE
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KNIITVDLTNEEKIVFSELGINPLIKLGKEYLTSNNFVRLKESNKETEKPLDNKKATSKK
CEEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHHHH
TKKISQSGEEKIQIKIEANASSKDKTSNKTNESNEVVSTDKKDQIELTDELNNARKKRRR
HHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHHHH
SSASIE
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]