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The map label for this gene is rlmN

Identifier: 123969217

GI number: 123969217

Start: 1422837

End: 1423883

Strand: Reverse

Name: rlmN

Synonym: A9601_16851

Alternate gene names: 123969217

Gene position: 1423883-1422837 (Counterclockwise)

Preceding gene: 123969218

Following gene: 123969215

Centisome position: 85.27

GC content: 31.23

Gene sequence:

>1047_bases
TTGAAAAATCTTCTTGGAAGTAGTATTAATGATTTAGAAAATGTAGCTTTAGATTATGGGCAGGCTGCTTTCAGGGGTCG
CCAAATCTATAACTGGATTTATAACTATAGAAATAAGAACAAAAATATTGATCAAATAGAAGTTTTACCTTTAGATTTTA
GAGAGAAGTTAAAGGTTGATGGTTTTAAGGTTAGTGAGCTGGTTATTAAAGAAAGAAACTTAGCTAACGATGGTACTTTA
AAGTTGTTACTTTCTACAGAAGATAATGAAAGTATTGAGTGCGTTGGTATACCAACTGAAAAAAGATTAACTGCTTGTCT
TTCTAGTCAAGTTGGTTGTCCAATGGACTGCAAATTTTGTGCAACTGGTAAGGAAGGTTTGAAGAGATCTTTAAAAGCAA
GTGAAATTTTAGATCAAATTTTATTTATCGAATATGAAATGAACAGAAAAGTGACTAATATTGTTTTCATGGGTATGGGT
GAGCCCTTATTGAATATTGATGAATTACTTTTGTCAATTAGATCTATAAATAATGATTTTCAGATCAGTCAGAGAAAGAT
AACTGTAAGCACAGTAGCTATTCCAAAAATGATAAGTAAATTATCAGCAAAATCTTTTCAGATTTTGAGTAATTGTCAAT
TTACATTAGCAATTAGCCTACATGCTTCAAATCAAAAAACAAGAGAGACCATAATACCAAGTGCAAAAAACTATGAAATC
AAAAATATAATCGAAGACTGTAAGACATTCGTAAGAGAAACTGGTCGAAGGGTAAGTTTTGAATATCTAATGCTAAGTGG
GGTAAATGATAAATTAGAACATGCTTGTGAATTGAGTAATTTGCTGAAAGGTTTTCAATGTCATGTAAATTTAATCCAGT
ATAACCAAATTGACGAGGTTGAATTTCAACGAACCTCTTTAAAAAATCTTCAATTATTCCAATCTAGACTTGTTAACAAT
GGCATCGCTGTTAGCTTGCGAAAAAGCAGAGGTCTAGATAAAAATGCTGCATGTGGTCAGCTAAGACAAAATGCAAAAAG
TAAATAG

Upstream 100 bases:

>100_bases
ATGGCTATGATTGGTTTTATTGCCCTTATTCTTACAGAATTCTTTTTAAAACATGGTTTGCTGTTATGGTGAAAACTGTT
TTAAATAAAGACTATTAAAA

Downstream 100 bases:

>100_bases
TATTATCTAATAAAAATTTTTTAAAAGTCTCTTAAACAGGTTATTATTGTGTTAATTAATCTTAAATCTTTGAGTTTTAT
TAAAACAAAAATTTTACCTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKNLLGSSINDLENVALDYGQAAFRGRQIYNWIYNYRNKNKNIDQIEVLPLDFREKLKVDGFKVSELVIKERNLANDGTL
KLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIEYEMNRKVTNIVFMGMG
EPLLNIDELLLSIRSINNDFQISQRKITVSTVAIPKMISKLSAKSFQILSNCQFTLAISLHASNQKTRETIIPSAKNYEI
KNIIEDCKTFVRETGRRVSFEYLMLSGVNDKLEHACELSNLLKGFQCHVNLIQYNQIDEVEFQRTSLKNLQLFQSRLVNN
GIAVSLRKSRGLDKNAACGQLRQNAKSK

Sequences:

>Translated_348_residues
MKNLLGSSINDLENVALDYGQAAFRGRQIYNWIYNYRNKNKNIDQIEVLPLDFREKLKVDGFKVSELVIKERNLANDGTL
KLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIEYEMNRKVTNIVFMGMG
EPLLNIDELLLSIRSINNDFQISQRKITVSTVAIPKMISKLSAKSFQILSNCQFTLAISLHASNQKTRETIIPSAKNYEI
KNIIEDCKTFVRETGRRVSFEYLMLSGVNDKLEHACELSNLLKGFQCHVNLIQYNQIDEVEFQRTSLKNLQLFQSRLVNN
GIAVSLRKSRGLDKNAACGQLRQNAKSK
>Mature_348_residues
MKNLLGSSINDLENVALDYGQAAFRGRQIYNWIYNYRNKNKNIDQIEVLPLDFREKLKVDGFKVSELVIKERNLANDGTL
KLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIEYEMNRKVTNIVFMGMG
EPLLNIDELLLSIRSINNDFQISQRKITVSTVAIPKMISKLSAKSFQILSNCQFTLAISLHASNQKTRETIIPSAKNYEI
KNIIEDCKTFVRETGRRVSFEYLMLSGVNDKLEHACELSNLLKGFQCHVNLIQYNQIDEVEFQRTSLKNLQLFQSRLVNN
GIAVSLRKSRGLDKNAACGQLRQNAKSK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=337, Percent_Identity=35.9050445103858, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PROMS (A2BT57)

Other databases:

- EMBL:   CP000551
- RefSeq:   YP_001010075.1
- STRING:   A2BT57
- GeneID:   4718415
- GenomeReviews:   CP000551_GR
- KEGG:   pmb:A9601_16851
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   CKERITI
- ProtClustDB:   PRK14457
- BioCyc:   PMAR146891:A9601_16851-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39490; Mature: 39490

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLGSSINDLENVALDYGQAAFRGRQIYNWIYNYRNKNKNIDQIEVLPLDFREKLKVD
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCC
GFKVSELVIKERNLANDGTLKLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFC
CEEHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKASEILDQILFIEYEMNRKVTNIVFMGMGEPLLNIDELLLSIRSINNDF
CCCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCC
QISQRKITVSTVAIPKMISKLSAKSFQILSNCQFTLAISLHASNQKTRETIIPSAKNYEI
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHCCCCCCCHH
KNIIEDCKTFVRETGRRVSFEYLMLSGVNDKLEHACELSNLLKGFQCHVNLIQYNQIDEV
HHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
EFQRTSLKNLQLFQSRLVNNGIAVSLRKSRGLDKNAACGQLRQNAKSK
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKNLLGSSINDLENVALDYGQAAFRGRQIYNWIYNYRNKNKNIDQIEVLPLDFREKLKVD
CCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCC
GFKVSELVIKERNLANDGTLKLLLSTEDNESIECVGIPTEKRLTACLSSQVGCPMDCKFC
CEEHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKASEILDQILFIEYEMNRKVTNIVFMGMGEPLLNIDELLLSIRSINNDF
CCCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCC
QISQRKITVSTVAIPKMISKLSAKSFQILSNCQFTLAISLHASNQKTRETIIPSAKNYEI
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHCCCCCCCHH
KNIIEDCKTFVRETGRRVSFEYLMLSGVNDKLEHACELSNLLKGFQCHVNLIQYNQIDEV
HHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
EFQRTSLKNLQLFQSRLVNNGIAVSLRKSRGLDKNAACGQLRQNAKSK
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA