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The map label for this gene is purD [H]

Identifier: 123969073

GI number: 123969073

Start: 1320104

End: 1321435

Strand: Direct

Name: purD [H]

Synonym: A9601_15411

Alternate gene names: 123969073

Gene position: 1320104-1321435 (Clockwise)

Preceding gene: 123969064

Following gene: 123969074

Centisome position: 79.05

GC content: 31.91

Gene sequence:

>1332_bases
ATGGGAAGTCATTCACCAAGTTCTAGGAGCTCTATTAGATTAGAAAATATATTAATAATTGGAAATGGCGGGAGAGAAAA
CTCTTTGGCTTGGGCTATTCAAAAAAATGAATTAGTTAAAAAAGTTTATTTAATACCTGGCAACGCTGGTTCAGAAAGAA
TAAATAAATGTAAAAGAATAAAAATTGATATAAATAATAAAAATGAACTAGTTGAAAAGCTTGATTTTTTGAAAATAGAT
TTAGTTGTTATCGGACCAGAAATACCTCTAGCAAATGGATTAGCTGATTTTCTTCGCAAAAAAAATTTTAAAGTATTTGG
TCCTAATAAAGATGGAGCAAAATTAGAATTTAGTAAATCTTGGGCAAAGGAATTTATGCAAGACGCAAATATTCCAACTG
CAAACTTTTGGAAAGTCAATTCTTTAGAAGAAGCCAAAAAAATCATCAATTCAGCATCAATTCCATTGGTAGTAAAAGCA
GATGGTCTAGCTTCAGGTAAGGGTGTTTTTATTCCTAATTCAAAAGATGAATGCTTTGCAGCTGCAGAGTCAATTTTTAA
TGGTAGATTTGGCAACTCTGGGAATATAGTTGTTCTAGAAGAAAAAATTCAAGGGCCAGAAGTTTCAGTTTTTGCTTTAT
GCGATGGGAAGAGATACACATTACTTCCAACCGCCCAAGATCACAAAAGACTTAATGAGAAAGATAAAGGACCAAATACA
GGTGGTATGGGAGCCTATTCTCCTGCCCCATTATTAACAGAAGCTTACCTTGACAGAATTATTAAAGAGATAATTGAACC
TACAATAGATGAATTAAATAAAAGAAATATTGACTATAGAGGTGTAATTTACTTTGGGTTAATGATCACAGAATCTGGGC
CTAAAGTTATAGAATACAATTGCAGATTTGGTGATCCAGAATGCCAGACAATAATGCCTTTGATGGATCAGAATTTCGTA
TTTCTTTTAGAGAAATGTTCAATGGGAAATTTAACTAGTGGTGAAAAAATTAATACATCTGATAAAGTTAGCGGTTGTGT
AATAGCGACCTCACAAGGTTATCCTTTCGAATATGAAACTGGATTTGAAATAAATATAGGTAAGATTGATTCGAATGATT
GTCAAATTTTCGACTCAGGTACCTCTTCTAATGAAAATGGGAAATTATTAACTAGTGGAGGAAGAGTCTTAAGTGTTGTC
TGCCAAGATAAAGATTTCGATTTGGTTTTTGAAAAAGCATACAAGAATTTAAAAAAAATCCATTTTAAGGGAATTTACTT
CAGAAATGACATAGGTTATCAAGTCAGAAAAAACTTTTCAAAGGAGAATTAA

Upstream 100 bases:

>100_bases
AACATAAATATTAGAGATCAATCTTAAATGATCTTAAATGATTTCATTTCATCTTAACTGATTATTCATCAAAACCTCAA
AACCTTAAAAAAACGAATAT

Downstream 100 bases:

>100_bases
GATTATGGTGGGTTCCAATAATCAAGAAAGTGCGAAATATAGCATCAATGATAATGAAGAAATGAATAAAAACCATTGGA
TTAATGGACTCCTCGCTTGG

Product: phosphoribosylglycinamide synthetase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MGSHSPSSRSSIRLENILIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCKRIKIDINNKNELVEKLDFLKID
LVVIGPEIPLANGLADFLRKKNFKVFGPNKDGAKLEFSKSWAKEFMQDANIPTANFWKVNSLEEAKKIINSASIPLVVKA
DGLASGKGVFIPNSKDECFAAAESIFNGRFGNSGNIVVLEEKIQGPEVSVFALCDGKRYTLLPTAQDHKRLNEKDKGPNT
GGMGAYSPAPLLTEAYLDRIIKEIIEPTIDELNKRNIDYRGVIYFGLMITESGPKVIEYNCRFGDPECQTIMPLMDQNFV
FLLEKCSMGNLTSGEKINTSDKVSGCVIATSQGYPFEYETGFEINIGKIDSNDCQIFDSGTSSNENGKLLTSGGRVLSVV
CQDKDFDLVFEKAYKNLKKIHFKGIYFRNDIGYQVRKNFSKEN

Sequences:

>Translated_443_residues
MGSHSPSSRSSIRLENILIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCKRIKIDINNKNELVEKLDFLKID
LVVIGPEIPLANGLADFLRKKNFKVFGPNKDGAKLEFSKSWAKEFMQDANIPTANFWKVNSLEEAKKIINSASIPLVVKA
DGLASGKGVFIPNSKDECFAAAESIFNGRFGNSGNIVVLEEKIQGPEVSVFALCDGKRYTLLPTAQDHKRLNEKDKGPNT
GGMGAYSPAPLLTEAYLDRIIKEIIEPTIDELNKRNIDYRGVIYFGLMITESGPKVIEYNCRFGDPECQTIMPLMDQNFV
FLLEKCSMGNLTSGEKINTSDKVSGCVIATSQGYPFEYETGFEINIGKIDSNDCQIFDSGTSSNENGKLLTSGGRVLSVV
CQDKDFDLVFEKAYKNLKKIHFKGIYFRNDIGYQVRKNFSKEN
>Mature_442_residues
GSHSPSSRSSIRLENILIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCKRIKIDINNKNELVEKLDFLKIDL
VVIGPEIPLANGLADFLRKKNFKVFGPNKDGAKLEFSKSWAKEFMQDANIPTANFWKVNSLEEAKKIINSASIPLVVKAD
GLASGKGVFIPNSKDECFAAAESIFNGRFGNSGNIVVLEEKIQGPEVSVFALCDGKRYTLLPTAQDHKRLNEKDKGPNTG
GMGAYSPAPLLTEAYLDRIIKEIIEPTIDELNKRNIDYRGVIYFGLMITESGPKVIEYNCRFGDPECQTIMPLMDQNFVF
LLEKCSMGNLTSGEKINTSDKVSGCVIATSQGYPFEYETGFEINIGKIDSNDCQIFDSGTSSNENGKLLTSGGRVLSVVC
QDKDFDLVFEKAYKNLKKIHFKGIYFRNDIGYQVRKNFSKEN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=422, Percent_Identity=41.4691943127962, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI4503915, Length=422, Percent_Identity=41.4691943127962, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI209869995, Length=422, Percent_Identity=41.4691943127962, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI209869993, Length=422, Percent_Identity=41.4691943127962, Blast_Score=336, Evalue=3e-92,
Organism=Escherichia coli, GI1790438, Length=422, Percent_Identity=38.6255924170616, Blast_Score=305, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI17567511, Length=431, Percent_Identity=43.3874709976798, Blast_Score=295, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=37.4439461883408, Blast_Score=286, Evalue=4e-78,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=39.8148148148148, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=40.5092592592593, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 49238; Mature: 49107

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSHSPSSRSSIRLENILIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCKRI
CCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHCCCEEE
KIDINNKNELVEKLDFLKIDLVVIGPEIPLANGLADFLRKKNFKVFGPNKDGAKLEFSKS
EEEECCHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEECHH
WAKEFMQDANIPTANFWKVNSLEEAKKIINSASIPLVVKADGLASGKGVFIPNSKDECFA
HHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCHHHHH
AAESIFNGRFGNSGNIVVLEEKIQGPEVSVFALCDGKRYTLLPTAQDHKRLNEKDKGPNT
HHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCC
GGMGAYSPAPLLTEAYLDRIIKEIIEPTIDELNKRNIDYRGVIYFGLMITESGPKVIEYN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEE
CRFGDPECQTIMPLMDQNFVFLLEKCSMGNLTSGEKINTSDKVSGCVIATSQGYPFEYET
CCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECC
GFEINIGKIDSNDCQIFDSGTSSNENGKLLTSGGRVLSVVCQDKDFDLVFEKAYKNLKKI
CEEEEEEEECCCCCEEEECCCCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHE
HFKGIYFRNDIGYQVRKNFSKEN
EEEEEEEECCCCHHHHHCCCCCC
>Mature Secondary Structure 
GSHSPSSRSSIRLENILIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCKRI
CCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHCCCEEE
KIDINNKNELVEKLDFLKIDLVVIGPEIPLANGLADFLRKKNFKVFGPNKDGAKLEFSKS
EEEECCHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEECHH
WAKEFMQDANIPTANFWKVNSLEEAKKIINSASIPLVVKADGLASGKGVFIPNSKDECFA
HHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCHHHHH
AAESIFNGRFGNSGNIVVLEEKIQGPEVSVFALCDGKRYTLLPTAQDHKRLNEKDKGPNT
HHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCC
GGMGAYSPAPLLTEAYLDRIIKEIIEPTIDELNKRNIDYRGVIYFGLMITESGPKVIEYN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEE
CRFGDPECQTIMPLMDQNFVFLLEKCSMGNLTSGEKINTSDKVSGCVIATSQGYPFEYET
CCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECC
GFEINIGKIDSNDCQIFDSGTSSNENGKLLTSGGRVLSVVCQDKDFDLVFEKAYKNLKKI
CEEEEEEEECCCCCEEEECCCCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHE
HFKGIYFRNDIGYQVRKNFSKEN
EEEEEEEECCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]