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The map label for this gene is thiE [H]

Identifier: 123969005

GI number: 123969005

Start: 1256992

End: 1258047

Strand: Direct

Name: thiE [H]

Synonym: A9601_14731

Alternate gene names: 123969005

Gene position: 1256992-1258047 (Clockwise)

Preceding gene: 123969004

Following gene: 123969006

Centisome position: 75.27

GC content: 29.73

Gene sequence:

>1056_bases
ATGCTGAATTCCAATACTAAAGACCATGAAGATTTAAGAATTTATCAGATTATTGACGCTAATCTAGATAGAGCAAGAGA
AGGACTAAGAGTATTAGAGGATTGGGCTAGATTTGGTCTAGGCAAAGAAAAATATGTTGAAAAAATTAAAAATTTTAGAC
AAATTTTAGGAAAAAATCATTTAGAAGTTTATAAACAATCTAGAAATCAGATCGAAGACAATTGTAAAGGATTGACTCAT
CAAGAGCAATTAAACAGAAAAACTTCTGAGCAAATTATAAGTTCTAATTCAGCAAGAGTTCAAGAAGCATTACGAGTCAT
AGAGGAATTCTCAAGGCTGCACAATAATGAGCTTTCAAAAATCGCTTCTGAAATTAGATATGAAATTTATACTGTTGAAA
TTGACCTATTAAGTTTTAGCAAGTTTAAGAAGTCGGAGAAAATATTAAAAGAAAATGACTTATATGTAATCACAGATCAA
AAGGACAATTTATTAGAAATAATTGAAGAGATTTTAATTGCGGGAGTAAAAATTATTCAGCATAGATTTAAAACGGGAAC
TGATCAAGATCATCTTCAAGAAGCAATTGAGATTAAAAATCTATGTAAAAGATATAATTCTTTGTTCATAGTTAACGATA
GACTTGATATAGCTCTAGCATCTAACGCGGATGGGATTCATCTTGGACAAGACGATTTAGACTTAAAAACCACAAGAAAG
CTATTTGGATATTCAAAAATAATCGGTATAAGTGCAAATAATGCAATTGATATTTCAAATGCTCTTGACGAGGGTTGTGA
CTACATAGGGATAGGGCCAGTATTTGAAACTACGACAAAAAAGAATAAAAAACCTTTAGGTATTGAAAATATCAAAACAT
TAACAAAGGATTTAAATATTCCTTGGTTTGCTATCGGAGGAATCAAGTCAAATAACATTTCATATTTAAAAAGAAATGGG
TTTAAAAAAGTTGCCTTAGTTTCGGAATTAATGAATTCTGAAGATCCTAAAGAAGACGCTATGATGATTTTAAAAGAATT
GTCTCATGAAAATTAG

Upstream 100 bases:

>100_bases
CAAATCAAGTTCAAAAATATAGATCAACTTTCTAATCAAATTAAAATAGATAGAGATAATGCTCTGAAAATTTTTAAAAA
CTACAAAAAATAAATTACAA

Downstream 100 bases:

>100_bases
GGTAAATGGAGAAGAAAAAAAAATAGAACTTGATCAAGAAAATGCTCTACTATCTAAAGCTCTTCACCATATGGGATATA
AACCAAACACAATTGTAGTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MLNSNTKDHEDLRIYQIIDANLDRAREGLRVLEDWARFGLGKEKYVEKIKNFRQILGKNHLEVYKQSRNQIEDNCKGLTH
QEQLNRKTSEQIISSNSARVQEALRVIEEFSRLHNNELSKIASEIRYEIYTVEIDLLSFSKFKKSEKILKENDLYVITDQ
KDNLLEIIEEILIAGVKIIQHRFKTGTDQDHLQEAIEIKNLCKRYNSLFIVNDRLDIALASNADGIHLGQDDLDLKTTRK
LFGYSKIIGISANNAIDISNALDEGCDYIGIGPVFETTTKKNKKPLGIENIKTLTKDLNIPWFAIGGIKSNNISYLKRNG
FKKVALVSELMNSEDPKEDAMMILKELSHEN

Sequences:

>Translated_351_residues
MLNSNTKDHEDLRIYQIIDANLDRAREGLRVLEDWARFGLGKEKYVEKIKNFRQILGKNHLEVYKQSRNQIEDNCKGLTH
QEQLNRKTSEQIISSNSARVQEALRVIEEFSRLHNNELSKIASEIRYEIYTVEIDLLSFSKFKKSEKILKENDLYVITDQ
KDNLLEIIEEILIAGVKIIQHRFKTGTDQDHLQEAIEIKNLCKRYNSLFIVNDRLDIALASNADGIHLGQDDLDLKTTRK
LFGYSKIIGISANNAIDISNALDEGCDYIGIGPVFETTTKKNKKPLGIENIKTLTKDLNIPWFAIGGIKSNNISYLKRNG
FKKVALVSELMNSEDPKEDAMMILKELSHEN
>Mature_351_residues
MLNSNTKDHEDLRIYQIIDANLDRAREGLRVLEDWARFGLGKEKYVEKIKNFRQILGKNHLEVYKQSRNQIEDNCKGLTH
QEQLNRKTSEQIISSNSARVQEALRVIEEFSRLHNNELSKIASEIRYEIYTVEIDLLSFSKFKKSEKILKENDLYVITDQ
KDNLLEIIEEILIAGVKIIQHRFKTGTDQDHLQEAIEIKNLCKRYNSLFIVNDRLDIALASNADGIHLGQDDLDLKTTRK
LFGYSKIIGISANNAIDISNALDEGCDYIGIGPVFETTTKKNKKPLGIENIKTLTKDLNIPWFAIGGIKSNNISYLKRNG
FKKVALVSELMNSEDPKEDAMMILKELSHEN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=186, Percent_Identity=30.1075268817204, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325042, Length=206, Percent_Identity=34.9514563106796, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 40311; Mature: 40311

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSNTKDHEDLRIYQIIDANLDRAREGLRVLEDWARFGLGKEKYVEKIKNFRQILGKNH
CCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
LEVYKQSRNQIEDNCKGLTHQEQLNRKTSEQIISSNSARVQEALRVIEEFSRLHNNELSK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHH
IASEIRYEIYTVEIDLLSFSKFKKSEKILKENDLYVITDQKDNLLEIIEEILIAGVKIIQ
HHHHHHEEEEEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
HRFKTGTDQDHLQEAIEIKNLCKRYNSLFIVNDRLDIALASNADGIHLGQDDLDLKTTRK
HHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHH
LFGYSKIIGISANNAIDISNALDEGCDYIGIGPVFETTTKKNKKPLGIENIKTLTKDLNI
HHHHHHEEEECCCCCEECHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PWFAIGGIKSNNISYLKRNGFKKVALVSELMNSEDPKEDAMMILKELSHEN
CEEEECCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNSNTKDHEDLRIYQIIDANLDRAREGLRVLEDWARFGLGKEKYVEKIKNFRQILGKNH
CCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
LEVYKQSRNQIEDNCKGLTHQEQLNRKTSEQIISSNSARVQEALRVIEEFSRLHNNELSK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHH
IASEIRYEIYTVEIDLLSFSKFKKSEKILKENDLYVITDQKDNLLEIIEEILIAGVKIIQ
HHHHHHEEEEEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
HRFKTGTDQDHLQEAIEIKNLCKRYNSLFIVNDRLDIALASNADGIHLGQDDLDLKTTRK
HHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHH
LFGYSKIIGISANNAIDISNALDEGCDYIGIGPVFETTTKKNKKPLGIENIKTLTKDLNI
HHHHHHEEEECCCCCEECHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PWFAIGGIKSNNISYLKRNGFKKVALVSELMNSEDPKEDAMMILKELSHEN
CEEEECCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]