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The map label for this gene is hisH [H]

Identifier: 123968698

GI number: 123968698

Start: 977407

End: 978024

Strand: Reverse

Name: hisH [H]

Synonym: A9601_11651

Alternate gene names: 123968698

Gene position: 978024-977407 (Counterclockwise)

Preceding gene: 123968699

Following gene: 123968697

Centisome position: 58.57

GC content: 32.36

Gene sequence:

>618_bases
TTGCATAAAATCGGACTAATAGACTATGGAATGGGTAATATTCATTCTGTAACGAAATCTCTAGAAAGTCTTGGAGAAGA
AATTATATTAATTAAAAACTTTAATGATTCTAAGCTTTGTAAGGCGATAATACTTCCTGGGGTTGGAGCATTTGATCCAG
CGATGAATAATCTCATAAATACTGATTTGATAAATGATTTGAAAAATTGGATTAAAAGTGGGAAGTCCTTTTTTGGGATA
TGTTTAGGTCTCCAACTCCTTTTTGAATCTAGTGATGAAGGAAAAGTTCAAGGGCTTGGAATTTTAAAAGGAAAAATACA
AAAAATACCCAATATTGTTAACCAAAGAATCCCACACATGGGTTGGTGCCAACTTATACCTACAAAAAAAAATACTCTTT
TTGGGATTGAAGAATTAAATAATTGGGTCTATTTTGTACATTCCTATCATGCAATCCCAGATGACTTAAATATTATTGCA
GCTCAGGTTGATTATGGCCCTGAAAAATTAACTGCAATGATCGAGAATGATAATTTATTAGCCTGTCAATTTCATCCGGA
AAAATCTGGAAAAACCGGAGAAAAACTTTTGAGACGATGGCTTAGCAATATTCAATAA

Upstream 100 bases:

>100_bases
TGATTTTTAAAGGAGGTCAAAAGGTTGATACCGTTGTTGGTGCTGTGCCAAAAGCAACTCTTTCGAGCACTTTAACTAAG
CATTTATAAATTTAAAAGCT

Downstream 100 bases:

>100_bases
TTGATAATTACTGATGAAGACAAACTTAAGATTAATAGGTGGTAAAAAACTCCAAAGTCCAAATAATTCCAATACCAGAC
CTACAACTTTGAGAGTAAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLINTDLINDLKNWIKSGKSFFGI
CLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHMGWCQLIPTKKNTLFGIEELNNWVYFVHSYHAIPDDLNIIA
AQVDYGPEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ

Sequences:

>Translated_205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLINTDLINDLKNWIKSGKSFFGI
CLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHMGWCQLIPTKKNTLFGIEELNNWVYFVHSYHAIPDDLNIIA
AQVDYGPEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ
>Mature_205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLINTDLINDLKNWIKSGKSFFGI
CLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHMGWCQLIPTKKNTLFGIEELNNWVYFVHSYHAIPDDLNIIA
AQVDYGPEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=37.3737373737374, Blast_Score=112, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=215, Percent_Identity=36.2790697674419, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22972; Mature: 22972

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLIN
CCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHH
TDLINDLKNWIKSGKSFFGICLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCC
GWCQLIPTKKNTLFGIEELNNWVYFVHSYHAIPDDLNIIAAQVDYGPEKLTAMIENDNLL
CCEEEECCCCCCEECHHHHCCEEEEEEECCCCCCCCEEEEEECCCCHHHEEEEECCCCEE
ACQFHPEKSGKTGEKLLRRWLSNIQ
EEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLIN
CCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHH
TDLINDLKNWIKSGKSFFGICLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCC
GWCQLIPTKKNTLFGIEELNNWVYFVHSYHAIPDDLNIIAAQVDYGPEKLTAMIENDNLL
CCEEEECCCCCCEECHHHHCCEEEEEEECCCCCCCCEEEEEECCCCHHHEEEEECCCCEE
ACQFHPEKSGKTGEKLLRRWLSNIQ
EEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA