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The map label for this gene is lasT [C]

Identifier: 123968694

GI number: 123968694

Start: 975507

End: 976259

Strand: Reverse

Name: lasT [C]

Synonym: A9601_11611

Alternate gene names: 123968694

Gene position: 976259-975507 (Counterclockwise)

Preceding gene: 123968695

Following gene: 123968693

Centisome position: 58.46

GC content: 27.62

Gene sequence:

>753_bases
ATGATTTTGGGAAAAAATTTTTCTAATTTAAAGGTAATATTAGTTGAGCCAAATGGCCCTTTAAATGTAGGTAGCGTCGC
TAGATTATGCAGTAATTTTGAAGTTGATGAATTAAGAATCGTCTCTCCTAAATGCGATATTTTTTCTTTAGAAGCAAAAA
AAATGGCCCTTAAAGGTCAAAAAATTCTTGAAAATTGTAAGATTTTTGATGATCTTCAAAAGGCAATTTTTGATTGTGAT
TTAGTATTAGCATCTTGTGGAAGGATTGATGTAAATAAAGATTCATTTTTTGTATCTTCTGAAGATATATTTGATTGGAC
TTTATCCTTTAATAAGTTAAATAATTTAGCAATTATATTTGGCAGGGAAGATAGAGGTTTAACTAACAGTGAATTGCTTC
TGGCAAATAAAACTTTTAGTATCCCAACTTCTCAGAATAATCCTTCATTAAATCTTTCTCACGCTGTTTCAATAGTTTTA
TATGAATTGAATAAGTCTTCTAAAAAGAATTTGAATAATGAATTAAAAGTTTTTAACTTAGCATCATCTAAGGAAGTACA
TGATACATTTCTTGATATAGAGGAAATGCTTTTGCGAGTTGGATATCTCTTAAAACATACCTCTAAGGCAAAAATCAGTA
AATTTAAGAATTTTATTTTGAGGGCAAATACATCAATGCACGAAATAAATGTTTTAAGAGGAATTGTCCATCAAATAAAT
TGGTTTTTGAACAATTCAAAAAAAAATAAGTAA

Upstream 100 bases:

>100_bases
TAATAAAACAAGTTACTGGAGGCCTAACTCCTCCTATGCCAAGCTTTGAAATTGATCCTGTAAATATGTCTAATTTATTA
AAATATCTTCATAGCCTTGA

Downstream 100 bases:

>100_bases
GTATAAATTTGATTAGTTATTGTTCTCTTCTTGTTTTAATTTGATAGGAATATTTACTAAAAACATTTTCTGAAGTAGCA
TCTATTGATTATTTGAATAT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEAKKMALKGQKILENCKIFDDLQKAIFDCD
LVLASCGRIDVNKDSFFVSSEDIFDWTLSFNKLNNLAIIFGREDRGLTNSELLLANKTFSIPTSQNNPSLNLSHAVSIVL
YELNKSSKKNLNNELKVFNLASSKEVHDTFLDIEEMLLRVGYLLKHTSKAKISKFKNFILRANTSMHEINVLRGIVHQIN
WFLNNSKKNK

Sequences:

>Translated_250_residues
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEAKKMALKGQKILENCKIFDDLQKAIFDCD
LVLASCGRIDVNKDSFFVSSEDIFDWTLSFNKLNNLAIIFGREDRGLTNSELLLANKTFSIPTSQNNPSLNLSHAVSIVL
YELNKSSKKNLNNELKVFNLASSKEVHDTFLDIEEMLLRVGYLLKHTSKAKISKFKNFILRANTSMHEINVLRGIVHQIN
WFLNNSKKNK
>Mature_250_residues
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEAKKMALKGQKILENCKIFDDLQKAIFDCD
LVLASCGRIDVNKDSFFVSSEDIFDWTLSFNKLNNLAIIFGREDRGLTNSELLLANKTFSIPTSQNNPSLNLSHAVSIVL
YELNKSSKKNLNNELKVFNLASSKEVHDTFLDIEEMLLRVGYLLKHTSKAKISKFKNFILRANTSMHEINVLRGIVHQIN
WFLNNSKKNK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=155, Percent_Identity=30.3225806451613, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28311; Mature: 28311

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEAKKMALKGQ
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEHHHHHHHHHH
KILENCKIFDDLQKAIFDCDLVLASCGRIDVNKDSFFVSSEDIFDWTLSFNKLNNLAIIF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCEEEEEEEECCCCCEEEEE
GREDRGLTNSELLLANKTFSIPTSQNNPSLNLSHAVSIVLYELNKSSKKNLNNELKVFNL
ECCCCCCCCCEEEEEECEEECCCCCCCCCEEHHHHEEEEEEECCCCHHHCCCCCEEEEEE
ASSKEVHDTFLDIEEMLLRVGYLLKHTSKAKISKFKNFILRANTSMHEINVLRGIVHQIN
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
WFLNNSKKNK
HHHCCCCCCC
>Mature Secondary Structure
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEAKKMALKGQ
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEHHHHHHHHHH
KILENCKIFDDLQKAIFDCDLVLASCGRIDVNKDSFFVSSEDIFDWTLSFNKLNNLAIIF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCEEEEEEEECCCCCEEEEE
GREDRGLTNSELLLANKTFSIPTSQNNPSLNLSHAVSIVLYELNKSSKKNLNNELKVFNL
ECCCCCCCCCEEEEEECEEECCCCCCCCCEEHHHHEEEEEEECCCCHHHCCCCCEEEEEE
ASSKEVHDTFLDIEEMLLRVGYLLKHTSKAKISKFKNFILRANTSMHEINVLRGIVHQIN
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
WFLNNSKKNK
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]