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The map label for this gene is vacB [H]

Identifier: 123968555

GI number: 123968555

Start: 881063

End: 883285

Strand: Direct

Name: vacB [H]

Synonym: A9601_10221

Alternate gene names: 123968555

Gene position: 881063-883285 (Clockwise)

Preceding gene: 123968554

Following gene: 123968556

Centisome position: 52.76

GC content: 26.68

Gene sequence:

>2223_bases
ATGTTCACATCATCTTCAATAATTGATAATCTTAATCAGTCAGAAGGGTTAGAATATAAAAAATTATGCAGATTATTAAA
AATAACAAAGAAATCTGATAAGGATAAATTAGATATTGCTTTAACAGCTCTAGAAAAACTTGAGATAATTAATAAAAATG
AAAATGATGAATATACTTGCATAAAGGATAATGATCATCTTGTCGCCAAAATAAGATGTAGTAGCAAAGGCTATTGCTTT
GCTGTAAGAGGAAAAGACAAGGAAGACATCTACATTAAAGAAAATCTACTTAACTATGCATGGAATGGAGATAAAGTTTT
AGTAAGGATAATAAAAGAGGGATATAGAAGAAGATCACCTGAAGGAATAGTTGATTGTATTCTTGAAAGATCAAATCAAA
TACTTCTTTCTAAAGTAGAAATAATAAACAATGATGTATATGCAATCCCAATAGACGATAGGATCCTTTCTAAAATTAAA
CTTCCAAAAGAAAATAAAAAATACATTTTCAATCCAGACAATAAGAATATAGTAAAAGTTGAGATTGATAGATTCCCAAT
AGGTCAAGAAGAAGGTCTAGGTCATGTGATACAAGAACTAAAACTAAATAATAATGAAGACTATGATACGGACTTTGTTT
TATCTAAAAGCAATATCGTCAAATCATACGATTTAAATCATATTGAATCAAAAAAAATAGAACAAAGGGAGAGAATAGAC
CTTTCAGATAAAAACTCTTATTTATTCAAAAGTTGGAATTCTAATAATTCTCCAATGCCTCCAATGATTCAAATAGAGCA
GGGGAAAAATAAAAATACTAAATTATGGATACATACAAATAATCTTGCAGAAAGAGTAGATCTAAATAGTAAAAAATCTC
TAGAAATATTATTCAAAGGCTTTGAATCATTACCCTTATTAAATGATTGGCAAAACTACCTTGGTGAAGCCATAAGAAAT
GATTCTGAATTTAAAATTGGTGAAAAAAATGAAGCAATAAGCCTCTGTATCAATTTAAATAGTGATAATGAAATAATTGA
TTGGACATTTCATCTTACTTTAGTAAGATGCACTCTTATTGTTGGAAGTGCTCATACTGACGCGCTTCTATCTAGAAAAA
GCAAATCAAGAATAACATCTCGGGTATTAAAACCTATAAAGGAATATGTCGACGATTTAGATAAAATACTAGAAATTTCA
TGTTCATTTAGAGAAAAACATCTTTTGGAGGGTAAGGTGGAAATTCCTGCGCCACTGAATAAGATTGAAGCACTAGAAGA
ATTTTTTATTCACAATCCTGCTGAATATTCAAAAGGATATTTTGAATCATTAAATAAAGAAGATTGCCAAACTTACCTTT
CACCAATACTTTATGAAGCTAATTTAATATGGTTCAAACATTCAAATCAATATGGATTAAAAAGTGCAGGATACATCTCA
AATGGAATAGATTACGTTAATGCTAATGAAATTATCAAATATTCAGAATTTATTGATAATGATTTAGAGCTTAATGAAGA
TGGCAATTTGACATTTAGCCAAGTAATTAAATTATGTGACGACGAAAATAAAAAAAGAATCTTACATAAACTTCTAATTA
ATGAATTTAAGGACAATGAAATAAGGTTGATATCTAAAGATGCTGATAATGATGAATCAGAAAAATTATTTATTTCTCCA
TGGACAATTCCTGGATATGACTTTTCAAATCTTATAAATCAGTACTGTATTTTTAATATGATAATAAATGGTAAGAAATC
AAAGAAAAATAATATAAATGAAATTAATATATCTGATAGTAATTCAATAGAATTAGTAAAATGGGATATATTTAATTCAT
CAATTTCAAAAAATCTAGAAATATTATTTAACAAGTTTGTGATAGATAAACTTAATGAATTTAAGTACAAAGTTAACCAA
TATAAATCTAATATGATAAATATAAAAAAAGTAAGAAAAGCAGAAAAATTACTAGGTAATATTTATAGTGGGTTTATTTT
ATCAGTGCAAACATATGGTTTCTTTGTTGAGATATCAGAACTAAATGTAGAGGGTTTAGTACACGTAAGCACACTTAATA
ATGATTGGTATGAATACAGGTCAAGACAAAATCTATTGATTGGAAGAAAATCCAAAAAATCATATAAAGTTGGTGATGCA
ATAGAAGTAAAAATTATAAAAGTCGATATTCTTAAATATCAAATTGATTTAGAATTAACATAA

Upstream 100 bases:

>100_bases
AGAAAAAGAAGAGAAAGAAAATAATTAGTTTTAAGCTTGGATAATAAGGTGTAATAATAAAGTATAACCCTCTAAATTAA
TTTATAATTACACTAAGATC

Downstream 100 bases:

>100_bases
ATAAATATTCACAAAATATATTATGGAAATATTAACCAAAAAAATTTTAAGATAACTTTTTATAATAATGTTTAAGAAAA
AATATTTACTTTTAACACTT

Product: putative acetazolamide conferring resistance protein Zam

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNENDEYTCIKDNDHLVAKIRCSSKGYCF
AVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIK
LPKENKKYIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELKLNNNEDYDTDFVLSKSNIVKSYDLNHIESKKIEQRERID
LSDKNSYLFKSWNSNNSPMPPMIQIEQGKNKNTKLWIHTNNLAERVDLNSKKSLEILFKGFESLPLLNDWQNYLGEAIRN
DSEFKIGEKNEAISLCINLNSDNEIIDWTFHLTLVRCTLIVGSAHTDALLSRKSKSRITSRVLKPIKEYVDDLDKILEIS
CSFREKHLLEGKVEIPAPLNKIEALEEFFIHNPAEYSKGYFESLNKEDCQTYLSPILYEANLIWFKHSNQYGLKSAGYIS
NGIDYVNANEIIKYSEFIDNDLELNEDGNLTFSQVIKLCDDENKKRILHKLLINEFKDNEIRLISKDADNDESEKLFISP
WTIPGYDFSNLINQYCIFNMIINGKKSKKNNINEINISDSNSIELVKWDIFNSSISKNLEILFNKFVIDKLNEFKYKVNQ
YKSNMINIKKVRKAEKLLGNIYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLIGRKSKKSYKVGDA
IEVKIIKVDILKYQIDLELT

Sequences:

>Translated_740_residues
MFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNENDEYTCIKDNDHLVAKIRCSSKGYCF
AVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIK
LPKENKKYIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELKLNNNEDYDTDFVLSKSNIVKSYDLNHIESKKIEQRERID
LSDKNSYLFKSWNSNNSPMPPMIQIEQGKNKNTKLWIHTNNLAERVDLNSKKSLEILFKGFESLPLLNDWQNYLGEAIRN
DSEFKIGEKNEAISLCINLNSDNEIIDWTFHLTLVRCTLIVGSAHTDALLSRKSKSRITSRVLKPIKEYVDDLDKILEIS
CSFREKHLLEGKVEIPAPLNKIEALEEFFIHNPAEYSKGYFESLNKEDCQTYLSPILYEANLIWFKHSNQYGLKSAGYIS
NGIDYVNANEIIKYSEFIDNDLELNEDGNLTFSQVIKLCDDENKKRILHKLLINEFKDNEIRLISKDADNDESEKLFISP
WTIPGYDFSNLINQYCIFNMIINGKKSKKNNINEINISDSNSIELVKWDIFNSSISKNLEILFNKFVIDKLNEFKYKVNQ
YKSNMINIKKVRKAEKLLGNIYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLIGRKSKKSYKVGDA
IEVKIIKVDILKYQIDLELT
>Mature_740_residues
MFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNENDEYTCIKDNDHLVAKIRCSSKGYCF
AVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSPEGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIK
LPKENKKYIFNPDNKNIVKVEIDRFPIGQEEGLGHVIQELKLNNNEDYDTDFVLSKSNIVKSYDLNHIESKKIEQRERID
LSDKNSYLFKSWNSNNSPMPPMIQIEQGKNKNTKLWIHTNNLAERVDLNSKKSLEILFKGFESLPLLNDWQNYLGEAIRN
DSEFKIGEKNEAISLCINLNSDNEIIDWTFHLTLVRCTLIVGSAHTDALLSRKSKSRITSRVLKPIKEYVDDLDKILEIS
CSFREKHLLEGKVEIPAPLNKIEALEEFFIHNPAEYSKGYFESLNKEDCQTYLSPILYEANLIWFKHSNQYGLKSAGYIS
NGIDYVNANEIIKYSEFIDNDLELNEDGNLTFSQVIKLCDDENKKRILHKLLINEFKDNEIRLISKDADNDESEKLFISP
WTIPGYDFSNLINQYCIFNMIINGKKSKKNNINEINISDSNSIELVKWDIFNSSISKNLEILFNKFVIDKLNEFKYKVNQ
YKSNMINIKKVRKAEKLLGNIYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLIGRKSKKSYKVGDA
IEVKIIKVDILKYQIDLELT

Specific function: Not known; control resistance to the carbonic anhydrase inhibitor acetazolamide [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082383, Length=82, Percent_Identity=40.2439024390244, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 85962; Mature: 85962

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50126 S1 ; PS00591 GLYCOSYL_HYDROL_F10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNENDEYTC
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
IKDNDHLVAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSP
EECCCEEEEEEEECCCCCEEEECCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHCCCC
EGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIKLPKENKKYIFNPDNKNIVKV
HHHHHHHHHCCCCEEEEEEEECCCCEEEEECCHHHHHHCCCCCCCCCEEECCCCCCEEEE
EIDRFPIGQEEGLGHVIQELKLNNNEDYDTDFVLSKSNIVKSYDLNHIESKKIEQRERID
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHCCC
LSDKNSYLFKSWNSNNSPMPPMIQIEQGKNKNTKLWIHTNNLAERVDLNSKKSLEILFKG
CCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHC
FESLPLLNDWQNYLGEAIRNDSEFKIGEKNEAISLCINLNSDNEIIDWTFHLTLVRCTLI
HHCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCEEEEEHHHHHHEEHHH
VGSAHTDALLSRKSKSRITSRVLKPIKEYVDDLDKILEISCSFREKHLLEGKVEIPAPLN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEECCCCHH
KIEALEEFFIHNPAEYSKGYFESLNKEDCQTYLSPILYEANLIWFKHSNQYGLKSAGYIS
HHHHHHHHHCCCCHHHCCHHHHCCCHHHHHHHHHHHHEECEEEEEEECCCCCCCCCCCCC
NGIDYVNANEIIKYSEFIDNDLELNEDGNLTFSQVIKLCDDENKKRILHKLLINEFKDNE
CCCCCCCHHHHHHHHHHCCCCCEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
IRLISKDADNDESEKLFISPWTIPGYDFSNLINQYCIFNMIINGKKSKKNNINEINISDS
EEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCEEEECCC
NSIELVKWDIFNSSISKNLEILFNKFVIDKLNEFKYKVNQYKSNMINIKKVRKAEKLLGN
CCEEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLIGRKSKKSYKVGDA
HHHCCEEEEEEEEEEEEEEEECCCEEEEEEECCCHHHHHHCCCCEEEECCCCCCCCCCCE
IEVKIIKVDILKYQIDLELT
EEEEEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MFTSSSIIDNLNQSEGLEYKKLCRLLKITKKSDKDKLDIALTALEKLEIINKNENDEYTC
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
IKDNDHLVAKIRCSSKGYCFAVRGKDKEDIYIKENLLNYAWNGDKVLVRIIKEGYRRRSP
EECCCEEEEEEEECCCCCEEEECCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHCCCC
EGIVDCILERSNQILLSKVEIINNDVYAIPIDDRILSKIKLPKENKKYIFNPDNKNIVKV
HHHHHHHHHCCCCEEEEEEEECCCCEEEEECCHHHHHHCCCCCCCCCEEECCCCCCEEEE
EIDRFPIGQEEGLGHVIQELKLNNNEDYDTDFVLSKSNIVKSYDLNHIESKKIEQRERID
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHCCC
LSDKNSYLFKSWNSNNSPMPPMIQIEQGKNKNTKLWIHTNNLAERVDLNSKKSLEILFKG
CCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHC
FESLPLLNDWQNYLGEAIRNDSEFKIGEKNEAISLCINLNSDNEIIDWTFHLTLVRCTLI
HHCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCEEEEEHHHHHHEEHHH
VGSAHTDALLSRKSKSRITSRVLKPIKEYVDDLDKILEISCSFREKHLLEGKVEIPAPLN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEECCCCHH
KIEALEEFFIHNPAEYSKGYFESLNKEDCQTYLSPILYEANLIWFKHSNQYGLKSAGYIS
HHHHHHHHHCCCCHHHCCHHHHCCCHHHHHHHHHHHHEECEEEEEEECCCCCCCCCCCCC
NGIDYVNANEIIKYSEFIDNDLELNEDGNLTFSQVIKLCDDENKKRILHKLLINEFKDNE
CCCCCCCHHHHHHHHHHCCCCCEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
IRLISKDADNDESEKLFISPWTIPGYDFSNLINQYCIFNMIINGKKSKKNNINEINISDS
EEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCEEEECCC
NSIELVKWDIFNSSISKNLEILFNKFVIDKLNEFKYKVNQYKSNMINIKKVRKAEKLLGN
CCEEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IYSGFILSVQTYGFFVEISELNVEGLVHVSTLNNDWYEYRSRQNLLIGRKSKKSYKVGDA
HHHCCEEEEEEEEEEEEEEEECCCEEEEEEECCCHHHHHHCCCCEEEECCCCCCCCCCCE
IEVKIIKVDILKYQIDLELT
EEEEEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7727754; 8905231 [H]