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The map label for this gene is cspR [H]

Identifier: 123968533

GI number: 123968533

Start: 870267

End: 870749

Strand: Direct

Name: cspR [H]

Synonym: A9601_10001

Alternate gene names: 123968533

Gene position: 870267-870749 (Clockwise)

Preceding gene: 123968532

Following gene: 123968534

Centisome position: 52.12

GC content: 29.61

Gene sequence:

>483_bases
TTGGAAGTCGCTCTTTTTGAACCTAGAATCCCACAAAATACTGGTAATATTGCCAGATCATGTGCTGCATTTAATATACC
TTTAAATCTTATAGAGCCCTTAGGTTTTAAACTAGAAGATAAATATTTAAAAAGAGCAGGTTTAGACTATTGGCCTTTAG
TTACTGTTAATCAGTATGAGAATTTTGAAAAATTTTTAGCGTCAAAATCAACAAAAAGAATAATCTCTTTTAGTAAAAAA
AATGGATTATATTTGAAGGATTTTAAATTTAAGCAAGATGATATTTTGCTATTTGGGAGAGAAGATTCAGGATTACCCGA
TTCCATTATTGATAAAAGCGACTTTTTAATATCAATATTTATGCCGAATATACAGACTGGTAACAATGATCAAAAAGGTG
TGAGAAGTCTAAACCTTTCTGTTGCATGCGGAATTGCTATATATGAGGCCTACAAACAAATAAATTTTCAAAATGGTAAT
TAA

Upstream 100 bases:

>100_bases
TTCAAAAGAGAATAACCAAAATATCAAAAGATAATTGGCTGGCCATAAACGGCAGAGCAATCAAAATAGATGAAATAAGT
ATTGAAATACCTACTTAAAA

Downstream 100 bases:

>100_bases
GTACAACCAATGCCTTACAATATTCCCATGTCTCGGTAGCTCAGCTGGTTAGAGCGGCGGATTCATAGCCCGCAGGTCGC
GTGTTCAAGTCACGCTCGAG

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MEVALFEPRIPQNTGNIARSCAAFNIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNQYENFEKFLASKSTKRIISFSKK
NGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIFMPNIQTGNNDQKGVRSLNLSVACGIAIYEAYKQINFQNGN

Sequences:

>Translated_160_residues
MEVALFEPRIPQNTGNIARSCAAFNIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNQYENFEKFLASKSTKRIISFSKK
NGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIFMPNIQTGNNDQKGVRSLNLSVACGIAIYEAYKQINFQNGN
>Mature_160_residues
MEVALFEPRIPQNTGNIARSCAAFNIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNQYENFEKFLASKSTKRIISFSKK
NGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIFMPNIQTGNNDQKGVRSLNLSVACGIAIYEAYKQINFQNGN

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=35.8974358974359, Blast_Score=114, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18197; Mature: 18197

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVALFEPRIPQNTGNIARSCAAFNIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNQYE
CCEEEECCCCCCCCCHHHHHHEEECCCHHHHCCCCCCCCHHHHHHCCCCCCCEEEEHHHH
NFEKFLASKSTKRIISFSKKNGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIF
HHHHHHHCCCHHHHEEECCCCCEEEEECEECCCCEEEEECCCCCCCHHHCCCCCEEEEEE
MPNIQTGNNDQKGVRSLNLSVACGIAIYEAYKQINFQNGN
ECCCCCCCCCCCHHHEECHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEVALFEPRIPQNTGNIARSCAAFNIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNQYE
CCEEEECCCCCCCCCHHHHHHEEECCCHHHHCCCCCCCCHHHHHHCCCCCCCEEEEHHHH
NFEKFLASKSTKRIISFSKKNGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIF
HHHHHHHCCCHHHHEEECCCCCEEEEECEECCCCEEEEECCCCCCCHHHCCCCCEEEEEE
MPNIQTGNNDQKGVRSLNLSVACGIAIYEAYKQINFQNGN
ECCCCCCCCCCCHHHEECHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA