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The map label for this gene is pykF [H]

Identifier: 123968482

GI number: 123968482

Start: 817097

End: 818887

Strand: Reverse

Name: pykF [H]

Synonym: A9601_09491

Alternate gene names: 123968482

Gene position: 818887-817097 (Counterclockwise)

Preceding gene: 123968484

Following gene: 123968481

Centisome position: 49.04

GC content: 34.23

Gene sequence:

>1791_bases
ATGTCGAATATTGATTTAAAAAGAAGAACAAAAATAGTAGCAACTATTGGCCCTGCGACCCAATCTGAAGAGATAATTAC
AAATTTAATTAAAGCTGGAGTAACAACATTCAGATTAAATTTCTCACATGGAGATCATAAAGATCATGCTGAGAGAATAA
AAACCATAAGGGAAGTATCAAAAAAGTTAGATATAGATATTGGAATATTGCAAGATCTTCAAGGACCTAAAATACGATTA
GGACGCTTTAAAGATGGGCCAGTAAAAGTTAAAAAAGGCGATAAATTTACGCTTACATCAAATGAAGTCGAATGTACAAA
TACTATTGCAAATGTTACCTACGACAAACTTTCTCAAGAAGTTAGCGAAGGGAAAAGAATACTTTTAGATGATGGAAAAA
TAGAAATGATTGTAGAAAAAGTTGATACAAAAGCTAATAATCTGGAGTGCATGGTAACTGTAGGAGGGGTTCTTTCAAAC
AATAAAGGTGTTAATTTTCCAGATGTTCAATTATCAGTAAAAGCATTAACAGAAAAGGATAAAGAGGATTTAAAATTTGG
TTTATCTGTAGGAGTTGATTGGATAGCACTAAGTTTCGTAAGAAATCCATCCGATATAAATGAGATAAAAGATTTAATAA
ACACAAATGGGCATTCAACTCCGGTAGTCGCAAAAATAGAAAAATTTGAAGCAATTGATCAGATCGATACAGTTTTACCC
TTATGTGATGGGGTTATGGTTGCAAGAGGTGATTTGGGAGTAGAAATGCCTGCTGAAGAAGTTCCTCTTCTACAAAAGGA
GTTAATAAGAAAAGCTAATTCATTAGGTATCCCAATAATTACAGCGACTCAAATGCTTGATTCTATGGCTTCTAATCCAA
GACCAACTAGGGCCGAAGTTAGTGATGTTGCAAATGCAATTCTGGATGGTACAGATGCTGTAATGCTTTCAAACGAAACT
GCAGTTGGCGATTATCCTGTAGAGGCAGTTGAAACGATGGCAACCATAGCAAGAAGAATTGAAAGGGATTATCCACTTAA
GGCTATTGAAAGCCACTTACCCAGTACGATCCCAAATGCTATTAGTGCAGCAGTAAGCAATATAGCTAGACAACTTGATG
CAGGAGCTATAATCCCTTTAACTAAATCAGGTTCTACCGCTCGAAATGTAAGTAAGTTCAGACCTCCAACACCTATCTTG
GCAACTACTACAGAAAGAAGTGTAGCGAGAAGATTGCAACTTGTTTGGGGAGTTACCCCAATAGTAGTTAAAAATGATGA
AAGAACAGCAAAGACTTTTAGTTTAGCTATGCAAATTGCTCAAGAGATGGGCATCCTAAATCAAGGAGATTTAGTAGTTC
AAACCGCAGGTACATTAACAGGAATTAGCGGCTCTACAGATTTAATAAAAGTCGGTTTAGTAAGAAAGATTGTATCAAGA
GGAATTTCAATAGGGGAAATCGGTGTTACAGGTAAAGCAAGGATAATTAAAAATAATCTTGATATATCCTTAATTTGCCC
AGGAGAAATATTATTTGTTCCCAAGGAATTAATGAAAAATATTCCACTGAGTAAAAATATTGCCGGCATTGTTACAAACC
AAAATGTAAATGATGTTTACGCCTTTTTTAATAAAAATAATAAAAAGATTTCTACAATTTGTAATTTAGAGAATATGGAT
AATCATCAAATCAGTAATGGCGATCTTATTACCCTCCAGCTTAATGAAGGTGTTATATACATGGGACAAATTGAAGATGA
TGATGCAATAGATAAATATAAATATGTCTAG

Upstream 100 bases:

>100_bases
TGTTTCTCTAGCTTTTTTCTGATAAGTTTTAAAATCCATGACTTAAATAACTAACTTTGGGTATGGTAAATTTATTTATA
TCTAGCAATATTATCTATAT

Downstream 100 bases:

>100_bases
GAATATTTCAATAAAAGAAGCCTTAGGCATGGCAACAAAAACTTTAGTTTCGAACAAATTGAGAAGTTCGCTAACAATGC
TTGGAATAATTATAGGAAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHKDHAERIKTIREVSKKLDIDIGILQDLQGPKIRL
GRFKDGPVKVKKGDKFTLTSNEVECTNTIANVTYDKLSQEVSEGKRILLDDGKIEMIVEKVDTKANNLECMVTVGGVLSN
NKGVNFPDVQLSVKALTEKDKEDLKFGLSVGVDWIALSFVRNPSDINEIKDLINTNGHSTPVVAKIEKFEAIDQIDTVLP
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANSLGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPVEAVETMATIARRIERDYPLKAIESHLPSTIPNAISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPIL
ATTTERSVARRLQLVWGVTPIVVKNDERTAKTFSLAMQIAQEMGILNQGDLVVQTAGTLTGISGSTDLIKVGLVRKIVSR
GISIGEIGVTGKARIIKNNLDISLICPGEILFVPKELMKNIPLSKNIAGIVTNQNVNDVYAFFNKNNKKISTICNLENMD
NHQISNGDLITLQLNEGVIYMGQIEDDDAIDKYKYV

Sequences:

>Translated_596_residues
MSNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHKDHAERIKTIREVSKKLDIDIGILQDLQGPKIRL
GRFKDGPVKVKKGDKFTLTSNEVECTNTIANVTYDKLSQEVSEGKRILLDDGKIEMIVEKVDTKANNLECMVTVGGVLSN
NKGVNFPDVQLSVKALTEKDKEDLKFGLSVGVDWIALSFVRNPSDINEIKDLINTNGHSTPVVAKIEKFEAIDQIDTVLP
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANSLGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPVEAVETMATIARRIERDYPLKAIESHLPSTIPNAISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPIL
ATTTERSVARRLQLVWGVTPIVVKNDERTAKTFSLAMQIAQEMGILNQGDLVVQTAGTLTGISGSTDLIKVGLVRKIVSR
GISIGEIGVTGKARIIKNNLDISLICPGEILFVPKELMKNIPLSKNIAGIVTNQNVNDVYAFFNKNNKKISTICNLENMD
NHQISNGDLITLQLNEGVIYMGQIEDDDAIDKYKYV
>Mature_595_residues
SNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHKDHAERIKTIREVSKKLDIDIGILQDLQGPKIRLG
RFKDGPVKVKKGDKFTLTSNEVECTNTIANVTYDKLSQEVSEGKRILLDDGKIEMIVEKVDTKANNLECMVTVGGVLSNN
KGVNFPDVQLSVKALTEKDKEDLKFGLSVGVDWIALSFVRNPSDINEIKDLINTNGHSTPVVAKIEKFEAIDQIDTVLPL
CDGVMVARGDLGVEMPAEEVPLLQKELIRKANSLGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNETA
VGDYPVEAVETMATIARRIERDYPLKAIESHLPSTIPNAISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILA
TTTERSVARRLQLVWGVTPIVVKNDERTAKTFSLAMQIAQEMGILNQGDLVVQTAGTLTGISGSTDLIKVGLVRKIVSRG
ISIGEIGVTGKARIIKNNLDISLICPGEILFVPKELMKNIPLSKNIAGIVTNQNVNDVYAFFNKNNKKISTICNLENMDN
HQISNGDLITLQLNEGVIYMGQIEDDDAIDKYKYV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=39.7540983606557, Blast_Score=328, Evalue=1e-89,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=39.1836734693878, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=39.1836734693878, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=39.1393442622951, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=39.1393442622951, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=39.7923875432526, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=39.7923875432526, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=36.7088607594937, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=36.7088607594937, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=35.6481481481481, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787965, Length=469, Percent_Identity=43.7100213219616, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788160, Length=473, Percent_Identity=41.8604651162791, Blast_Score=322, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=38.4615384615385, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=39.6303901437372, Blast_Score=300, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=39.6303901437372, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=39.6303901437372, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=39.6303901437372, Blast_Score=298, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=39.0946502057613, Blast_Score=297, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6324923, Length=478, Percent_Identity=37.6569037656904, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=38.6965376782077, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=38.6965376782077, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=39.9014778325123, Blast_Score=286, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=33.1275720164609, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=40, Blast_Score=189, Evalue=6e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 64982; Mature: 64851

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHKDHAERIKTIREVS
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHH
KKLDIDIGILQDLQGPKIRLGRFKDGPVKVKKGDKFTLTSNEVECTNTIANVTYDKLSQE
HHCCCCEEHHHCCCCCCEEECCCCCCCEEEECCCEEEEECCCCEECHHHHHHHHHHHHHH
VSEGKRILLDDGKIEMIVEKVDTKANNLECMVTVGGVLSNNKGVNFPDVQLSVKALTEKD
HHCCCEEEEECCCEEEEEEHHCCCCCCEEEEEEECCEEECCCCCCCCCCEEEEHHHHCCC
KEDLKFGLSVGVDWIALSFVRNPSDINEIKDLINTNGHSTPVVAKIEKFEAIDQIDTVLP
HHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANSLGIPIITATQMLDSMASNPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPVEAVETMATIARRIERDYPLKAIESHLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHH
ISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILATTTERSVARRLQLVWGVTP
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCE
IVVKNDERTAKTFSLAMQIAQEMGILNQGDLVVQTAGTLTGISGSTDLIKVGLVRKIVSR
EEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHC
GISIGEIGVTGKARIIKNNLDISLICPGEILFVPKELMKNIPLSKNIAGIVTNQNVNDVY
CCCEEEECCCCCEEEEECCCCEEEECCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCEE
AFFNKNNKKISTICNLENMDNHQISNGDLITLQLNEGVIYMGQIEDDDAIDKYKYV
EEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHCCC
>Mature Secondary Structure 
SNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHKDHAERIKTIREVS
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHH
KKLDIDIGILQDLQGPKIRLGRFKDGPVKVKKGDKFTLTSNEVECTNTIANVTYDKLSQE
HHCCCCEEHHHCCCCCCEEECCCCCCCEEEECCCEEEEECCCCEECHHHHHHHHHHHHHH
VSEGKRILLDDGKIEMIVEKVDTKANNLECMVTVGGVLSNNKGVNFPDVQLSVKALTEKD
HHCCCEEEEECCCEEEEEEHHCCCCCCEEEEEEECCEEECCCCCCCCCCEEEEHHHHCCC
KEDLKFGLSVGVDWIALSFVRNPSDINEIKDLINTNGHSTPVVAKIEKFEAIDQIDTVLP
HHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKELIRKANSLGIPIITATQMLDSMASNPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPVEAVETMATIARRIERDYPLKAIESHLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHH
ISAAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILATTTERSVARRLQLVWGVTP
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCE
IVVKNDERTAKTFSLAMQIAQEMGILNQGDLVVQTAGTLTGISGSTDLIKVGLVRKIVSR
EEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHC
GISIGEIGVTGKARIIKNNLDISLICPGEILFVPKELMKNIPLSKNIAGIVTNQNVNDVY
CCCEEEECCCCCEEEEECCCCEEEECCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCEE
AFFNKNNKKISTICNLENMDNHQISNGDLITLQLNEGVIYMGQIEDDDAIDKYKYV
EEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]