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The map label for this gene is gatA [H]

Identifier: 123968447

GI number: 123968447

Start: 784433

End: 785881

Strand: Direct

Name: gatA [H]

Synonym: A9601_09141

Alternate gene names: 123968447

Gene position: 784433-785881 (Clockwise)

Preceding gene: 123968446

Following gene: 123968448

Centisome position: 46.98

GC content: 36.16

Gene sequence:

>1449_bases
ATGAACTTTAATTCTTTAAGAAAAGAAATTACTAGCAATAATGCTTCTGTTAAGGAATTAGTTAATGATTTTTTTGTAAA
AATCGATCATAAGGACTCTAAGATTAATTCATATATTTGTACTACAAAAGATAATGCTATCGCGCAAGCAGAGGATATTG
ATAAATTAATTCAAAATAATGAAAAACTTCCTCCTCTCGCAGGGATGCCAATAGCAATAAAGGATAATATTTGCACCAAA
GGAGTTGCAACAACTTGTGCGAGTAATATGCTCAAAAACTTTGTTGCACCTTATGAATCCACAGCTTCTAGTAAATTATG
GTCTTCAGGGGGAATTTGTTTAGGAAAAACAAATTTAGACGAATTTGCAATGGGTAGTTCAACGGAAACTTCTGTATTTG
GAGTTACTTCAAATCCTTGGGATATTAATAGAGTGCCAGGAGGTAGTTCAGGCGGTAGTGCTGCTTCAGTTGCCGCTGGA
TTTTGTGCAGCTGCTATAGGTTCTGATACAGGAGGATCAATAAGGCAACCAGCTTCTTTTTGTGGCGTCGTAGGTCTTAA
ACCTACTTATGGCAGAGTTAGCAGATGGGGACTTGTAGCATTTGCTAGCTCTCTTGATCAAATTGGTCCAATTACAAATA
CTGTTTCAGATGCAGCTGAAATTCTTCATTCAATATCTGGTAAAGACCCCTTTGACTCAACATGTCTTGATAAGCCAGTA
CCAAATTATTTGACTGACCTAAATAAATCTATAAAGGGCTTAAAAATTGGAATCATTAAAGAGTGTTTTGAACACCCAGG
GCTTAATCCAGAAGTTAAGGAATCTGTTCTTTCTGGAGTTGATAGGTTCCAAGCTTTAGGTGCTGAAATTATCGAAGTTG
AATGTCCTAGATTTAACGATGGAATTGCGACATATTATGTCATTGCACCATCTGAAGCCTCCGCAAATTTAGCTAGATAT
GATGGCGTTAAATATGGCTACAGATCGAATGAGGGTTCAAATCTTATAGATATGACTTCAAAAAGTAGAGCTGAAGGTTT
TGGAGATGAGGTACAGAGAAGAATTTTGATAGGTACTTATGCTTTGTCAGCCGGATACAGTGATGCCTATTACAAGAAGG
CACAAAAAGTAAGGACGCTTATAAGAAAAGATTTTGATAATGCGTTTAAGAAAGTAGATGTTTTATTAACTCCAACCTGC
CCAACCACTGCTTTTTTGAAGGGAGATTTTGTCAATGATCCACTTTCAATGTATTTATCTGATCTATTAACTGTTCCCGC
TAATTTGGCAGGCCTCCCAGCAATCAGTATTCCTTGTGGTTTTGATACTAAAGGATTACCTATAGGACTGCAATTAATAG
GCAATGTACTAGAGGAAGATAGAATATTGAATGCCGCAAATATCTTTGAAATTGATGCTCATGTAATTAAGAAAAGACCT
TTATTTTAA

Upstream 100 bases:

>100_bases
TGATGAAGGGTCTAAAAACATTAAACATAGTTTAGTTCGCTGCAAAAAAGAAGAACCACTTACTGTTTGATAATTTTTAA
TTTAAGAAATAAATCAAGGA

Downstream 100 bases:

>100_bases
ATTTGTATTAATAATTAATTTGTAATCACAAATTATAGATCTTTTAGAAATCTTCTCTATCTTATATATATAAATTATTT
GAATATGGCTTTCGTTCCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNFNSLRKEITSNNASVKELVNDFFVKIDHKDSKINSYICTTKDNAIAQAEDIDKLIQNNEKLPPLAGMPIAIKDNICTK
GVATTCASNMLKNFVAPYESTASSKLWSSGGICLGKTNLDEFAMGSSTETSVFGVTSNPWDINRVPGGSSGGSAASVAAG
FCAAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILHSISGKDPFDSTCLDKPV
PNYLTDLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGYRSNEGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRKDFDNAFKKVDVLLTPTC
PTTAFLKGDFVNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDRILNAANIFEIDAHVIKKRP
LF

Sequences:

>Translated_482_residues
MNFNSLRKEITSNNASVKELVNDFFVKIDHKDSKINSYICTTKDNAIAQAEDIDKLIQNNEKLPPLAGMPIAIKDNICTK
GVATTCASNMLKNFVAPYESTASSKLWSSGGICLGKTNLDEFAMGSSTETSVFGVTSNPWDINRVPGGSSGGSAASVAAG
FCAAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILHSISGKDPFDSTCLDKPV
PNYLTDLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGYRSNEGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRKDFDNAFKKVDVLLTPTC
PTTAFLKGDFVNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDRILNAANIFEIDAHVIKKRP
LF
>Mature_482_residues
MNFNSLRKEITSNNASVKELVNDFFVKIDHKDSKINSYICTTKDNAIAQAEDIDKLIQNNEKLPPLAGMPIAIKDNICTK
GVATTCASNMLKNFVAPYESTASSKLWSSGGICLGKTNLDEFAMGSSTETSVFGVTSNPWDINRVPGGSSGGSAASVAAG
FCAAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILHSISGKDPFDSTCLDKPV
PNYLTDLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGYRSNEGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRKDFDNAFKKVDVLLTPTC
PTTAFLKGDFVNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDRILNAANIFEIDAHVIKKRP
LF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=464, Percent_Identity=42.2413793103448, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=23.6842105263158, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI166795287, Length=231, Percent_Identity=29.004329004329, Blast_Score=72, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=417, Percent_Identity=38.6091127098321, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=480, Percent_Identity=25.8333333333333, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71990152, Length=303, Percent_Identity=31.023102310231, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=316, Percent_Identity=28.4810126582279, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=240, Percent_Identity=32.9166666666667, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17538254, Length=319, Percent_Identity=25.0783699059561, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=311, Percent_Identity=29.5819935691318, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=344, Percent_Identity=27.3255813953488, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=422, Percent_Identity=36.9668246445498, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=32.3595505617978, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=193, Percent_Identity=31.6062176165803, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=452, Percent_Identity=43.5840707964602, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24652985, Length=481, Percent_Identity=25.1559251559252, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922090, Length=481, Percent_Identity=25.1559251559252, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652981, Length=481, Percent_Identity=25.1559251559252, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652983, Length=481, Percent_Identity=25.1559251559252, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24644968, Length=489, Percent_Identity=26.5848670756646, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078093, Length=483, Percent_Identity=28.1573498964803, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45550774, Length=475, Percent_Identity=24.6315789473684, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=24.4897959183673, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=24.4897959183673, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648441, Length=432, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648439, Length=432, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356731, Length=470, Percent_Identity=24.2553191489362, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51738; Mature: 51738

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNSLRKEITSNNASVKELVNDFFVKIDHKDSKINSYICTTKDNAIAQAEDIDKLIQNN
CCHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
EKLPPLAGMPIAIKDNICTKGVATTCASNMLKNFVAPYESTASSKLWSSGGICLGKTNLD
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCH
EFAMGSSTETSVFGVTSNPWDINRVPGGSSGGSAASVAAGFCAAAIGSDTGGSIRQPASF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILHSISGKDPFDSTCLDKPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PNYLTDLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFND
CHHHHHHHHHHCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRSNEGSNLIDMTSKSRAEGFGDEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHEEEH
ALSAGYSDAYYKKAQKVRTLIRKDFDNAFKKVDVLLTPTCPTTAFLKGDFVNDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDRILNAANIFEIDAHVIKKRP
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEHHHHHHHCCC
LF
CC
>Mature Secondary Structure
MNFNSLRKEITSNNASVKELVNDFFVKIDHKDSKINSYICTTKDNAIAQAEDIDKLIQNN
CCHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
EKLPPLAGMPIAIKDNICTKGVATTCASNMLKNFVAPYESTASSKLWSSGGICLGKTNLD
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCH
EFAMGSSTETSVFGVTSNPWDINRVPGGSSGGSAASVAAGFCAAAIGSDTGGSIRQPASF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILHSISGKDPFDSTCLDKPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PNYLTDLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFND
CHHHHHHHHHHCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRSNEGSNLIDMTSKSRAEGFGDEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHEEEH
ALSAGYSDAYYKKAQKVRTLIRKDFDNAFKKVDVLLTPTCPTTAFLKGDFVNDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDRILNAANIFEIDAHVIKKRP
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEHHHHHHHCCC
LF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA