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The map label for this gene is carA [H]

Identifier: 123968442

GI number: 123968442

Start: 781067

End: 782206

Strand: Direct

Name: carA [H]

Synonym: A9601_09091

Alternate gene names: 123968442

Gene position: 781067-782206 (Clockwise)

Preceding gene: 123968441

Following gene: 123968444

Centisome position: 46.77

GC content: 32.11

Gene sequence:

>1140_bases
ATGATTAATCCATATAAGAAAAACGCGAAATTAGTTTTAAGTAATGGTATTGTATTTCCGGGATTCTTTTTTGGTGCTTC
TGGTACTGCAGTTGGTGAAATAGTTTTTAATACGGGAATGACCGGATATCAGGAAGTTATTACTGATCCTAGTTATTATG
GACAAATTTTAACGTTTACTTATCCAGAGATTGGAAATACTGGTATTAATTTTGAAGATTCAGAATCCAATATTAATATC
AAAGGTATAATTGTTAGAAATTTTTCATCTAATAACAGCAATTGGAGATCTAAAAAGAATTTTAATCAATGGTTAGTGGA
GAAAAATATCATTGGTCTATACGGAATTGATACAAGGGCTCTTGTTAAGATTTTAAGATCTACTGGCGCAATGAATGGAG
TTATTACCTCTCTAGATAAAACTGATGAAAGTTGTTTAAAGATAATTCAGGATACACCAAAGATGGAGGGATTAAATTTA
TCAAAAGTTGTTTCAACAAAGCAACAATATTTATGGAATAGTCATACACAAACAGTTTTTGATTTAAGAAAAAGATATGA
TGAATCTTCTAAAAAGTTAAAAATAGTGGCAATTGATTTTGGAATTAAAAATTCAATTTTAAATAGACTCGTATCCCATG
GTTGTGAAGTTTTGGTTTTACCTTCTCGATCTTCTCTAAATGATGTCCTGTCTAACAAGCCGGATGGTATATTTTTCTCA
AATGGTCCAGGCGATCCTTCTACTGTTTCTGAAGGTATAGACCTAGCAAAATCACTCATAGAATATGGTGAAATTCCTAT
GTTTGGTATATGCCTTGGCCACCAAATATTTGGATTAGCACTAGGTGGCTCAACTTATAAATTATCTTTTGGCCATCGTG
GTTTAAATCACCCTTGTGGTCAAAATAACAAGATAGAGATAACTAGTCAGAACCACGGTTTTGCTATTGATCCTAATTCT
CTACCAAAGAATTTAGTTAGAATAACCCATTACAACCTTAACGATAATACTGTGGCTGGCCTAGAAGTTAATAATAAGCC
AATATTTAGTGTGCAATATCATCCAGAAGCAGGACCTGGCCCACATGATTCAGATTATTTATTTAAAAAATTTGTTTCTC
TAATGTTAGAAAGATGTTGA

Upstream 100 bases:

>100_bases
GATTTAATAAAGCTTTATTTTCTATAAATCAAGGAGATCCTTGGAAAAAGTTTTTACTTTTAAAAAATTATTTACAAACA
AATTAATTAGTTTCAAATTG

Downstream 100 bases:

>100_bases
CATATTGTTTTCTTTTGATTTGATAATTACATTTGATACATTAATTTTTTGGAGGGATTAGATCATAGAAGATTTCCAGA
AGTTAACGGTTTCTTTAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MINPYKKNAKLVLSNGIVFPGFFFGASGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFTYPEIGNTGINFEDSESNINI
KGIIVRNFSSNNSNWRSKKNFNQWLVEKNIIGLYGIDTRALVKILRSTGAMNGVITSLDKTDESCLKIIQDTPKMEGLNL
SKVVSTKQQYLWNSHTQTVFDLRKRYDESSKKLKIVAIDFGIKNSILNRLVSHGCEVLVLPSRSSLNDVLSNKPDGIFFS
NGPGDPSTVSEGIDLAKSLIEYGEIPMFGICLGHQIFGLALGGSTYKLSFGHRGLNHPCGQNNKIEITSQNHGFAIDPNS
LPKNLVRITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPGPHDSDYLFKKFVSLMLERC

Sequences:

>Translated_379_residues
MINPYKKNAKLVLSNGIVFPGFFFGASGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFTYPEIGNTGINFEDSESNINI
KGIIVRNFSSNNSNWRSKKNFNQWLVEKNIIGLYGIDTRALVKILRSTGAMNGVITSLDKTDESCLKIIQDTPKMEGLNL
SKVVSTKQQYLWNSHTQTVFDLRKRYDESSKKLKIVAIDFGIKNSILNRLVSHGCEVLVLPSRSSLNDVLSNKPDGIFFS
NGPGDPSTVSEGIDLAKSLIEYGEIPMFGICLGHQIFGLALGGSTYKLSFGHRGLNHPCGQNNKIEITSQNHGFAIDPNS
LPKNLVRITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPGPHDSDYLFKKFVSLMLERC
>Mature_379_residues
MINPYKKNAKLVLSNGIVFPGFFFGASGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFTYPEIGNTGINFEDSESNINI
KGIIVRNFSSNNSNWRSKKNFNQWLVEKNIIGLYGIDTRALVKILRSTGAMNGVITSLDKTDESCLKIIQDTPKMEGLNL
SKVVSTKQQYLWNSHTQTVFDLRKRYDESSKKLKIVAIDFGIKNSILNRLVSHGCEVLVLPSRSSLNDVLSNKPDGIFFS
NGPGDPSTVSEGIDLAKSLIEYGEIPMFGICLGHQIFGLALGGSTYKLSFGHRGLNHPCGQNNKIEITSQNHGFAIDPNS
LPKNLVRITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPGPHDSDYLFKKFVSLMLERC

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=36.9109947643979, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=37.597911227154, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=37.597911227154, Blast_Score=232, Evalue=6e-61,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=45.4545454545455, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI193204318, Length=380, Percent_Identity=37.6315789473684, Blast_Score=242, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=37.3096446700508, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=37.467018469657, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=35.8974358974359, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24642586, Length=400, Percent_Identity=35, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41944; Mature: 41944

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPYKKNAKLVLSNGIVFPGFFFGASGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFT
CCCCCCCCCEEEEECCEECCCCEECCCCCEEEEEEEECCCCHHHHHHCCCHHHCEEEEEE
YPEIGNTGINFEDSESNINIKGIIVRNFSSNNSNWRSKKNFNQWLVEKNIIGLYGIDTRA
CCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHH
LVKILRSTGAMNGVITSLDKTDESCLKIIQDTPKMEGLNLSKVVSTKQQYLWNSHTQTVF
HHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
DLRKRYDESSKKLKIVAIDFGIKNSILNRLVSHGCEVLVLPSRSSLNDVLSNKPDGIFFS
HHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEEEEC
NGPGDPSTVSEGIDLAKSLIEYGEIPMFGICLGHQIFGLALGGSTYKLSFGHRGLNHPCG
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHEEEEEECCCEEEEECCCCCCCCCCC
QNNKIEITSQNHGFAIDPNSLPKNLVRITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPG
CCCEEEEEECCCCEEECCCCCCCCEEEEEEEECCCCEEEEEEECCCEEEEEEECCCCCCC
PHDSDYLFKKFVSLMLERC
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINPYKKNAKLVLSNGIVFPGFFFGASGTAVGEIVFNTGMTGYQEVITDPSYYGQILTFT
CCCCCCCCCEEEEECCEECCCCEECCCCCEEEEEEEECCCCHHHHHHCCCHHHCEEEEEE
YPEIGNTGINFEDSESNINIKGIIVRNFSSNNSNWRSKKNFNQWLVEKNIIGLYGIDTRA
CCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHH
LVKILRSTGAMNGVITSLDKTDESCLKIIQDTPKMEGLNLSKVVSTKQQYLWNSHTQTVF
HHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
DLRKRYDESSKKLKIVAIDFGIKNSILNRLVSHGCEVLVLPSRSSLNDVLSNKPDGIFFS
HHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEEEEC
NGPGDPSTVSEGIDLAKSLIEYGEIPMFGICLGHQIFGLALGGSTYKLSFGHRGLNHPCG
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHEEEEEECCCEEEEECCCCCCCCCCC
QNNKIEITSQNHGFAIDPNSLPKNLVRITHYNLNDNTVAGLEVNNKPIFSVQYHPEAGPG
CCCEEEEEECCCCEEECCCCCCCCEEEEEEEECCCCEEEEEEECCCEEEEEEECCCCCCC
PHDSDYLFKKFVSLMLERC
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA