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The map label for this gene is leuB [H]

Identifier: 123968383

GI number: 123968383

Start: 736432

End: 737505

Strand: Reverse

Name: leuB [H]

Synonym: A9601_08501

Alternate gene names: 123968383

Gene position: 737505-736432 (Counterclockwise)

Preceding gene: 123968384

Following gene: 123968382

Centisome position: 44.16

GC content: 31.01

Gene sequence:

>1074_bases
ATGAAAAATTATAAGATTGTTTTATTGTCAGGAGACGGAATTGGACCAGAGATTTCAGAAGTTTCAAAAAAAGTTTTAAA
AAAGCTTTCAAAAAATCATAATTTCGATATTGAGATTATTGAAAAATTATTTGGAGGGATAGCATATGAAAAATATGGTA
CTCCTGCTCCAGATGAGACACTAGATCAATGCAAAAAAAGTGATGCAGTACTTTTAGCATGTGTTGGAGATATAAAATAT
GATTCTCTTGCAAGAGAATTAAGGCCAGAAAGTGGGTTACTAAAGTTAAGATCTGCTCTAAACCTTTTTGCAAATATCAG
GCCTGTCAAAATAAGAAAATCTCTCTTGGAAGCAAGTACATTAAAAAAAGAAATCGTTGAGAATGTAGATCTTATTGTTG
TAAGAGAATTAATAGGGGGAATTTATTTTGGAAAACCAAGAGGACATATAACAAATACAAAAATCCCAAAAGCTTTCAAT
ACAATGGTTTATGATTTGACCGAAATAGAAAGAATAACTGAAATAGCAATAAAAATTGCTAACCAAAGAAATAAAAAAAT
ATGTTCTGTTGATAAATCAAACGTTCTTGAGGTTAGTCAATTATGGAGAGATACAGTTTTAAATATCACCTCAAAAGATA
AAAATATATCCCTAAGCAATATGTACGTTGATAATGCAGCAATGCAATTAGTTAGAGATCCTGGTCAATTTGATGTGATT
TTAACTAGTAATTTATTTGGAGATATTTTAAGCGATTTAGCAGCAATGTTAACTGGTTCTATTGGTATGCTTCCATCTGC
TTCTTTAAACAATAATGGCCCAGGCGTTTTTGAACCTGTTCATGGTTCGGCTCCTGATATAGCTGGTAAAAACATAGCTA
ATCCTATTGCAATGCTTTTATCTGCTTCCATGATGTTAAAAATTGGATTAAATGAAGAAGAAGCTGCAGAAAATTTAGAA
ACTGCCATTGATAAAGTTTTATCAAAAGGATTTAGAACAGCAGATTTAGCTGATGGGTCTACTGAAGTTTTATCTTGCAG
TGAAATCGGAGATAAAATAATGTATGAAATTTAA

Upstream 100 bases:

>100_bases
CTAAGGAGTTCAAGTATTTTTAAAAAATTACCAGAATTAGCAAAAAAACTTAGACAATTAGACAAGCAATAATTTATTGT
TCTATTAAATAAAAATTTAA

Downstream 100 bases:

>100_bases
AAAAAAATTAATAAATAAAAAAATATTTTTAAGTAAAACATAAAGTTGGCATATGACCTGTCAGAATCATTAGATATTGT
TTTTAAAAAATGTCAAAACG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDETLDQCKKSDAVLLACVGDIKY
DSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLEASTLKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDLTEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAENLE
TAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIMYEI

Sequences:

>Translated_357_residues
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDETLDQCKKSDAVLLACVGDIKY
DSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLEASTLKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDLTEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAENLE
TAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIMYEI
>Mature_357_residues
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDETLDQCKKSDAVLLACVGDIKY
DSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLEASTLKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDLTEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAENLE
TAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIMYEI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=32.122905027933, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=30.0319488817891, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=30.0319488817891, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4758582, Length=218, Percent_Identity=33.9449541284404, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI28178838, Length=218, Percent_Identity=33.9449541284404, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=38.0281690140845, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=48.8700564971751, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=32.8571428571429, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1787381, Length=411, Percent_Identity=26.0340632603406, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=30.791788856305, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=27.9329608938547, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=345, Percent_Identity=28.1159420289855, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17505779, Length=351, Percent_Identity=28.2051282051282, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=44.8087431693989, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=36.7688022284123, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=31.700288184438, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=360, Percent_Identity=29.7222222222222, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=31.9209039548023, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=31.9209039548023, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24661184, Length=368, Percent_Identity=31.7934782608696, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=27.0114942528736, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=27.0114942528736, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=27.0114942528736, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=27.0114942528736, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=30.4597701149425, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=30.4597701149425, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078639, Length=344, Percent_Identity=27.3255813953488, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20130355, Length=228, Percent_Identity=25.8771929824561, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39059; Mature: 39059

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDET
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEHHCCCCCCHHH
LDQCKKSDAVLLACVGDIKYDSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLEAST
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFNTMVYDLTEIERITEIAIKIA
HHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
CCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHEEHHHHHHHHHCCCCCEEEE
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SASMMLKIGLNEEEAAENLETAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIMYEI
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEHHHHCCHHHHCC
>Mature Secondary Structure
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSKNHNFDIEIIEKLFGGIAYEKYGTPAPDET
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEHHCCCCCCHHH
LDQCKKSDAVLLACVGDIKYDSLARELRPESGLLKLRSALNLFANIRPVKIRKSLLEAST
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFNTMVYDLTEIERITEIAIKIA
HHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NQRNKKICSVDKSNVLEVSQLWRDTVLNITSKDKNISLSNMYVDNAAMQLVRDPGQFDVI
CCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHEEHHHHHHHHHCCCCCEEEE
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SASMMLKIGLNEEEAAENLETAIDKVLSKGFRTADLADGSTEVLSCSEIGDKIMYEI
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA