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The map label for this gene is menD

Identifier: 123968198

GI number: 123968198

Start: 585811

End: 587574

Strand: Direct

Name: menD

Synonym: A9601_06631

Alternate gene names: 123968198

Gene position: 585811-587574 (Clockwise)

Preceding gene: 123968195

Following gene: 123968199

Centisome position: 35.08

GC content: 31.12

Gene sequence:

>1764_bases
ATGACATCATCTATTGAATGCAAAAATTTTCTTAGAAGTTTACAACTTTTAAATCTTCTTATAAAAATAGGAGTTAAAAA
TTTAATACTATGTCCAGGAAGTAGATCAGCACCTCTAGCAATAGCGGCTGGAGAATTAAATAAGCTAGGACTGGTAAATA
TTTTTAATTCAATAGATGAGAGATCTGCTGGATTTCACTCTCTTGGGATTTCTGCTGCATCAGGTCATCTTTCTTTAGTT
ATTACCACTTCTGGAACTGCGGTAAGTAACTTATTGCCAGCAGCAGTTGAGGCAGACAGATCTTGTAAAGGTATTATATT
TCTTACCGCTGATAGACCCTTAAGATTAAAAGATTGTGGCGCTAATCAAACAGTAAATCAAGAAGATTTTTTGAGTTCAG
TCTGCAGAAAAGTTTTAAGTACAAATCTAAATGGAATCCATGAAACAGAAGAAAATGAAATCTTTAATTTAGTGCGAATT
TCTGAGAAACAAATGTCAACCTTCCCTGGACCTATACATTTAAATGTTCCTATTGATAAGCCTTTAGATATTTCATTCTT
AAATAAAAAAAATGTTTTAGAGGTTTTTGAGAGAATTTATTTAAAGAAAAAATATGTCTTTCAGGAAGTTGACATAAAGT
CTGATAAAAAAAAATTCTTAGAAATTTCAAAAAATTTAAATTTAGATGAGTCTGGCATTATTTTGGTAGGTCCCTATCAA
GGTTCCATAAATGACTTGCCTTCTTTTAATAAGTCTTTAGGACAATTACAAGAAATTACTGGTTGGCCAGTATTTGCTGA
TCCTGTTTCAGGCGTTTATTCTGATTTGAGAGGGTTAATTGTAAATTGGGAATTAGTCTTAAGGAAAAATAAGAATTTAA
TAAATTGTTATCAACTTTTGAGGCTTGGTCCTATGTCATCCTCAAATGATTTGGAAAAGTTTTTAATTAACTTCCAAGGG
GTACAAATTCTTATAAAGGAAAAAAACCATAGAAAACTGGACCCTATAAAAAAATCATTTGAATACGATTTTGGGTTAAC
AAATTTTACTTCTCTATTAAAAAAAGAGTTATCAATTAATGAAAAAAATAAAAAGTCTCTTACTCCGTTAGCCCTAGATT
TACTAGAGGAAGGTAAGCAAGTTAAAGAAATTTTAAAGGGGAATATTACTTATGAGAATCAAATTACTGAGTATAGGCTT
GCAAATCTTGTACCAAAACTTTGGCCAGCTGAAAATCCAATAATGCTTTCAGCGAGTAGCCCGATAAGAGATTGGCTTAC
ATTTTCTGAGAATGGTACTTTAACAAGAAATTGTTTCAGCTTTAGAGGAGCCTCTGGCATAGACGGTACTTTATCCCTTG
CATTAGGAATTTCTAGAATTAAAAATCCTCTACTTCTCGTTACAGGAGATTTGGCTTTTCTGCATGATATAAACGGTTGG
CTTATTGAAAACTCAATCGACATGAATTTAACAATTCTCCTAATAAACAATAATGGCGGAAACATATTTAATCGTATTTA
TAAAAAAAATTTAAAAGAAGATGAATTTAAAAAACTATTTCTTATGCCTAAAGAAATAAACTGGCCGAAACTTGCCGAGG
GTTACCAAGTAAACTTTAGAAGTGTGGCAAATTTTAAAAAATTACGAGAGGCATTCGATTGGAGCATTTCTATCGAGAAA
TCTGCAATAATTAAAGTTGATATTGATCCACAAAATGAAATTTGTGAAAAAAATTCTCTGCTAGAAAAAATAATTGGCAG
TTAA

Upstream 100 bases:

>100_bases
TTTTTTAAATCCTTTAGTATTGAATTTCTTTTTTCTTGAGTTGTAGACATGTTTTATTTAATTCAGTTATAAAGAAAATC
CCAAAGCATTTATATAAATT

Downstream 100 bases:

>100_bases
ATTTATGATTTTTTATCTTTGAATAATTAGAATTCATGAAAGTATTACCTGGTAAAACAAATTTAAATTGGTCAGAATGC
AAATCTTATGAGGATATATT

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTSSIECKNFLRSLQLLNLLIKIGVKNLILCPGSRSAPLAIAAGELNKLGLVNIFNSIDERSAGFHSLGISAASGHLSLV
ITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCGANQTVNQEDFLSSVCRKVLSTNLNGIHETEENEIFNLVRI
SEKQMSTFPGPIHLNVPIDKPLDISFLNKKNVLEVFERIYLKKKYVFQEVDIKSDKKKFLEISKNLNLDESGIILVGPYQ
GSINDLPSFNKSLGQLQEITGWPVFADPVSGVYSDLRGLIVNWELVLRKNKNLINCYQLLRLGPMSSSNDLEKFLINFQG
VQILIKEKNHRKLDPIKKSFEYDFGLTNFTSLLKKELSINEKNKKSLTPLALDLLEEGKQVKEILKGNITYENQITEYRL
ANLVPKLWPAENPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFLHDINGW
LIENSIDMNLTILLINNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWPKLAEGYQVNFRSVANFKKLREAFDWSISIEK
SAIIKVDIDPQNEICEKNSLLEKIIGS

Sequences:

>Translated_587_residues
MTSSIECKNFLRSLQLLNLLIKIGVKNLILCPGSRSAPLAIAAGELNKLGLVNIFNSIDERSAGFHSLGISAASGHLSLV
ITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCGANQTVNQEDFLSSVCRKVLSTNLNGIHETEENEIFNLVRI
SEKQMSTFPGPIHLNVPIDKPLDISFLNKKNVLEVFERIYLKKKYVFQEVDIKSDKKKFLEISKNLNLDESGIILVGPYQ
GSINDLPSFNKSLGQLQEITGWPVFADPVSGVYSDLRGLIVNWELVLRKNKNLINCYQLLRLGPMSSSNDLEKFLINFQG
VQILIKEKNHRKLDPIKKSFEYDFGLTNFTSLLKKELSINEKNKKSLTPLALDLLEEGKQVKEILKGNITYENQITEYRL
ANLVPKLWPAENPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFLHDINGW
LIENSIDMNLTILLINNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWPKLAEGYQVNFRSVANFKKLREAFDWSISIEK
SAIIKVDIDPQNEICEKNSLLEKIIGS
>Mature_586_residues
TSSIECKNFLRSLQLLNLLIKIGVKNLILCPGSRSAPLAIAAGELNKLGLVNIFNSIDERSAGFHSLGISAASGHLSLVI
TTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCGANQTVNQEDFLSSVCRKVLSTNLNGIHETEENEIFNLVRIS
EKQMSTFPGPIHLNVPIDKPLDISFLNKKNVLEVFERIYLKKKYVFQEVDIKSDKKKFLEISKNLNLDESGIILVGPYQG
SINDLPSFNKSLGQLQEITGWPVFADPVSGVYSDLRGLIVNWELVLRKNKNLINCYQLLRLGPMSSSNDLEKFLINFQGV
QILIKEKNHRKLDPIKKSFEYDFGLTNFTSLLKKELSINEKNKKSLTPLALDLLEEGKQVKEILKGNITYENQITEYRLA
NLVPKLWPAENPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFLHDINGWL
IENSIDMNLTILLINNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWPKLAEGYQVNFRSVANFKKLREAFDWSISIEKS
AIIKVDIDPQNEICEKNSLLEKIIGS

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=590, Percent_Identity=24.9152542372881, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PROMS (A2BQ88)

Other databases:

- EMBL:   CP000551
- RefSeq:   YP_001009056.1
- ProteinModelPortal:   A2BQ88
- STRING:   A2BQ88
- GeneID:   4717365
- GenomeReviews:   CP000551_GR
- KEGG:   pmb:A9601_06631
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   A2BQ88
- ProtClustDB:   CLSK921906
- BioCyc:   PMAR146891:A9601_06631-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 65873; Mature: 65742

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSIECKNFLRSLQLLNLLIKIGVKNLILCPGSRSAPLAIAAGELNKLGLVNIFNSIDE
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
RSAGFHSLGISAASGHLSLVITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCG
HCCCCHHCCCEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCC
ANQTVNQEDFLSSVCRKVLSTNLNGIHETEENEIFNLVRISEKQMSTFPGPIHLNVPIDK
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEECHHHHHCCCCCEEEECCCCC
PLDISFLNKKNVLEVFERIYLKKKYVFQEVDIKSDKKKFLEISKNLNLDESGIILVGPYQ
CCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCC
GSINDLPSFNKSLGQLQEITGWPVFADPVSGVYSDLRGLIVNWELVLRKNKNLINCYQLL
CCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHH
RLGPMSSSNDLEKFLINFQGVQILIKEKNHRKLDPIKKSFEYDFGLTNFTSLLKKELSIN
HCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
EKNKKSLTPLALDLLEEGKQVKEILKGNITYENQITEYRLANLVPKLWPAENPIMLSASS
CCCCCCCCHHHHHHHHCCHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEECCC
PIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFLHDINGW
CHHHHEEECCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEECCCCE
LIENSIDMNLTILLINNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWPKLAEGYQVNFR
EEECCCCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCCCEECHH
SVANFKKLREAFDWSISIEKSAIIKVDIDPQNEICEKNSLLEKIIGS
HHHHHHHHHHHHCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSIECKNFLRSLQLLNLLIKIGVKNLILCPGSRSAPLAIAAGELNKLGLVNIFNSIDE
CCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
RSAGFHSLGISAASGHLSLVITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCG
HCCCCHHCCCEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCC
ANQTVNQEDFLSSVCRKVLSTNLNGIHETEENEIFNLVRISEKQMSTFPGPIHLNVPIDK
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEECHHHHHCCCCCEEEECCCCC
PLDISFLNKKNVLEVFERIYLKKKYVFQEVDIKSDKKKFLEISKNLNLDESGIILVGPYQ
CCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCC
GSINDLPSFNKSLGQLQEITGWPVFADPVSGVYSDLRGLIVNWELVLRKNKNLINCYQLL
CCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHH
RLGPMSSSNDLEKFLINFQGVQILIKEKNHRKLDPIKKSFEYDFGLTNFTSLLKKELSIN
HCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
EKNKKSLTPLALDLLEEGKQVKEILKGNITYENQITEYRLANLVPKLWPAENPIMLSASS
CCCCCCCCHHHHHHHHCCHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEECCC
PIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFLHDINGW
CHHHHEEECCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEECCCCE
LIENSIDMNLTILLINNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWPKLAEGYQVNFR
EEECCCCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCCCEECHH
SVANFKKLREAFDWSISIEKSAIIKVDIDPQNEICEKNSLLEKIIGS
HHHHHHHHHHHHCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA