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The map label for this gene is priA [H]

Identifier: 123968089

GI number: 123968089

Start: 481797

End: 484073

Strand: Direct

Name: priA [H]

Synonym: A9601_05541

Alternate gene names: 123968089

Gene position: 481797-484073 (Clockwise)

Preceding gene: 123968086

Following gene: 123968093

Centisome position: 28.85

GC content: 31.09

Gene sequence:

>2277_bases
TTGAAGCCGGCAAGTAATCAGTTATTAAATATCTCCTATAAATTGGAAATTTTGCTTGATATAGGTAGTGGTAATGAAAG
CTTTTACTATTTAGATGGAAATAATCTTGGAGCAGAAGTTGGAGATATTGTAAGTGTCAGACTAAGGGGGAGATTATTGA
ATGGGTTGGTAATCTCCAAAAAAGACTTTTCGACAATCAATAATGATGAATCAAATATTACTGGAGGAAAAAGCATAAGA
TATTTGTTTGTTGAAAGTATTTTGCAGAAAAAAATAATTGATGAATCTTGGAGAGAATTGATAGACTTCCTAGCCTCTTT
TTATATGGTTAGTAATTTAAAAATGTTTAAAACTGCATTTCCTCCTGGCTGGATTGGTAAATATAAAAATTTCTCTAAAG
GATTAAAAGATCAAATATGGATTGAAACTAAAAAAGAATTTGATATTAAGAAAAATGGATTAACCAAAAAAGAATTTTTT
TTAATGGATACTTTATCTAAAAAAGGTAATTGGCAAAGTGAATTAATAAAGTCTGGTTTTAATTACACACTAATTAACTC
AATGGTCAGTAAAAATTATCTTGTTAAATCTAGAAGAAAAAAAAATATAAATAGTAAATTAAATTCCTTTTTAAAAGATC
ATATCGCAACGAAAAAACCAAATCTTACAAATGAGCAAAAAATTGCATTTCAAGAATTTCTAACAATGAAACCAGGAGAT
GCATTACTTCTTTGGGGCGAAACAGGTTCAGGTAAAACAGAAGTGTATATGAGAATTGCTGAAGATCAATTTCTTAAGAA
AAAAAGTTGTTTGATACTAGCCCCAGAAATTGGATTAATTCCTCAACTTATTGATAGGTTTAGTAGGCGATTTAATAATG
TTGTTTACGAATATCATAGTAATTGTTCTCCCGAGCATAGAACTTTAATTTGGAAGAAAATTATTAAATCTAATGAACCT
TTAATAGTAATAGGTACAAGGTCGGCAGTTTTTCTTCCAATCAAAAATCTGGGAGTAATAATAATGGATGAAGAACATGA
TGTTTCTTATAAGCAAGATAGTCCTATGCCTTGCTATGACGCAAGAGAGATTGCTATTGAAATAGTAAAAAGGAATTCTG
CAAAGTTAATTTTTGGGAGTGCAACCCCATCAATGAAGACTTGGAAAAAGTGTATTTTTGAAAGGAATTTTAAATTGGTA
AGAATGATTCAAAGGATATCCAGTAATGAGACTCCTGAAATAAAAATTATTGATATGCGGGATGAGTTCAAAATGGGAAA
TATGAAAATTTTTTCCAATGAATTATTACAATTGCTTCCTCAACTACGCTTAAAAAAAGAGCAAGCAATAATTTTAATCC
CTAGGAGGGGGCATAGTGGATTTTTAAGTTGTAGAAATTGCGGATATTTAATAAATTGCCCCAACTGTGACGTTCCTTTA
TCAGTTCATCTAGGATCACAAGGAAAAAAATGGTTAAGGTGTCATTGGTGTGATCATAAATCAAGATTGATCAATCGTTG
CCCAGATTGTCATTCGACTGCTTTCAAACCTTTTGGAATTGGTACGCAAAGGGTAATAGAATTTTTAAATGAAGAATTTC
CTGACTTAAGAGTACTTCGCTTTGATAGAGATACAACTTCAGGAAAGGATGGTCATAGAGATATTCTTTCAAAGTTTTCT
AAAGGTGATGCTGATATTCTTGTCGGTACTCAGATGTTGGCAAAAGGTATTGACATCCCCAATATTACTCTTTCAGTTGT
TATTGCAGCAGATGGGTTGCTTCATCGCCCAGATATTTCAACAGAAGAAAAATCATTACAATTATTTTTGCAAGTAGCAG
GAAGGGCCGGCAGGGCACAAAAAAAAGGGAAAGTAATTTTTCAAACATATAAACCTAACCACCCGGTGATTTCGTATCTT
CAAAAAAGAGATTATGAAAGATTCTTAATTGAAAACTCTAGATTGAGAAAGGATGCTAATTTATTTCCATTTTGCACAAT
TTGCCTACTTAAATTATCAGGAGAAAATTATGAATTAACTGAGTCAATTGCAATAAAATTAGCAAAATATCTACTCAGTT
TTTGTGAGAAAAAGAACTGGAAATTAATTGGTCCTGCCCCTAGTTTAATAGCTAAAGTCGGAAAAAAATTTAGATGGCAG
ATATTAATACATGGTCCTGAAGGAACAAAGATACCTTTACCTGATAGATCAATATTATGGAAACTAATTCCAAAAAATGT
TTTTTTAACAATAGATGTTAATCCAGCAGAGTTGTAA

Upstream 100 bases:

>100_bases
TCAATGATCAGAGCTTCATGTCTCCCTTAAGAGCAGGATACTTACAATGTGCAAAAAACACTTTGTGGAATGTTCAACAA
TCCAATACTAAGAAAAAGTT

Downstream 100 bases:

>100_bases
TTAGTTTGATGGAAGTTGAGGTAAATCTGAACCTAATTTAAATCTATAGAATCTTAATAAATAAGCCAATATTATAATTA
TTAAAATAATAGATATCCCA

Product: primosomal protein N' (replication factor Y)

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MKPASNQLLNISYKLEILLDIGSGNESFYYLDGNNLGAEVGDIVSVRLRGRLLNGLVISKKDFSTINNDESNITGGKSIR
YLFVESILQKKIIDESWRELIDFLASFYMVSNLKMFKTAFPPGWIGKYKNFSKGLKDQIWIETKKEFDIKKNGLTKKEFF
LMDTLSKKGNWQSELIKSGFNYTLINSMVSKNYLVKSRRKKNINSKLNSFLKDHIATKKPNLTNEQKIAFQEFLTMKPGD
ALLLWGETGSGKTEVYMRIAEDQFLKKKSCLILAPEIGLIPQLIDRFSRRFNNVVYEYHSNCSPEHRTLIWKKIIKSNEP
LIVIGTRSAVFLPIKNLGVIIMDEEHDVSYKQDSPMPCYDAREIAIEIVKRNSAKLIFGSATPSMKTWKKCIFERNFKLV
RMIQRISSNETPEIKIIDMRDEFKMGNMKIFSNELLQLLPQLRLKKEQAIILIPRRGHSGFLSCRNCGYLINCPNCDVPL
SVHLGSQGKKWLRCHWCDHKSRLINRCPDCHSTAFKPFGIGTQRVIEFLNEEFPDLRVLRFDRDTTSGKDGHRDILSKFS
KGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDISTEEKSLQLFLQVAGRAGRAQKKGKVIFQTYKPNHPVISYL
QKRDYERFLIENSRLRKDANLFPFCTICLLKLSGENYELTESIAIKLAKYLLSFCEKKNWKLIGPAPSLIAKVGKKFRWQ
ILIHGPEGTKIPLPDRSILWKLIPKNVFLTIDVNPAEL

Sequences:

>Translated_758_residues
MKPASNQLLNISYKLEILLDIGSGNESFYYLDGNNLGAEVGDIVSVRLRGRLLNGLVISKKDFSTINNDESNITGGKSIR
YLFVESILQKKIIDESWRELIDFLASFYMVSNLKMFKTAFPPGWIGKYKNFSKGLKDQIWIETKKEFDIKKNGLTKKEFF
LMDTLSKKGNWQSELIKSGFNYTLINSMVSKNYLVKSRRKKNINSKLNSFLKDHIATKKPNLTNEQKIAFQEFLTMKPGD
ALLLWGETGSGKTEVYMRIAEDQFLKKKSCLILAPEIGLIPQLIDRFSRRFNNVVYEYHSNCSPEHRTLIWKKIIKSNEP
LIVIGTRSAVFLPIKNLGVIIMDEEHDVSYKQDSPMPCYDAREIAIEIVKRNSAKLIFGSATPSMKTWKKCIFERNFKLV
RMIQRISSNETPEIKIIDMRDEFKMGNMKIFSNELLQLLPQLRLKKEQAIILIPRRGHSGFLSCRNCGYLINCPNCDVPL
SVHLGSQGKKWLRCHWCDHKSRLINRCPDCHSTAFKPFGIGTQRVIEFLNEEFPDLRVLRFDRDTTSGKDGHRDILSKFS
KGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDISTEEKSLQLFLQVAGRAGRAQKKGKVIFQTYKPNHPVISYL
QKRDYERFLIENSRLRKDANLFPFCTICLLKLSGENYELTESIAIKLAKYLLSFCEKKNWKLIGPAPSLIAKVGKKFRWQ
ILIHGPEGTKIPLPDRSILWKLIPKNVFLTIDVNPAEL
>Mature_758_residues
MKPASNQLLNISYKLEILLDIGSGNESFYYLDGNNLGAEVGDIVSVRLRGRLLNGLVISKKDFSTINNDESNITGGKSIR
YLFVESILQKKIIDESWRELIDFLASFYMVSNLKMFKTAFPPGWIGKYKNFSKGLKDQIWIETKKEFDIKKNGLTKKEFF
LMDTLSKKGNWQSELIKSGFNYTLINSMVSKNYLVKSRRKKNINSKLNSFLKDHIATKKPNLTNEQKIAFQEFLTMKPGD
ALLLWGETGSGKTEVYMRIAEDQFLKKKSCLILAPEIGLIPQLIDRFSRRFNNVVYEYHSNCSPEHRTLIWKKIIKSNEP
LIVIGTRSAVFLPIKNLGVIIMDEEHDVSYKQDSPMPCYDAREIAIEIVKRNSAKLIFGSATPSMKTWKKCIFERNFKLV
RMIQRISSNETPEIKIIDMRDEFKMGNMKIFSNELLQLLPQLRLKKEQAIILIPRRGHSGFLSCRNCGYLINCPNCDVPL
SVHLGSQGKKWLRCHWCDHKSRLINRCPDCHSTAFKPFGIGTQRVIEFLNEEFPDLRVLRFDRDTTSGKDGHRDILSKFS
KGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDISTEEKSLQLFLQVAGRAGRAQKKGKVIFQTYKPNHPVISYL
QKRDYERFLIENSRLRKDANLFPFCTICLLKLSGENYELTESIAIKLAKYLLSFCEKKNWKLIGPAPSLIAKVGKKFRWQ
ILIHGPEGTKIPLPDRSILWKLIPKNVFLTIDVNPAEL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=629, Percent_Identity=33.3863275039746, Blast_Score=340, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86910; Mature: 86910

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS00675 SIGMA54_INTERACT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASNQLLNISYKLEILLDIGSGNESFYYLDGNNLGAEVGDIVSVRLRGRLLNGLVISK
CCCCCCCEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHEECCCEEEEC
KDFSTINNDESNITGGKSIRYLFVESILQKKIIDESWRELIDFLASFYMVSNLKMFKTAF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
PPGWIGKYKNFSKGLKDQIWIETKKEFDIKKNGLTKKEFFLMDTLSKKGNWQSELIKSGF
CCCHHHCCCHHHCCCCCEEEEEECCCCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHCCC
NYTLINSMVSKNYLVKSRRKKNINSKLNSFLKDHIATKKPNLTNEQKIAFQEFLTMKPGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
ALLLWGETGSGKTEVYMRIAEDQFLKKKSCLILAPEIGLIPQLIDRFSRRFNNVVYEYHS
EEEEEECCCCCCEEEEEEEHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
NCSPEHRTLIWKKIIKSNEPLIVIGTRSAVFLPIKNLGVIIMDEEHDVSYKQDSPMPCYD
CCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCC
AREIAIEIVKRNSAKLIFGSATPSMKTWKKCIFERNFKLVRMIQRISSNETPEIKIIDMR
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECC
DEFKMGNMKIFSNELLQLLPQLRLKKEQAIILIPRRGHSGFLSCRNCGYLINCPNCDVPL
CCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCEEEECCCCCCEE
SVHLGSQGKKWLRCHWCDHKSRLINRCPDCHSTAFKPFGIGTQRVIEFLNEEFPDLRVLR
EEEECCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
FDRDTTSGKDGHRDILSKFSKGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDIS
ECCCCCCCCCCHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCEEEEEEECCCCEECCCCC
TEEKSLQLFLQVAGRAGRAQKKGKVIFQTYKPNHPVISYLQKRDYERFLIENSRLRKDAN
CCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHHHHHCCCHHCCCCC
LFPFCTICLLKLSGENYELTESIAIKLAKYLLSFCEKKNWKLIGPAPSLIAKVGKKFRWQ
CCHHHHHHHHEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEE
ILIHGPEGTKIPLPDRSILWKLIPKNVFLTIDVNPAEL
EEEECCCCCCCCCCCHHHHHEECCCCEEEEEECCCCCC
>Mature Secondary Structure
MKPASNQLLNISYKLEILLDIGSGNESFYYLDGNNLGAEVGDIVSVRLRGRLLNGLVISK
CCCCCCCEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHEECCCEEEEC
KDFSTINNDESNITGGKSIRYLFVESILQKKIIDESWRELIDFLASFYMVSNLKMFKTAF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
PPGWIGKYKNFSKGLKDQIWIETKKEFDIKKNGLTKKEFFLMDTLSKKGNWQSELIKSGF
CCCHHHCCCHHHCCCCCEEEEEECCCCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHCCC
NYTLINSMVSKNYLVKSRRKKNINSKLNSFLKDHIATKKPNLTNEQKIAFQEFLTMKPGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
ALLLWGETGSGKTEVYMRIAEDQFLKKKSCLILAPEIGLIPQLIDRFSRRFNNVVYEYHS
EEEEEECCCCCCEEEEEEEHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
NCSPEHRTLIWKKIIKSNEPLIVIGTRSAVFLPIKNLGVIIMDEEHDVSYKQDSPMPCYD
CCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCC
AREIAIEIVKRNSAKLIFGSATPSMKTWKKCIFERNFKLVRMIQRISSNETPEIKIIDMR
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECC
DEFKMGNMKIFSNELLQLLPQLRLKKEQAIILIPRRGHSGFLSCRNCGYLINCPNCDVPL
CCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCEEEECCCCCCEE
SVHLGSQGKKWLRCHWCDHKSRLINRCPDCHSTAFKPFGIGTQRVIEFLNEEFPDLRVLR
EEEECCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
FDRDTTSGKDGHRDILSKFSKGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDIS
ECCCCCCCCCCHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCEEEEEEECCCCEECCCCC
TEEKSLQLFLQVAGRAGRAQKKGKVIFQTYKPNHPVISYLQKRDYERFLIENSRLRKDAN
CCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHHHHHCCCHHCCCCC
LFPFCTICLLKLSGENYELTESIAIKLAKYLLSFCEKKNWKLIGPAPSLIAKVGKKFRWQ
CCHHHHHHHHEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEE
ILIHGPEGTKIPLPDRSILWKLIPKNVFLTIDVNPAEL
EEEECCCCCCCCCCCHHHHHEECCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]