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The map label for this gene is murE [H]

Identifier: 123968000

GI number: 123968000

Start: 402426

End: 403961

Strand: Direct

Name: murE [H]

Synonym: A9601_04631

Alternate gene names: 123968000

Gene position: 402426-403961 (Clockwise)

Preceding gene: 123967999

Following gene: 123968001

Centisome position: 24.1

GC content: 29.69

Gene sequence:

>1536_bases
ATGAGATCTATAAAATTACATAAACTTTTAGATTTGGTAGGAATTATTCCTTCATCAAATCTTATCGATCAAGAAATCAA
TAATATTTCTTTTAACTCTAAAGAAGTACAAAAAGGAACTTTATTTTTAGGAATGCCTGGTTTAAATGTTGATGGAGGAA
AATTTTGTATTGAGGCAATTGAAAATGGCGCAGAGGCCGCCATTATTGGCCCTGCTGCAAAACAGAAAATTGGATCTATT
GATCGAAAAAGGATTTTGGTTATAGAGGATAATTTAGATTATATTTTTGGTCAAATAGTCGCTGAGTTTTGGAATAGGCC
TTCAAGAAAACTTAAACTTATTGGTGTTACTGGTACAAATGGAAAAACGACGATTACTTTTTTATTGGAATATCTTTTAA
AAAAATTAGGAAAAAAAACTGCATTGTTTGGGACTTTATTCAATAGATGGCCCGGTTTCTCTGAAGTAGCTTCTCATACA
ACTGATTTCGCTGATAAACTTCAAAATAAATTAAATGCTGCTCTTGAGGCAGAATCTGAATTCGCGATATTAGAGGTAAG
TTCTCATTCTATTGCTCAAAACAGGATATCAGGATGCGAATTTGAGGCGGCTATTTTTACTAATTTAACTCAAGATCATC
TTGATTATCACCCAGATATGGAATCATATTTTCAAACAAAAAGAAAATTATTTTTCCCACCTTACTTAAAAGAAAAAGAT
GGGATTTGTGTATTAAATCATGATGACCATTGGATATCTAAATTATCATCTGATCTTAAAAAAAGATCTTTATTAGTCTC
TACAAAGATTACTGAAAGTGAATTTGAAAATGATGATTTTTTTTTCGTAACAGATAAAAAATTTACTGAAAGTGGTTCCA
CTTGTATTTTTCATACCCCTAGGGAAAAAATTCAACTTTTTGTTCCACTTGTTGGTGAATTTAATTTAATGAATACGATT
CAAGCAATAACAATTTTGTATAAACTGAATTTTTCTTTAAAAGATTTATCAAAGATAATACAATCTTTCCCTGGTGCTCC
TGGGAGAATGGAGAAAATACAAATTGATGATAATGACGTTTCAAGATCTCTTCCAACAGTAATTGTTGATTATGCCCACA
CTCCTGATGGATTAAAAAAAGTTTTGCAATCAATTAAAAAACTTTGTGAAGGGAAACTAATAACTGTTTTTGGCTGTGGC
GGAGATCGTGATCGTAGTAAAAGGCCTTTGATGGGATCAATAGCTGAAGAGTTGTCTGATCAACTTTTTATAACCTCAGA
TAATCCAAGAACAGAAGAACCCCAAAAGATAGTAAATGATATTTTGATGGGTATAAAAAAAAGAGAAAAAATAATAATTG
AAATTGATAGATTTAAAGCAATAAATGAATCTATTCAATTTGCCAATAAAAAAGATATTGTTTTAATTGCAGGCAAAGGA
CATGAAGACTACCAAATTCTCAATGATAAAGTTATTAATTTTGATGATAGAAAAATAGCTTATAAATTATTAAAAGAAAA
AAATAAATCTCAATAA

Upstream 100 bases:

>100_bases
TATAGAATTGCCTCGCATTTCTCCAAGATTAAAAGATGATCAATTAAAGAATTGGTTTCAAAAAAATATTAGTACCATTC
TGGAGAAATAATTTTTTTAT

Downstream 100 bases:

>100_bases
AATTTACTAATTAAATAAGAAAGATTTTTACGCAAATCACAAGAAAAGTTTCTTAGAATCAATGTAGTTATTTTTAATAA
AATGAAAGGCTTAGCCTTAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRSIKLHKLLDLVGIIPSSNLIDQEINNISFNSKEVQKGTLFLGMPGLNVDGGKFCIEAIENGAEAAIIGPAAKQKIGSI
DRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALFGTLFNRWPGFSEVASHT
TDFADKLQNKLNAALEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHPDMESYFQTKRKLFFPPYLKEKD
GICVLNHDDHWISKLSSDLKKRSLLVSTKITESEFENDDFFFVTDKKFTESGSTCIFHTPREKIQLFVPLVGEFNLMNTI
QAITILYKLNFSLKDLSKIIQSFPGAPGRMEKIQIDDNDVSRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCG
GDRDRSKRPLMGSIAEELSDQLFITSDNPRTEEPQKIVNDILMGIKKREKIIIEIDRFKAINESIQFANKKDIVLIAGKG
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ

Sequences:

>Translated_511_residues
MRSIKLHKLLDLVGIIPSSNLIDQEINNISFNSKEVQKGTLFLGMPGLNVDGGKFCIEAIENGAEAAIIGPAAKQKIGSI
DRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALFGTLFNRWPGFSEVASHT
TDFADKLQNKLNAALEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHPDMESYFQTKRKLFFPPYLKEKD
GICVLNHDDHWISKLSSDLKKRSLLVSTKITESEFENDDFFFVTDKKFTESGSTCIFHTPREKIQLFVPLVGEFNLMNTI
QAITILYKLNFSLKDLSKIIQSFPGAPGRMEKIQIDDNDVSRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCG
GDRDRSKRPLMGSIAEELSDQLFITSDNPRTEEPQKIVNDILMGIKKREKIIIEIDRFKAINESIQFANKKDIVLIAGKG
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ
>Mature_511_residues
MRSIKLHKLLDLVGIIPSSNLIDQEINNISFNSKEVQKGTLFLGMPGLNVDGGKFCIEAIENGAEAAIIGPAAKQKIGSI
DRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALFGTLFNRWPGFSEVASHT
TDFADKLQNKLNAALEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHPDMESYFQTKRKLFFPPYLKEKD
GICVLNHDDHWISKLSSDLKKRSLLVSTKITESEFENDDFFFVTDKKFTESGSTCIFHTPREKIQLFVPLVGEFNLMNTI
QAITILYKLNFSLKDLSKIIQSFPGAPGRMEKIQIDDNDVSRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCG
GDRDRSKRPLMGSIAEELSDQLFITSDNPRTEEPQKIVNDILMGIKKREKIIIEIDRFKAINESIQFANKKDIVLIAGKG
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=488, Percent_Identity=36.4754098360656, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1786274, Length=368, Percent_Identity=22.554347826087, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 57791; Mature: 57791

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIKLHKLLDLVGIIPSSNLIDQEINNISFNSKEVQKGTLFLGMPGLNVDGGKFCIEAI
CCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
ENGAEAAIIGPAAKQKIGSIDRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTN
HCCCCEEEECCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GKTTITFLLEYLLKKLGKKTALFGTLFNRWPGFSEVASHTTDFADKLQNKLNAALEAESE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHPDMESYFQTKRKLFFPPYLKEKD
EEEEEECCCHHHHHHCCCCCCHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
GICVLNHDDHWISKLSSDLKKRSLLVSTKITESEFENDDFFFVTDKKFTESGSTCIFHTP
CEEEECCCHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCEEEEECCCCCCCCCEEEEECC
REKIQLFVPLVGEFNLMNTIQAITILYKLNFSLKDLSKIIQSFPGAPGRMEKIQIDDNDV
HHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHH
SRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCGGDRDRSKRPLMGSIAEELSD
HHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCC
QLFITSDNPRTEEPQKIVNDILMGIKKREKIIIEIDRFKAINESIQFANKKDIVLIAGKG
CEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEEEECCC
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ
CCCHHHHCCCEECCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSIKLHKLLDLVGIIPSSNLIDQEINNISFNSKEVQKGTLFLGMPGLNVDGGKFCIEAI
CCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
ENGAEAAIIGPAAKQKIGSIDRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTN
HCCCCEEEECCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GKTTITFLLEYLLKKLGKKTALFGTLFNRWPGFSEVASHTTDFADKLQNKLNAALEAESE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHPDMESYFQTKRKLFFPPYLKEKD
EEEEEECCCHHHHHHCCCCCCHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
GICVLNHDDHWISKLSSDLKKRSLLVSTKITESEFENDDFFFVTDKKFTESGSTCIFHTP
CEEEECCCHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCEEEEECCCCCCCCCEEEEECC
REKIQLFVPLVGEFNLMNTIQAITILYKLNFSLKDLSKIIQSFPGAPGRMEKIQIDDNDV
HHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHH
SRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCGGDRDRSKRPLMGSIAEELSD
HHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCC
QLFITSDNPRTEEPQKIVNDILMGIKKREKIIIEIDRFKAINESIQFANKKDIVLIAGKG
CEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCEEEEECCC
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ
CCCHHHHCCCEECCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]