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The map label for this gene is metK [H]

Identifier: 123967872

GI number: 123967872

Start: 307761

End: 309002

Strand: Reverse

Name: metK [H]

Synonym: A9601_03351

Alternate gene names: 123967872

Gene position: 309002-307761 (Counterclockwise)

Preceding gene: 123967873

Following gene: 123967871

Centisome position: 18.5

GC content: 37.12

Gene sequence:

>1242_bases
ATGAGTGATTTCATTTTCACTTCAGAATCAGTAACTGAAGGTCATCCTGACAAAATATGTGATCAAATTAGTGACGCTGT
TTTAGACGCTTTATTGACAGAAGATCCAGAAAGCAGAGTTGCATGCGAAACTGTTGTAAATACGGGTCTTTGTTTACTTA
CTGGAGAAATAACTTCAAAAGCAAAAGTCGATTATATCAAACTTGTTAGAAATGTAATTAAAGAAATTGGATATGAGGGC
TATAGAGCAGGTGGTTTTGACGCAAATAGTTGTGCAGTTTTAGTAGCACTTGACGAGCAATCACCAGATATTTCTCAAGG
AGTAAACGAAGCAGATGATGTTAACGATGATTTGGAAGATAATACCGGTGCTGGCGACCAAGGCATAATGTTCGGCTATG
CATGCGATGAGACGCCTGAATTAATGCCCTTACCAATTAGCTTGGCTCATAGATTAGCCATTCAACTTTCTAAGGTAAGG
CACGAAAACATGCTTAATTATCTACTCCCTGATGGTAAAACTCAAGTAAGTATTGATTATAAAAATGGAGTGCCACTATC
GATTAATACCATTTTAATTTCAACTCAACATAATCCTGAGATAGATGGTATTACAAATGAAGAAGAAATTCGTCAAAGAA
TAAAAGAAGATTTATGGAAGAATGTTGTACTCCCTGCTACTGAGGATTTAGAAATCAAACCAAACATTCAGACAACAAGA
TTTCTAGTCAATCCCACGGGCAAATTTGTCGTAGGGGGGCCTCAAGGTGATGCGGGACTTACTGGAAGAAAAATTATAGT
AGATACTTATGGGGGTTACGCAAGACACGGAGGAGGGGCATTTTCGGGTAAAGATCCCACAAAAGTGGATAGATCAGCTG
CTTATGCAGCACGTTACGTAGCAAAAAGTATAGTTAAGGCAAAATTAGCAAAAAAGGCAGAAGTACAATTAAGTTATGCA
ATTGGAGTTGCAAAACCGATTTCCATTCTTGTTGAAACTTTTGATACTGGCGTTATTTCACAAGCTAATTTGACTGAGCT
CATTAATAAGTACTTTGATTTAAGACCAGCAGCAATTATAAAAGAATTTGATCTAAGAAATCTACCCCAAAAAATGGGTG
GTACATTTTTCAGAAAGACTGCATCCTATGGACATTTTGGCAGAAGAGATTTAGATCTTCCTTGGGAAAAGGTAGAAGAA
AAAGCAGCCCAATTAGCAGAGGCTTCCAAAGTATTTTTATAA

Upstream 100 bases:

>100_bases
AAGCCTCATTAATTCTTATACAATTATTTTTAATTAACAATAATTGATTTGTAACGATAATCTAATTTAGGATATTTCAT
AATTCCAAAATTATTTGTAA

Downstream 100 bases:

>100_bases
AAATATTTATTCTATGCCTGATAATTTTTATGGAGGGTTAGATTTTGGAACCAGTGGTGCAAGAATATCAATAATTAATC
TTCATAAAAAATTAATATAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEG
YRAGGFDANSCAVLVALDEQSPDISQGVNEADDVNDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLSKVR
HENMLNYLLPDGKTQVSIDYKNGVPLSINTILISTQHNPEIDGITNEEEIRQRIKEDLWKNVVLPATEDLEIKPNIQTTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVETFDTGVISQANLTELINKYFDLRPAAIIKEFDLRNLPQKMGGTFFRKTASYGHFGRRDLDLPWEKVEE
KAAQLAEASKVFL

Sequences:

>Translated_413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEG
YRAGGFDANSCAVLVALDEQSPDISQGVNEADDVNDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLSKVR
HENMLNYLLPDGKTQVSIDYKNGVPLSINTILISTQHNPEIDGITNEEEIRQRIKEDLWKNVVLPATEDLEIKPNIQTTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVETFDTGVISQANLTELINKYFDLRPAAIIKEFDLRNLPQKMGGTFFRKTASYGHFGRRDLDLPWEKVEE
KAAQLAEASKVFL
>Mature_412_residues
SDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEGY
RAGGFDANSCAVLVALDEQSPDISQGVNEADDVNDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLSKVRH
ENMLNYLLPDGKTQVSIDYKNGVPLSINTILISTQHNPEIDGITNEEEIRQRIKEDLWKNVVLPATEDLEIKPNIQTTRF
LVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYAI
GVAKPISILVETFDTGVISQANLTELINKYFDLRPAAIIKEFDLRNLPQKMGGTFFRKTASYGHFGRRDLDLPWEKVEEK
AAQLAEASKVFL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=54.6599496221663, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=53.6523929471033, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1789311, Length=409, Percent_Identity=54.5232273838631, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=403, Percent_Identity=54.8387096774194, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=411, Percent_Identity=53.5279805352798, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538498, Length=398, Percent_Identity=54.0201005025126, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538494, Length=398, Percent_Identity=53.5175879396985, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565905, Length=398, Percent_Identity=46.4824120603015, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=357, Percent_Identity=52.1008403361345, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565911, Length=352, Percent_Identity=51.1363636363636, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=338, Percent_Identity=47.3372781065089, Blast_Score=281, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=70.3703703703704, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=76.9230769230769, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=398, Percent_Identity=52.5125628140703, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320710, Length=398, Percent_Identity=51.5075376884422, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=400, Percent_Identity=52.5, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580529, Length=400, Percent_Identity=52.5, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580537, Length=400, Percent_Identity=52.5, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580533, Length=400, Percent_Identity=52.5, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580535, Length=400, Percent_Identity=52.5, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580531, Length=400, Percent_Identity=52.5, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580527, Length=400, Percent_Identity=51.75, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580525, Length=400, Percent_Identity=51.75, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580541, Length=387, Percent_Identity=50.6459948320413, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=58.0645161290323, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45202; Mature: 45071

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSK
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AKVDYIKLVRNVIKEIGYEGYRAGGFDANSCAVLVALDEQSPDISQGVNEADDVNDDLED
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCCCHHHCCCCHHCCCCCCCC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLSKVRHENMLNYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KNGVPLSINTILISTQHNPEIDGITNEEEIRQRIKEDLWKNVVLPATEDLEIKPNIQTTR
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCCEECCCCCEEE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVETFDTGVISQANLTELINKYFDLRPAAII
HHHHHHHHHHHHHHEEEEEHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHH
KEFDLRNLPQKMGGTFFRKTASYGHFGRRDLDLPWEKVEEKAAQLAEASKVFL
HHHHHHCCHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSK
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AKVDYIKLVRNVIKEIGYEGYRAGGFDANSCAVLVALDEQSPDISQGVNEADDVNDDLED
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCCCHHHCCCCHHCCCCCCCC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLSKVRHENMLNYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KNGVPLSINTILISTQHNPEIDGITNEEEIRQRIKEDLWKNVVLPATEDLEIKPNIQTTR
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCCEECCCCCEEE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVETFDTGVISQANLTELINKYFDLRPAAII
HHHHHHHHHHHHHHEEEEEHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHH
KEFDLRNLPQKMGGTFFRKTASYGHFGRRDLDLPWEKVEEKAAQLAEASKVFL
HHHHHHCCHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA