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The map label for this gene is tgt [H]

Identifier: 123967830

GI number: 123967830

Start: 270815

End: 271933

Strand: Direct

Name: tgt [H]

Synonym: A9601_02931

Alternate gene names: 123967830

Gene position: 270815-271933 (Clockwise)

Preceding gene: 123967828

Following gene: 123967831

Centisome position: 16.22

GC content: 34.41

Gene sequence:

>1119_bases
GTGTTTGAATTTGAAATTACATCGAATTGCAGTAATACAGCAGCAAGAACTGGTATATTTCATACACCAAATGGTCAGGT
AAACACACCAAAATTTATGCCTGTGGGTACTTTGGCAACGGTTAAAGGAATTTCATCTAAGCAGTTAACCTCCACAGGAT
CAGAAATGATTCTTTCAAATACCTTTCATCTGCATTTACAACCTGGAGAAAAACTAGTTAAAGAATCTGGCGGAATACAT
AAGTTCATGAATTGGCCTAAGCCTATTCTTACTGATTCAGGAGGATATCAAGTTTTTAGTTTGGCCAAATTAAATAATAT
TTCTGATAAAGGCGTGGAATTTAAAAATCCAAGAGATGGTAGTTATGTATTTTTATCACCTGAAAAAGTAATACAGATTC
AAATGGATCTTGGATCGGATGTCGCGATGGCTTTTGATCATTGTCCTCCGCATACAGCTAACGAAAATGATATTGAGGAC
TCTTTACAAAGAACTCATTCATGGTTACAAAAAAGTGTTGAGACTCATCAGAAATCTAATCAAGCATTATTCGGTATAGT
TCAAGGTGGAAAGTATCCTAAATTGAGAGAATACAGTGCAAAATATACAAGTTCTTTTGATCTTCCTGGAATAGCAGTGG
GAGGTGTAAGTGTTGGCGAGACAGTCGAAGAAATACATAGTGTAATTAATTACGTCCCGAAATTCTTACCAATAAAAAAA
CCAAGATATTTAATGGGAATTGGCTCCTTAAAAGAAATTTCTCTTGCTGTTGCTAATGGATTCGATATATTTGACTGTGT
TTTGCCTACAAGACTAGGAAGACATGGGACTGCATTTTTTAATGATGAAAGACTGAATTTGCGAAATGCTCGATTTAAAA
ATGACTTTTCTCCGATTGATAAAACTTGTAAATGTGAAACCTGTAAATCCTATTCTCGAGCATATTTGCATCATCTAATT
AGAAATGACGAAATATTAGGACTAACTCTCATCAGTTTGCATAATATTGCTCACTTAATAAGATTTACCAATGCAATTTC
TACTGCAATTAGAGATAATTGTTTTACAAATGATTTCGCTCCTTGGAAAACATCCTCTATTGCTCACCATACGTGGTAA

Upstream 100 bases:

>100_bases
AAATTTTTTATTAAATATTTTTTGATGATATAAAAATAGATTCAATATTAAATAATAGGGTAGATTAATGAAAAAGGATC
AAAAAATTTTATAAATTATC

Downstream 100 bases:

>100_bases
CGTCTTATCATAATTAATTAAATTACCAAAAAGGTGCTCATTCTATTTAATACATTCGCTGAATTGCCCGAGGCTTATAA
AGCCTTTGCTCCAACTGTTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFEFEITSNCSNTAARTGIFHTPNGQVNTPKFMPVGTLATVKGISSKQLTSTGSEMILSNTFHLHLQPGEKLVKESGGIH
KFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDGSYVFLSPEKVIQIQMDLGSDVAMAFDHCPPHTANENDIED
SLQRTHSWLQKSVETHQKSNQALFGIVQGGKYPKLREYSAKYTSSFDLPGIAVGGVSVGETVEEIHSVINYVPKFLPIKK
PRYLMGIGSLKEISLAVANGFDIFDCVLPTRLGRHGTAFFNDERLNLRNARFKNDFSPIDKTCKCETCKSYSRAYLHHLI
RNDEILGLTLISLHNIAHLIRFTNAISTAIRDNCFTNDFAPWKTSSIAHHTW

Sequences:

>Translated_372_residues
MFEFEITSNCSNTAARTGIFHTPNGQVNTPKFMPVGTLATVKGISSKQLTSTGSEMILSNTFHLHLQPGEKLVKESGGIH
KFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDGSYVFLSPEKVIQIQMDLGSDVAMAFDHCPPHTANENDIED
SLQRTHSWLQKSVETHQKSNQALFGIVQGGKYPKLREYSAKYTSSFDLPGIAVGGVSVGETVEEIHSVINYVPKFLPIKK
PRYLMGIGSLKEISLAVANGFDIFDCVLPTRLGRHGTAFFNDERLNLRNARFKNDFSPIDKTCKCETCKSYSRAYLHHLI
RNDEILGLTLISLHNIAHLIRFTNAISTAIRDNCFTNDFAPWKTSSIAHHTW
>Mature_372_residues
MFEFEITSNCSNTAARTGIFHTPNGQVNTPKFMPVGTLATVKGISSKQLTSTGSEMILSNTFHLHLQPGEKLVKESGGIH
KFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDGSYVFLSPEKVIQIQMDLGSDVAMAFDHCPPHTANENDIED
SLQRTHSWLQKSVETHQKSNQALFGIVQGGKYPKLREYSAKYTSSFDLPGIAVGGVSVGETVEEIHSVINYVPKFLPIKK
PRYLMGIGSLKEISLAVANGFDIFDCVLPTRLGRHGTAFFNDERLNLRNARFKNDFSPIDKTCKCETCKSYSRAYLHHLI
RNDEILGLTLISLHNIAHLIRFTNAISTAIRDNCFTNDFAPWKTSSIAHHTW

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=41.6901408450704, Blast_Score=315, Evalue=4e-86,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=43.5261707988981, Blast_Score=326, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17544680, Length=340, Percent_Identity=42.0588235294118, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=229, Percent_Identity=25.3275109170306, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920510, Length=353, Percent_Identity=40.7932011331445, Blast_Score=308, Evalue=3e-84,
Organism=Drosophila melanogaster, GI21355311, Length=247, Percent_Identity=25.1012145748988, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41525; Mature: 41525

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEFEITSNCSNTAARTGIFHTPNGQVNTPKFMPVGTLATVKGISSKQLTSTGSEMILSN
CEEEEECCCCCCHHHHCCEEECCCCCCCCCCCCCCCHHHHHCCCCCHHHHCCCCCEEEEC
TFHLHLQPGEKLVKESGGIHKFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDG
EEEEEECCHHHHHHHCCCCHHHHCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCC
SYVFLSPEKVIQIQMDLGSDVAMAFDHCPPHTANENDIEDSLQRTHSWLQKSVETHQKSN
CEEEECCCEEEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
QALFGIVQGGKYPKLREYSAKYTSSFDLPGIAVGGVSVGETVEEIHSVINYVPKFLPIKK
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCC
PRYLMGIGSLKEISLAVANGFDIFDCVLPTRLGRHGTAFFNDERLNLRNARFKNDFSPID
CCEEEECCCHHHEEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
KTCKCETCKSYSRAYLHHLIRNDEILGLTLISLHNIAHLIRFTNAISTAIRDNCFTNDFA
CCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PWKTSSIAHHTW
CCCCCCCCCCCC
>Mature Secondary Structure
MFEFEITSNCSNTAARTGIFHTPNGQVNTPKFMPVGTLATVKGISSKQLTSTGSEMILSN
CEEEEECCCCCCHHHHCCEEECCCCCCCCCCCCCCCHHHHHCCCCCHHHHCCCCCEEEEC
TFHLHLQPGEKLVKESGGIHKFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDG
EEEEEECCHHHHHHHCCCCHHHHCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCC
SYVFLSPEKVIQIQMDLGSDVAMAFDHCPPHTANENDIEDSLQRTHSWLQKSVETHQKSN
CEEEECCCEEEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
QALFGIVQGGKYPKLREYSAKYTSSFDLPGIAVGGVSVGETVEEIHSVINYVPKFLPIKK
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCC
PRYLMGIGSLKEISLAVANGFDIFDCVLPTRLGRHGTAFFNDERLNLRNARFKNDFSPID
CCEEEECCCHHHEEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
KTCKCETCKSYSRAYLHHLIRNDEILGLTLISLHNIAHLIRFTNAISTAIRDNCFTNDFA
CCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PWKTSSIAHHTW
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA