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The map label for this gene is grpE [H]

Identifier: 123967552

GI number: 123967552

Start: 18225

End: 18944

Strand: Direct

Name: grpE [H]

Synonym: A9601_00151

Alternate gene names: 123967552

Gene position: 18225-18944 (Clockwise)

Preceding gene: 123967551

Following gene: 123967553

Centisome position: 1.09

GC content: 31.39

Gene sequence:

>720_bases
ATGATTGAAAATCAATCAGACAATATTGATAATAAAGAAAATGATCTTTCTGATCAGGATAATGCTCTTGAAAATGTATC
ATCTGCGCAAGAATTAACTACTGAAAATAATGAATTATCTTCTCAAAAAACAGAAGAAATAAATACCGAAGAATTAAAAA
ATTCTATTTCTAATAATGATGCAAGATTAGAGCAATTAGAAAAAGAGCATGAAACATTAAAAAATCAATATGTAAGGATT
TCAGCAGATTTTGATAATTTTAGAAAAAGGCAGTCTAGAGATCAGGATGATTTAAAAATTCAACTTGTTTCAAAGACTTT
AACTGCAATACTACCTATTGTTGATAACTTCGAGAGAGCAAGACAACAACTAAAACCTGAAAGTGAAGAAGCTCAATCTC
TTCATAGAAGTTATCAAGGATTATATAAACAATTGGTAGAAGTTTTGAAACAACAGGGAGTTTCCCCCATGAGAGTTGTT
GGTCAGCAATTTGACCCAAGCTTGCATGAAGCTGTATTAAGAGAGCCTAGTGAAAAGTTTGAAGAAGATTTTATAATTGA
AGAATTGCAGCGAGGATATCATCTTGAAGGTAAGGTTTTGAGACATGCTTTGGTAAAAGTTTCTATGGGACCTGGAAAAC
AAAATTCACAAGAGGAAGTAGAAAAGGATAAAGTTGAAGGGGATATTGATTCAGAGGAAAATACTTCTGAAGATGTATAA

Upstream 100 bases:

>100_bases
TAGCATTAAGGTTTGTTCCTGACTAAATATTTGTGCGGCCTTCCGCAACTTGTTTTGTGCATCACTTTGTTGGAGAATAG
TTTTATTAAATTTTAGAAAA

Downstream 100 bases:

>100_bases
ATTCCAAATTTTTACTTAAGCATTATCTAATGGCTGATTTCTATCAAATACTTGGAGTTTCAAGAGATGCTGATGCAGAT
ACCTTAAAAAAGGCTTATAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIENQSDNIDNKENDLSDQDNALENVSSAQELTTENNELSSQKTEEINTEELKNSISNNDARLEQLEKEHETLKNQYVRI
SADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVV
GQQFDPSLHEAVLREPSEKFEEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQEEVEKDKVEGDIDSEENTSEDV

Sequences:

>Translated_239_residues
MIENQSDNIDNKENDLSDQDNALENVSSAQELTTENNELSSQKTEEINTEELKNSISNNDARLEQLEKEHETLKNQYVRI
SADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVV
GQQFDPSLHEAVLREPSEKFEEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQEEVEKDKVEGDIDSEENTSEDV
>Mature_239_residues
MIENQSDNIDNKENDLSDQDNALENVSSAQELTTENNELSSQKTEEINTEELKNSISNNDARLEQLEKEHETLKNQYVRI
SADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVV
GQQFDPSLHEAVLREPSEKFEEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQEEVEKDKVEGDIDSEENTSEDV

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Drosophila melanogaster, GI24653432, Length=140, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 27528; Mature: 27528

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENQSDNIDNKENDLSDQDNALENVSSAQELTTENNELSSQKTEEINTEELKNSISNND
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCH
ARLEQLEKEHETLKNQYVRISADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERA
HHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPSLHEAVLREPSEKF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCHHHHH
EEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQEEVEKDKVEGDIDSEENTSEDV
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIENQSDNIDNKENDLSDQDNALENVSSAQELTTENNELSSQKTEEINTEELKNSISNND
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCH
ARLEQLEKEHETLKNQYVRISADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERA
HHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPSLHEAVLREPSEKF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCHHHHH
EEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQEEVEKDKVEGDIDSEENTSEDV
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA