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The map label for this gene is rpoD4 [H]

Identifier: 123967121

GI number: 123967121

Start: 1678896

End: 1679915

Strand: Direct

Name: rpoD4 [H]

Synonym: P9515_18881

Alternate gene names: 123967121

Gene position: 1678896-1679915 (Clockwise)

Preceding gene: 123967120

Following gene: 123967122

Centisome position: 98.52

GC content: 36.96

Gene sequence:

>1020_bases
ATGGGGATCCTTCTGGAATCTGGAAATAGTTCTTCAAAAAATAATAATGAAGAACCTAGATTACCAAACACTGCGGGCAA
GACTCGCAAAACAAAATCCAGCTTAAGTGCTAAACAAAGCCAAAAAAAATCTGCAAGACTTGCTTCAGATTCTATTGGTT
ATTACTTAAGCAGTATTGGAAGAGTTCCTCTGCTTACTGCCGCAGAGGAAATAGAGCTAGCACATCATGTTCAAAACATG
AAAAAAATGCTAAAAATTCCTGAAATTGAAAGATCATCAAGAAATCGTCATCTTATTAAAGTTGGGAAGAGGGCTAGAGA
TAGAATGATGTCTGCCAATTTAAGACTTGTAGTATCTGTTGCAAAAAAGTATCAAAATCAAGGTTTAGAACTATTAGACT
TGGTACAAGAAGGCGCTATTGGCTTAGAGAGGGCTGTTGATAAATTTGATCCTGCAATGGGATATAAATTTTCTACTTAT
GCTTATTGGTGGATTAGACAAGGAATGACAAGAGCTATTGATAATAGTGCTAGAACAATCCGATTGCCAATTCATATAAG
TGAAAAATTATCCAAAATGAGGAGAGTTTCCCGAGAATTATCTCAAAAATCTGGAAGGCAACCAACCAGATTAGAAATGG
CAACAGCGATGGGGATTGATCAAAAAGATTTAGAAGACTTAATCTCTCAAAGTGCTCCATGCGCATCTCTTGACGCTCAT
GCAAGAGGAGAAGAAGATCGTAGTACTCTAGGTGAACTTATTCCAGATCCAAATGGTGAAGAGCCAATGGAAGGAATGGA
TAGAACTATCCAAAAAGAACATTTAGGAACTTGGCTAACTCAGCTAAATGAGAGGGAACAAAAAATCATGAAACTAAGAT
TCGGCTTAGACGGAGAAGAGCCACTAACTCTGGCCGAAATAGGAAGACAAATAAATGTATCTCGAGAAAGAGTTAGACAA
CTAGAAGCAAAGGCAATACTAAAATTAAGAGTAATGACAACTCATCAAAAAGCAGCATAA

Upstream 100 bases:

>100_bases
AAATCTTCCTAATTTGTCTTTAAAGTTTCAACGCAAAAGTAGTTTTTATCTCGATTTCAGTGCTAGCTTTTTAAAAAATC
CGTTTTAAGAGGCCAGGGTG

Downstream 100 bases:

>100_bases
TTAAGTAGTTGAAATTTGTTTTTCTTTCTAGTTATATATTAATTGTCTTTTTAATATCAATAATTTATAAAAGGTTTGAT
CAGGATAATAAAGAGGCACT

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGILLESGNSSSKNNNEEPRLPNTAGKTRKTKSSLSAKQSQKKSARLASDSIGYYLSSIGRVPLLTAAEEIELAHHVQNM
KKMLKIPEIERSSRNRHLIKVGKRARDRMMSANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTY
AYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRVSRELSQKSGRQPTRLEMATAMGIDQKDLEDLISQSAPCASLDAH
ARGEEDRSTLGELIPDPNGEEPMEGMDRTIQKEHLGTWLTQLNEREQKIMKLRFGLDGEEPLTLAEIGRQINVSRERVRQ
LEAKAILKLRVMTTHQKAA

Sequences:

>Translated_339_residues
MGILLESGNSSSKNNNEEPRLPNTAGKTRKTKSSLSAKQSQKKSARLASDSIGYYLSSIGRVPLLTAAEEIELAHHVQNM
KKMLKIPEIERSSRNRHLIKVGKRARDRMMSANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTY
AYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRVSRELSQKSGRQPTRLEMATAMGIDQKDLEDLISQSAPCASLDAH
ARGEEDRSTLGELIPDPNGEEPMEGMDRTIQKEHLGTWLTQLNEREQKIMKLRFGLDGEEPLTLAEIGRQINVSRERVRQ
LEAKAILKLRVMTTHQKAA
>Mature_338_residues
GILLESGNSSSKNNNEEPRLPNTAGKTRKTKSSLSAKQSQKKSARLASDSIGYYLSSIGRVPLLTAAEEIELAHHVQNMK
KMLKIPEIERSSRNRHLIKVGKRARDRMMSANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYA
YWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRVSRELSQKSGRQPTRLEMATAMGIDQKDLEDLISQSAPCASLDAHA
RGEEDRSTLGELIPDPNGEEPMEGMDRTIQKEHLGTWLTQLNEREQKIMKLRFGLDGEEPLTLAEIGRQINVSRERVRQL
EAKAILKLRVMTTHQKAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=45.4148471615721, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=37.9790940766551, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788231, Length=208, Percent_Identity=29.8076923076923, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38268; Mature: 38136

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILLESGNSSSKNNNEEPRLPNTAGKTRKTKSSLSAKQSQKKSARLASDSIGYYLSSIG
CCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RVPLLTAAEEIELAHHVQNMKKMLKIPEIERSSRNRHLIKVGKRARDRMMSANLRLVVSV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
AKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYAYWWIRQGMTRAIDNSARTI
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
RLPIHISEKLSKMRRVSRELSQKSGRQPTRLEMATAMGIDQKDLEDLISQSAPCASLDAH
EEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
ARGEEDRSTLGELIPDPNGEEPMEGMDRTIQKEHLGTWLTQLNEREQKIMKLRFGLDGEE
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLTLAEIGRQINVSRERVRQLEAKAILKLRVMTTHQKAA
CEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GILLESGNSSSKNNNEEPRLPNTAGKTRKTKSSLSAKQSQKKSARLASDSIGYYLSSIG
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RVPLLTAAEEIELAHHVQNMKKMLKIPEIERSSRNRHLIKVGKRARDRMMSANLRLVVSV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
AKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYAYWWIRQGMTRAIDNSARTI
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
RLPIHISEKLSKMRRVSRELSQKSGRQPTRLEMATAMGIDQKDLEDLISQSAPCASLDAH
EEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
ARGEEDRSTLGELIPDPNGEEPMEGMDRTIQKEHLGTWLTQLNEREQKIMKLRFGLDGEE
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLTLAEIGRQINVSRERVRQLEAKAILKLRVMTTHQKAA
CEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA