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The map label for this gene is gyrB [H]

Identifier: 123967056

GI number: 123967056

Start: 1605027

End: 1606994

Strand: Reverse

Name: gyrB [H]

Synonym: P9515_18231

Alternate gene names: 123967056

Gene position: 1606994-1605027 (Counterclockwise)

Preceding gene: 123967061

Following gene: 123967055

Centisome position: 94.3

GC content: 34.35

Gene sequence:

>1968_bases
ATGAGTGAGGAAAAAAGATCTAATAAAATCTCTAATGATTATGGTGCTGAACAGATACAGGTCTTAGAGGGGTTAGAACC
TGTTCGTAAAAGACCAGGTATGTACATAGGTTCGACCGGTCCAAGGGGGTTGCATCATTTAGTATATGAAGTAGTAGATA
ATTCAGTAGATGAAGCACTAGCTGGGCATTGTGATCAAATTGAAATAGTTCTCAAAGATGATGGATCAGCTTCCATTTCT
GATAATGGAAGGGGTATCCCTACCGATATACATCCAAGAACTGGAAAGAGCGCTCTAGAAACTGTGCTTACTGTTCTTCA
TGCAGGGGGTAAATTTGGCAGTGGTGGATATAAAGTTTCCGGTGGTTTACATGGTGTTGGTATTTCTGTGGTTAATGCTT
TAAGTGAGTGGGTTAATGTTACTGTCTTTAGAGAAGGAAGTGAGTTTACTCAAAGATTCGAGAGAGGTATAACAAAAGGG
GAATTAAAAACTCAGAAGCAGCAGAATAAATCTTTAAGAAAAGGTACGATAATTTGTTTTAAGCCAGATACAACTATTTT
TACTGATGGGATTGAATTTGATTATGCTCTTTTATCTTCAAGATTACGAGAATTAGCTTATCTTAACGGCGGCGTCAAAA
TTATATTTATAGATGAGAGACAAAAATTACCAGATGGTTCTTTCAAAGAAGAAATTTACTTATATGATGGTGGCATTAAA
GAGTATGTTCAATATATCAATAAAGAAAAGGAATCGATTCATTCAGAGATTATTTATGTTGACTCTCAAAAAGATAATGT
TTATGTAGAGGCAGCTCTTCAATGGTGTTCAGATGTATATTCAGATAACATTTTAGGATTTGCAAATAATATCAGAACTA
TTGACGGAGGCACACATATAGAAGGTTTAAAAACAGTATTGACAAGAACCTTTAATGCCATTGCTAAAAAAAGAGGTAAA
CGAAAGGAAGTTGACAAAAATTTATCAGGAGAAAATATCAGAGAAGGGTTGACTGTCGTGTTATCTGTAAAAGTTCCAGA
TCCAGAATTTGAAGGACAAACTAAGACAAAATTGGGAAACACTGAAGTAAGAGGAATAGTGGATTCGCTCATAGGCGAAT
CACTAACAAAATATATGGAATTCAATCCTAATGTTTTCGATTTGATTTTAGAAAAAGCAATTCAATCTTTTAATGCAGCA
GAGGCAGCGAAACGAGCAAGGGATTTAGTAAGGAGAAAAAGTGTTCTTGAGAGCTCTACTTTGCCTGGTAAATTGGCAGA
TTGTAGCTCAAGAGATCCTTCAGGCTCAGAGATTTATATAGTTGAGGGTGATTCTGCTGGAGGTTCAGCTAAACAAGGAA
GAGATAGAAAATTCCAAGCAATTTTGCCTTTGAGAGGCAAAATATTAAATATTGAAAAAACTGATGATACTAAAATATAT
AAAAATACAGAAATTCAATCACTAATAACTGCTTTGGGCCTTGGCATTAAGGGTGAAGAGTTTGATGTAAATTCTCTAAG
ATATCATCGTGTTGTGATAATGACTGATGCTGATGTCGACGGAGCTCATATAAGAACACTTCTACTAACGTTTTTTTATA
GATATCAAAGAGAACTGGTTGAAAAAGGTTTCATATATATTGCCTGTCCGCCATTATATAAAGTGGAAAGGGGTAAAAAT
CATAAATATTGTTATAACGAAGGTCAATTGAAAAAAACCATTGAAGACTTTGGAGAAAAAGCTAACTACAATATCCAAAG
ATTTAAAGGATTAGGTGAGATGATGCCAAAACAATTATGGGATACAACCATGAATCCTGAGACAAGAATGATGAAAAAAG
TAGAAATAGAAGATGCTTTAGAAGCAGATCGAATATTTAATATTCTAATGGGAGATAAAGTAGCACCAAGAAGAGAATTT
ATAGAAACTCATAGTGCCAATCTAGATATAGCAACATTAGATATATGA

Upstream 100 bases:

>100_bases
TGCATTTACTTCAAGCCTTTCTCTTATACCGATGGTAAATTTAATGAAAGGCCTTTTAAAAATAACATTTTAAAAGTTTT
ATTATTTATTCTTATATTAA

Downstream 100 bases:

>100_bases
TAAAAAATATGATAAAAAATATTTGTTTAATAGGATTTGCTTTAATTGGCCCCATAACTCTTCCTGCAGGGGGAATTCAA
AGGAATTTAAATGAATATAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSEEKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDQIEIVLKDDGSASIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVFREGSEFTQRFERGITKG
ELKTQKQQNKSLRKGTIICFKPDTTIFTDGIEFDYALLSSRLRELAYLNGGVKIIFIDERQKLPDGSFKEEIYLYDGGIK
EYVQYINKEKESIHSEIIYVDSQKDNVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNAIAKKRGK
RKEVDKNLSGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGESLTKYMEFNPNVFDLILEKAIQSFNAA
EAAKRARDLVRRKSVLESSTLPGKLADCSSRDPSGSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDVNSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKN
HKYCYNEGQLKKTIEDFGEKANYNIQRFKGLGEMMPKQLWDTTMNPETRMMKKVEIEDALEADRIFNILMGDKVAPRREF
IETHSANLDIATLDI

Sequences:

>Translated_655_residues
MSEEKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDQIEIVLKDDGSASIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVFREGSEFTQRFERGITKG
ELKTQKQQNKSLRKGTIICFKPDTTIFTDGIEFDYALLSSRLRELAYLNGGVKIIFIDERQKLPDGSFKEEIYLYDGGIK
EYVQYINKEKESIHSEIIYVDSQKDNVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNAIAKKRGK
RKEVDKNLSGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGESLTKYMEFNPNVFDLILEKAIQSFNAA
EAAKRARDLVRRKSVLESSTLPGKLADCSSRDPSGSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDVNSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKN
HKYCYNEGQLKKTIEDFGEKANYNIQRFKGLGEMMPKQLWDTTMNPETRMMKKVEIEDALEADRIFNILMGDKVAPRREF
IETHSANLDIATLDI
>Mature_654_residues
SEEKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDQIEIVLKDDGSASISD
NGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVFREGSEFTQRFERGITKGE
LKTQKQQNKSLRKGTIICFKPDTTIFTDGIEFDYALLSSRLRELAYLNGGVKIIFIDERQKLPDGSFKEEIYLYDGGIKE
YVQYINKEKESIHSEIIYVDSQKDNVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNAIAKKRGKR
KEVDKNLSGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGESLTKYMEFNPNVFDLILEKAIQSFNAAE
AAKRARDLVRRKSVLESSTLPGKLADCSSRDPSGSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIYK
NTEIQSLITALGLGIKGEEFDVNSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKNH
KYCYNEGQLKKTIEDFGEKANYNIQRFKGLGEMMPKQLWDTTMNPETRMMKKVEIEDALEADRIFNILMGDKVAPRREFI
ETHSANLDIATLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=643, Percent_Identity=24.8833592534992, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19913406, Length=624, Percent_Identity=22.9166666666667, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=52.5939177101968, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1789408, Length=637, Percent_Identity=39.0894819466248, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI212645845, Length=566, Percent_Identity=25.7950530035336, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535065, Length=577, Percent_Identity=24.9566724436742, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=27.7511961722488, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=633, Percent_Identity=26.3823064770932, Blast_Score=136, Evalue=9e-33,
Organism=Drosophila melanogaster, GI17136538, Length=574, Percent_Identity=26.3066202090592, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73389; Mature: 73258

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEAL
CCCHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDQIEIVLKDDGSASISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVNVTVFREGSEFTQRFERGITKGELKTQKQQNKSLRKGTIICF
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEE
KPDTTIFTDGIEFDYALLSSRLRELAYLNGGVKIIFIDERQKLPDGSFKEEIYLYDGGIK
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCHH
EYVQYINKEKESIHSEIIYVDSQKDNVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCEEECCCCEECCCCCCH
EGLKTVLTRTFNAIAKKRGKRKEVDKNLSGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGESLTKYMEFNPNVFDLILEKAIQSFNAAEAAKRARDLVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSGSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDVNSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGFIYIACPPLYKVERGKNHKYCYNEGQLKKTIEDFGEKANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
DTTMNPETRMMKKVEIEDALEADRIFNILMGDKVAPRREFIETHSANLDIATLDI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
SEEKRSNKISNDYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEAL
CCHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDQIEIVLKDDGSASISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVNVTVFREGSEFTQRFERGITKGELKTQKQQNKSLRKGTIICF
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEE
KPDTTIFTDGIEFDYALLSSRLRELAYLNGGVKIIFIDERQKLPDGSFKEEIYLYDGGIK
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCHH
EYVQYINKEKESIHSEIIYVDSQKDNVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCEEECCCCEECCCCCCH
EGLKTVLTRTFNAIAKKRGKRKEVDKNLSGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGESLTKYMEFNPNVFDLILEKAIQSFNAAEAAKRARDLVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSGSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDVNSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGFIYIACPPLYKVERGKNHKYCYNEGQLKKTIEDFGEKANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
DTTMNPETRMMKKVEIEDALEADRIFNILMGDKVAPRREFIETHSANLDIATLDI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]