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The map label for this gene is rpoD2 [H]

Identifier: 123967051

GI number: 123967051

Start: 1601446

End: 1602447

Strand: Reverse

Name: rpoD2 [H]

Synonym: P9515_18181

Alternate gene names: 123967051

Gene position: 1602447-1601446 (Counterclockwise)

Preceding gene: 123967052

Following gene: 123967049

Centisome position: 94.03

GC content: 36.43

Gene sequence:

>1002_bases
ATGTCATCCGAAACATTAGTTGAGAATAAATTAGCTAATATATCTTCTCTCAAATCTAGCAACGATGTAGATTTGGTAAG
GTCATATTTGAGAGATATTGGAAGAGTTCCACTACTATCACATGAGCAAGAAATAACTCTAGGGAGACAAGTTCAAGAAT
ACATGCAAGTTGAGAGGGCTGAGCTTGAAATCATAGAATTGACAGATAATAAACCCTCAGTTGAAGAATTAGCCGCAAAA
TTAAATTTATCTTCATCACAAATTAAAAAAAGACTCAGAGCTGGTCAACGTGCGAAAGAAAGAATGGTTGCTGCAAATCT
AAGACTTGTTGTTAGTGTTGCCAAAAAATATACTAAGAGAAATATGGAACTACTTGATTTAATTCAAGAAGGAACAATTG
GATTGGTAAGGGGGGTAGAAAAATTCGATCCTGCAAGAGGATATAAATTTTCAACTTATGCCTATTGGTGGATTAGGCAA
GGAATTACTAGAGCAATTGCGGAAAAAAGTAGAGCTATTAGATTACCTATTCATATCACCGAAATGCTTAATAAGTTAAA
AAAAGGGCAACGAGAATTGAGTCAAGAAATGTCAAGAACGCCTACAATTAGCGAACTTGCGAAATATGTTGAATTACCTG
AAGATGATGTTAAGGATTTAATGTGCAAAGCGGGCCAGCCAGTGAGTCTCGAAACTAAGGTTGGTGATGGCGAAGATACA
GTATTACTAGATTTACTAGCTGGAGGAGAGGATTTGCCTGACGAACAAATTGAGATGGATTGTATGAGAGGTGATCTTCA
TTCTCTACTTCATCAGCTGCCTGATCTTCAATGTAGAGTTTTAAGAATGAGATATGGAATGGATGGAGATGAACCAATGT
CTCTAACTGGTATTGGACGTGTTTTAGGTATCAGTAGAGATAGAGTAAGAAATTTAGAAAGAGATGGTTTGAGAGGTTTA
CGTAGACTCAGTGAGAATGTTGAAGCCTATTTCGTTTCTTGA

Upstream 100 bases:

>100_bases
AAACCACTAGGTATTTATTCTCATTTTTGTAAATTTGTGGATTTTTTTGTTTAACTTTACATTTCTTAATGGTATTGTTT
ACAAAACAAGATTAACTTTT

Downstream 100 bases:

>100_bases
ATAAATTGATTTATTAAATTGTTAGTTTCCTCAGGATTTTCATCATGTGGACAATGTCCGACTTCCTTAATAACATCAAG
TCTCTTAATATTTTTATATT

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSSETLVENKLANISSLKSSNDVDLVRSYLRDIGRVPLLSHEQEITLGRQVQEYMQVERAELEIIELTDNKPSVEELAAK
LNLSSSQIKKRLRAGQRAKERMVAANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPARGYKFSTYAYWWIRQ
GITRAIAEKSRAIRLPIHITEMLNKLKKGQRELSQEMSRTPTISELAKYVELPEDDVKDLMCKAGQPVSLETKVGDGEDT
VLLDLLAGGEDLPDEQIEMDCMRGDLHSLLHQLPDLQCRVLRMRYGMDGDEPMSLTGIGRVLGISRDRVRNLERDGLRGL
RRLSENVEAYFVS

Sequences:

>Translated_333_residues
MSSETLVENKLANISSLKSSNDVDLVRSYLRDIGRVPLLSHEQEITLGRQVQEYMQVERAELEIIELTDNKPSVEELAAK
LNLSSSQIKKRLRAGQRAKERMVAANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPARGYKFSTYAYWWIRQ
GITRAIAEKSRAIRLPIHITEMLNKLKKGQRELSQEMSRTPTISELAKYVELPEDDVKDLMCKAGQPVSLETKVGDGEDT
VLLDLLAGGEDLPDEQIEMDCMRGDLHSLLHQLPDLQCRVLRMRYGMDGDEPMSLTGIGRVLGISRDRVRNLERDGLRGL
RRLSENVEAYFVS
>Mature_332_residues
SSETLVENKLANISSLKSSNDVDLVRSYLRDIGRVPLLSHEQEITLGRQVQEYMQVERAELEIIELTDNKPSVEELAAKL
NLSSSQIKKRLRAGQRAKERMVAANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPARGYKFSTYAYWWIRQG
ITRAIAEKSRAIRLPIHITEMLNKLKKGQRELSQEMSRTPTISELAKYVELPEDDVKDLMCKAGQPVSLETKVGDGEDTV
LLDLLAGGEDLPDEQIEMDCMRGDLHSLLHQLPDLQCRVLRMRYGMDGDEPMSLTGIGRVLGISRDRVRNLERDGLRGLR
RLSENVEAYFVS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=45.4545454545455, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1789098, Length=314, Percent_Identity=36.624203821656, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789871, Length=249, Percent_Identity=23.6947791164659, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37812; Mature: 37681

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSETLVENKLANISSLKSSNDVDLVRSYLRDIGRVPLLSHEQEITLGRQVQEYMQVERA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELEIIELTDNKPSVEELAAKLNLSSSQIKKRLRAGQRAKERMVAANLRLVVSVAKKYTKR
HEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMELLDLIQEGTIGLVRGVEKFDPARGYKFSTYAYWWIRQGITRAIAEKSRAIRLPIHIT
CHHHHHHHHHCCHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHH
EMLNKLKKGQRELSQEMSRTPTISELAKYVELPEDDVKDLMCKAGQPVSLETKVGDGEDT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCC
VLLDLLAGGEDLPDEQIEMDCMRGDLHSLLHQLPDLQCRVLRMRYGMDGDEPMSLTGIGR
EEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
VLGISRDRVRNLERDGLRGLRRLSENVEAYFVS
HHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
SSETLVENKLANISSLKSSNDVDLVRSYLRDIGRVPLLSHEQEITLGRQVQEYMQVERA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELEIIELTDNKPSVEELAAKLNLSSSQIKKRLRAGQRAKERMVAANLRLVVSVAKKYTKR
HEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMELLDLIQEGTIGLVRGVEKFDPARGYKFSTYAYWWIRQGITRAIAEKSRAIRLPIHIT
CHHHHHHHHHCCHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHH
EMLNKLKKGQRELSQEMSRTPTISELAKYVELPEDDVKDLMCKAGQPVSLETKVGDGEDT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCC
VLLDLLAGGEDLPDEQIEMDCMRGDLHSLLHQLPDLQCRVLRMRYGMDGDEPMSLTGIGR
EEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
VLGISRDRVRNLERDGLRGLRRLSENVEAYFVS
HHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]