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The map label for this gene is trpE [H]

Identifier: 123966997

GI number: 123966997

Start: 1551143

End: 1552663

Strand: Reverse

Name: trpE [H]

Synonym: P9515_17641

Alternate gene names: 123966997

Gene position: 1552663-1551143 (Counterclockwise)

Preceding gene: 123966998

Following gene: 123966996

Centisome position: 91.11

GC content: 33.4

Gene sequence:

>1521_bases
ATGAGCAGTTCTCAAAAAGATATTTTTTATAAGGCTTATAAAGAAGGTAAAAATTTTATACCTCTTATCAAAACTTGGCC
TGCAGATTTAGAGACCCCATTATCAACATGGCTGAAACTTTCAAATGAAAATTCACATGGTGTCTTTCTTGAATCGGTTG
AAGGTGGAGAAAATGTTGGCAGATGGAGCATCGTAGCTGTTAATCCTCTGTGGGAAGCTGTTTGTCATGGTAAAAAAACT
ATTAAAACTTGGAGTTGTGGAAAAAGTACAATATACAATGAAGATCCTTTTGATTTATTAAAAACTTGGACAGAAGAATA
TAAGTCTTATAGTTTAGAAAATTTACCTTACATTGGACAGTTATATGGTTCTTGGGGCTATGAATTAATTAATTATGTAG
AACCTAATGTTCCTATTAATGAGTTAGAGGAGAAAGAAATACCCTATGGTTCTTGGATGTTTTTTGATCAATTAGTAATA
TTTGATCAAATGAAAAGATGTATAACTGCAGTAGTCTATGCTGATACCGCTAGCGCATCTGCATCTAATATTGAGAGAAT
TTATAAAGATTCTATTTTAAAAATTGATACAATTCGGGATTTAATGAGACTTCCACTTAAAGAAACAGAGGTTCTAAATT
GGAATGAAAGTAGAGACTTGAATATTGAAATTTCAAGTAATTGGAATAAAAAAGAATTTGAAGATGCAGTCGTTTCTGCA
AAAGAATATATTAAAAAAGGTGATATTTTTCAGATTGTTATAAGTCAAAAATTTCAAGCGAAGGTTAAAAGTGATCCCTT
CAATCTATATAGGAGTTTAAGGATGGTTAATCCTTCTCCTTATATGGCATTCTTTGATTTTGGAACATGGTATCTCATAG
GTTCTAGTCCTGAAGTAATGGTTAAAGCAGAGAAGAATAAAAACAATCAAATTGTTGCTAGCCTTCGACCTATAGCTGGG
ACTAGACCAAAAGGTAAAGATGCAGAACAAGATCTGAAGTTTGAGGAAGAGTTATTAAGTGATCCAAAAGAGATATCTGA
ACATGTAATGCTTATTGATCTTGGAAGAAATGATTTAGGAAGAGTATGCGAAACGGGTACGGTAGAGGTTAAAGATTTGA
TGATAATCGAAAAGTATTCACATGTTATGCACATAGTAAGCGAAGTTGAGGGTGTTTTGAAAAAGAATAAAGGAGTTTGG
GATTTGTTTAAAGCATGTTTTCCTGCTGGTACAGTAACTGGGGCACCAAAAATAAGAGCTATGCAGCTAATTAAGAATTT
TGAAAAAGATGCTAGAGGGCCATATGCTGGTGTTTATGGATCTATAGATATAAATGGTGCACTAAATACTGCAATAACGA
TAAGAACAATGATTGTTAAACCCTCGAATGAAGGGGAATATACAGTTTCTGTTCAAGCTGGCGCTGGGATAGTCGCAGAT
TCATCTCCTGTGAACGAATATCAAGAGACCATCAATAAAGCTAAAGGAATATTGATGTCATTGGCTTGTTTGGATAGATA
A

Upstream 100 bases:

>100_bases
CTGCAGGTTTAAAATTTACAACTAAAAAAACTTTTGAATAAATTTTCGAAAGTTGATATGAAAGACAAATATAATTATAA
TTTGGGCTGAAATTATTAGT

Downstream 100 bases:

>100_bases
ATGCATAATTATAATTTGTATAAAGGCTTTGAAGTCGAACTCTTTACTGGTTCTAAAAATAATCATATTGGAGTTTCTGA
TGAGATAGAAAAAACATTTT

Product: anthranilate synthase component I/chorismate-binding protein

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSSSQKDIFYKAYKEGKNFIPLIKTWPADLETPLSTWLKLSNENSHGVFLESVEGGENVGRWSIVAVNPLWEAVCHGKKT
IKTWSCGKSTIYNEDPFDLLKTWTEEYKSYSLENLPYIGQLYGSWGYELINYVEPNVPINELEEKEIPYGSWMFFDQLVI
FDQMKRCITAVVYADTASASASNIERIYKDSILKIDTIRDLMRLPLKETEVLNWNESRDLNIEISSNWNKKEFEDAVVSA
KEYIKKGDIFQIVISQKFQAKVKSDPFNLYRSLRMVNPSPYMAFFDFGTWYLIGSSPEVMVKAEKNKNNQIVASLRPIAG
TRPKGKDAEQDLKFEEELLSDPKEISEHVMLIDLGRNDLGRVCETGTVEVKDLMIIEKYSHVMHIVSEVEGVLKKNKGVW
DLFKACFPAGTVTGAPKIRAMQLIKNFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSNEGEYTVSVQAGAGIVAD
SSPVNEYQETINKAKGILMSLACLDR

Sequences:

>Translated_506_residues
MSSSQKDIFYKAYKEGKNFIPLIKTWPADLETPLSTWLKLSNENSHGVFLESVEGGENVGRWSIVAVNPLWEAVCHGKKT
IKTWSCGKSTIYNEDPFDLLKTWTEEYKSYSLENLPYIGQLYGSWGYELINYVEPNVPINELEEKEIPYGSWMFFDQLVI
FDQMKRCITAVVYADTASASASNIERIYKDSILKIDTIRDLMRLPLKETEVLNWNESRDLNIEISSNWNKKEFEDAVVSA
KEYIKKGDIFQIVISQKFQAKVKSDPFNLYRSLRMVNPSPYMAFFDFGTWYLIGSSPEVMVKAEKNKNNQIVASLRPIAG
TRPKGKDAEQDLKFEEELLSDPKEISEHVMLIDLGRNDLGRVCETGTVEVKDLMIIEKYSHVMHIVSEVEGVLKKNKGVW
DLFKACFPAGTVTGAPKIRAMQLIKNFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSNEGEYTVSVQAGAGIVAD
SSPVNEYQETINKAKGILMSLACLDR
>Mature_505_residues
SSSQKDIFYKAYKEGKNFIPLIKTWPADLETPLSTWLKLSNENSHGVFLESVEGGENVGRWSIVAVNPLWEAVCHGKKTI
KTWSCGKSTIYNEDPFDLLKTWTEEYKSYSLENLPYIGQLYGSWGYELINYVEPNVPINELEEKEIPYGSWMFFDQLVIF
DQMKRCITAVVYADTASASASNIERIYKDSILKIDTIRDLMRLPLKETEVLNWNESRDLNIEISSNWNKKEFEDAVVSAK
EYIKKGDIFQIVISQKFQAKVKSDPFNLYRSLRMVNPSPYMAFFDFGTWYLIGSSPEVMVKAEKNKNNQIVASLRPIAGT
RPKGKDAEQDLKFEEELLSDPKEISEHVMLIDLGRNDLGRVCETGTVEVKDLMIIEKYSHVMHIVSEVEGVLKKNKGVWD
LFKACFPAGTVTGAPKIRAMQLIKNFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSNEGEYTVSVQAGAGIVADS
SPVNEYQETINKAKGILMSLACLDR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=288, Percent_Identity=40.9722222222222, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1788114, Length=459, Percent_Identity=29.1938997821351, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786809, Length=298, Percent_Identity=24.496644295302, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=491, Percent_Identity=34.6232179226069, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324361, Length=493, Percent_Identity=24.5436105476673, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57249; Mature: 57118

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSQKDIFYKAYKEGKNFIPLIKTWPADLETPLSTWLKLSNENSHGVFLESVEGGENVG
CCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHEECCCCCCCEEEEECCCCCCCC
RWSIVAVNPLWEAVCHGKKTIKTWSCGKSTIYNEDPFDLLKTWTEEYKSYSLENLPYIGQ
CEEEEEECHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LYGSWGYELINYVEPNVPINELEEKEIPYGSWMFFDQLVIFDQMKRCITAVVYADTASAS
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCC
ASNIERIYKDSILKIDTIRDLMRLPLKETEVLNWNESRDLNIEISSNWNKKEFEDAVVSA
HHHHHHHHHHCCEEHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHH
KEYIKKGDIFQIVISQKFQAKVKSDPFNLYRSLRMVNPSPYMAFFDFGTWYLIGSSPEVM
HHHHCCCCEEEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCEE
VKAEKNKNNQIVASLRPIAGTRPKGKDAEQDLKFEEELLSDPKEISEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCEEEEECCCCCHH
RVCETGTVEVKDLMIIEKYSHVMHIVSEVEGVLKKNKGVWDLFKACFPAGTVTGAPKIRA
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHH
MQLIKNFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSNEGEYTVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEC
SSPVNEYQETINKAKGILMSLACLDR
CCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSSQKDIFYKAYKEGKNFIPLIKTWPADLETPLSTWLKLSNENSHGVFLESVEGGENVG
CCCCCHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHEECCCCCCCEEEEECCCCCCCC
RWSIVAVNPLWEAVCHGKKTIKTWSCGKSTIYNEDPFDLLKTWTEEYKSYSLENLPYIGQ
CEEEEEECHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LYGSWGYELINYVEPNVPINELEEKEIPYGSWMFFDQLVIFDQMKRCITAVVYADTASAS
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCC
ASNIERIYKDSILKIDTIRDLMRLPLKETEVLNWNESRDLNIEISSNWNKKEFEDAVVSA
HHHHHHHHHHCCEEHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHH
KEYIKKGDIFQIVISQKFQAKVKSDPFNLYRSLRMVNPSPYMAFFDFGTWYLIGSSPEVM
HHHHCCCCEEEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCEE
VKAEKNKNNQIVASLRPIAGTRPKGKDAEQDLKFEEELLSDPKEISEHVMLIDLGRNDLG
EEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCEEEEECCCCCHH
RVCETGTVEVKDLMIIEKYSHVMHIVSEVEGVLKKNKGVWDLFKACFPAGTVTGAPKIRA
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHH
MQLIKNFEKDARGPYAGVYGSIDINGALNTAITIRTMIVKPSNEGEYTVSVQAGAGIVAD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEC
SSPVNEYQETINKAKGILMSLACLDR
CCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 3141423 [H]