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The map label for this gene is truA

Identifier: 123966948

GI number: 123966948

Start: 1515389

End: 1516195

Strand: Reverse

Name: truA

Synonym: P9515_17151

Alternate gene names: 123966948

Gene position: 1516195-1515389 (Counterclockwise)

Preceding gene: 123966949

Following gene: 123966947

Centisome position: 88.97

GC content: 32.71

Gene sequence:

>807_bases
TTGAAAAGAGTAGCATTGCTAGTCCAATATGATGGGTCTTATTTTTCTGGTTGGCAAAGACAGAAAAATGCAATAAGTGT
TCAAGAAATGATAGAAGATGCACTTTTCAAAGTGTCTAATCAAATGATTAAAACATTCGCTGCAGGTAGAACGGATTCTG
GGGTTCATGCATCTGGCCAAGTAGTCCATTTTGATATTGATTTTGTAATACCAAGCGATCGATATGCAAATGTATTAAAT
AGTAGATTGCCTTCGACAATTAGAATCTTAGAATCAGTAGAGGTTAAAAAGAATTGGCATGCCTGTTATTCTGCGGTTTA
TAGACATTATCGATATGTTATTAATAATAGTAAGATTCCAAATTTATTTTTAAATAAATGGTCATGGCACAGATACCAAA
AATCTCTAGATGAGTTTTTAATGTCAAAAGCTTTAAATGGAATGATTGGAGAGCACGATTTTTATGCATTTCAAAAGAGT
GGTAGTACTAGATCAACGTCTGTAACAACAATAAAATCAATAGAAATTGAGAGGACGGAGGATTTGATATTAATAGATAT
AAAGGCTACTGGTTTTCTTTATGGGATGGTCCGTTCAATAGTTGGTCAACTTGTTTTAGTAGGAGAAAAGAAGATATCAC
CAGAAATTTTTAAAGATAGATGGGTTGGTAAAAAGAAACAGGATGTTCATGAATCAGCCCCAGCGAACGGCTTATGTTTT
GTTAATTCGGTATATCAAGAAACTATTTTTAAAAGGATTGATAAAAATGATCTTTTCCCGAAATTTGTAATAAGAGGGTA
TTCCTAA

Upstream 100 bases:

>100_bases
AACTGTTGCAAGAAAAGGTGATAACGCTCAAATGGCTATTATTGAACTTGTTTAAAGATAAAAAGATCAATATTTAATTA
ATAAAGATAAATTATAACTT

Downstream 100 bases:

>100_bases
CTTCTATAGTTTTTGTTCTCAAAATTATATGTTTAGATAATCTTAAAATATTGTTGAATACTACTTCAATAATGTAGAAT
TTAAGACTGATTTAAATTTA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRVALLVQYDGSYFSGWQRQKNAISVQEMIEDALFKVSNQMIKTFAAGRTDSGVHASGQVVHFDIDFVIPSDRYANVLN
SRLPSTIRILESVEVKKNWHACYSAVYRHYRYVINNSKIPNLFLNKWSWHRYQKSLDEFLMSKALNGMIGEHDFYAFQKS
GSTRSTSVTTIKSIEIERTEDLILIDIKATGFLYGMVRSIVGQLVLVGEKKISPEIFKDRWVGKKKQDVHESAPANGLCF
VNSVYQETIFKRIDKNDLFPKFVIRGYS

Sequences:

>Translated_268_residues
MKRVALLVQYDGSYFSGWQRQKNAISVQEMIEDALFKVSNQMIKTFAAGRTDSGVHASGQVVHFDIDFVIPSDRYANVLN
SRLPSTIRILESVEVKKNWHACYSAVYRHYRYVINNSKIPNLFLNKWSWHRYQKSLDEFLMSKALNGMIGEHDFYAFQKS
GSTRSTSVTTIKSIEIERTEDLILIDIKATGFLYGMVRSIVGQLVLVGEKKISPEIFKDRWVGKKKQDVHESAPANGLCF
VNSVYQETIFKRIDKNDLFPKFVIRGYS
>Mature_268_residues
MKRVALLVQYDGSYFSGWQRQKNAISVQEMIEDALFKVSNQMIKTFAAGRTDSGVHASGQVVHFDIDFVIPSDRYANVLN
SRLPSTIRILESVEVKKNWHACYSAVYRHYRYVINNSKIPNLFLNKWSWHRYQKSLDEFLMSKALNGMIGEHDFYAFQKS
GSTRSTSVTTIKSIEIERTEDLILIDIKATGFLYGMVRSIVGQLVLVGEKKISPEIFKDRWVGKKKQDVHESAPANGLCF
VNSVYQETIFKRIDKNDLFPKFVIRGYS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=27.7153558052434, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI199559785, Length=256, Percent_Identity=26.953125, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=35.9183673469388, Blast_Score=154, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321112, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007752, Length=310, Percent_Identity=29.3548387096774, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PROM5 (A2BYR1)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012029.1
- ProteinModelPortal:   A2BYR1
- SMR:   A2BYR1
- STRING:   A2BYR1
- GeneID:   4719797
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_17151
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   PMAR167542:P9515ORF_1788-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30842; Mature: 30842

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVALLVQYDGSYFSGWQRQKNAISVQEMIEDALFKVSNQMIKTFAAGRTDSGVHASGQ
CCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VVHFDIDFVIPSDRYANVLNSRLPSTIRILESVEVKKNWHACYSAVYRHYRYVINNSKIP
EEEEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NLFLNKWSWHRYQKSLDEFLMSKALNGMIGEHDFYAFQKSGSTRSTSVTTIKSIEIERTE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCEEEEEEEEEEECCC
DLILIDIKATGFLYGMVRSIVGQLVLVGEKKISPEIFKDRWVGKKKQDVHESAPANGLCF
CEEEEEEEHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCCCCHHHHHHCCCCCCEEE
VNSVYQETIFKRIDKNDLFPKFVIRGYS
HHHHHHHHHHHHCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKRVALLVQYDGSYFSGWQRQKNAISVQEMIEDALFKVSNQMIKTFAAGRTDSGVHASGQ
CCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VVHFDIDFVIPSDRYANVLNSRLPSTIRILESVEVKKNWHACYSAVYRHYRYVINNSKIP
EEEEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NLFLNKWSWHRYQKSLDEFLMSKALNGMIGEHDFYAFQKSGSTRSTSVTTIKSIEIERTE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCEEEEEEEEEEECCC
DLILIDIKATGFLYGMVRSIVGQLVLVGEKKISPEIFKDRWVGKKKQDVHESAPANGLCF
CEEEEEEEHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCCCCHHHHHHCCCCCCEEE
VNSVYQETIFKRIDKNDLFPKFVIRGYS
HHHHHHHHHHHHCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA