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The map label for this gene is lipA1 [H]

Identifier: 123966927

GI number: 123966927

Start: 1493252

End: 1494145

Strand: Reverse

Name: lipA1 [H]

Synonym: P9515_16941

Alternate gene names: 123966927

Gene position: 1494145-1493252 (Counterclockwise)

Preceding gene: 123966930

Following gene: 123966924

Centisome position: 87.68

GC content: 33.56

Gene sequence:

>894_bases
TTGACTAATATTTTAAATAATTCAGTTGCCAAACCTGAATGGTTAAGAGTAAAAGCACCTCAAGTTGAGAGAATTGGTAA
TATTTCAAATTTATTGAGTGATCTAAAATTAAATACAGTTTGTCAAGAAGCAAGCTGTCCTAACATTGGGGAATGTTTTG
CCAGCGGTACTGCAACCTTTTTAATCATGGGCCCTGGTTGCACTAGGGCATGTCCATATTGCGATATTAATTTTGATAGA
TCAAAGAGAGATTTAGACCCAACAGAACCTGACCGTTTAGCAGAGGCTGTACATAGAATGAAACTTAAGCATGTGGTAAT
AACATCAGTTAACAGAGATGATCTTGATGATGGTGGTGCTTCTCAATTCTACAAATGTGTCTCGGAAGTTAGAATAAAGT
CTCCTCAAACTACTATTGAATTATTAATCCCAGATTTATGTGGCAACTGGGGAGCACTTGAACAGGTATTAGATTCTAAA
CCAAATGTTTTGAATCATAATATTGAAACTGTACCAAGCTTGTATAGAAAAGTAAGACCACAAGGAAACTATCAAAGAAC
TTTAGAGTTACTTAAAAGAACAAGAGAATATTTTCCAAATGTTTATACAAAGTCAGGTTTTATGCTTGGATTAGGAGAAA
AGGATGATGAAGTTTTAAATCTCCTAATGGATCTAAGAAGAAATGATGTAGATATAGTTACTATTGGACAATACTTATCT
CCAGGCCCAAAACATCTTCCTGTTCAAAGATTTGTGAGCCCTTCGAAATTTAATTATTTTAAATTATTTGGAGAAGATGA
ATTAGGTTTTATGCAAATCGTGAGTTCTCCTTTAACACGTAGTAGTTATCATGCTGAAGAAATTCAAAAACTCATGAAAA
AACATCCAAGATAA

Upstream 100 bases:

>100_bases
GATTTAAATAGATGTTTAGTTTCTTTCTTGAAGTAAATAAAAATAGTAAAGTTTTACAATTTTGATTAAAATTAAAGTTA
ATTGGGTTGAATAAATTAAT

Downstream 100 bases:

>100_bases
CTAAATTATTAATTGATTGATATCAATCCATTCATTTAGTTTCCATTCAACGATGTTATTTTTTATCATCGGGTCATTTT
TTATAATTTCTAGGGCATCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTNILNNSVAKPEWLRVKAPQVERIGNISNLLSDLKLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDINFDR
SKRDLDPTEPDRLAEAVHRMKLKHVVITSVNRDDLDDGGASQFYKCVSEVRIKSPQTTIELLIPDLCGNWGALEQVLDSK
PNVLNHNIETVPSLYRKVRPQGNYQRTLELLKRTREYFPNVYTKSGFMLGLGEKDDEVLNLLMDLRRNDVDIVTIGQYLS
PGPKHLPVQRFVSPSKFNYFKLFGEDELGFMQIVSSPLTRSSYHAEEIQKLMKKHPR

Sequences:

>Translated_297_residues
MTNILNNSVAKPEWLRVKAPQVERIGNISNLLSDLKLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDINFDR
SKRDLDPTEPDRLAEAVHRMKLKHVVITSVNRDDLDDGGASQFYKCVSEVRIKSPQTTIELLIPDLCGNWGALEQVLDSK
PNVLNHNIETVPSLYRKVRPQGNYQRTLELLKRTREYFPNVYTKSGFMLGLGEKDDEVLNLLMDLRRNDVDIVTIGQYLS
PGPKHLPVQRFVSPSKFNYFKLFGEDELGFMQIVSSPLTRSSYHAEEIQKLMKKHPR
>Mature_296_residues
TNILNNSVAKPEWLRVKAPQVERIGNISNLLSDLKLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDINFDRS
KRDLDPTEPDRLAEAVHRMKLKHVVITSVNRDDLDDGGASQFYKCVSEVRIKSPQTTIELLIPDLCGNWGALEQVLDSKP
NVLNHNIETVPSLYRKVRPQGNYQRTLELLKRTREYFPNVYTKSGFMLGLGEKDDEVLNLLMDLRRNDVDIVTIGQYLSP
GPKHLPVQRFVSPSKFNYFKLFGEDELGFMQIVSSPLTRSSYHAEEIQKLMKKHPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=43.1095406360424, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=42.798353909465, Blast_Score=207, Evalue=6e-54,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=47.3684210526316, Blast_Score=275, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=43.359375, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=292, Percent_Identity=39.7260273972603, Blast_Score=226, Evalue=3e-60,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=42.756183745583, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33675; Mature: 33544

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNILNNSVAKPEWLRVKAPQVERIGNISNLLSDLKLNTVCQEASCPNIGECFASGTATF
CCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEE
LIMGPGCTRACPYCDINFDRSKRDLDPTEPDRLAEAVHRMKLKHVVITSVNRDDLDDGGA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCH
SQFYKCVSEVRIKSPQTTIELLIPDLCGNWGALEQVLDSKPNVLNHNIETVPSLYRKVRP
HHHHHHHHHHHCCCCCHHHHHEEHHHCCCHHHHHHHHCCCCCHHCCCHHHHHHHHHHHCC
QGNYQRTLELLKRTREYFPNVYTKSGFMLGLGEKDDEVLNLLMDLRRNDVDIVTIGQYLS
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECHHHC
PGPKHLPVQRFVSPSKFNYFKLFGEDELGFMQIVSSPLTRSSYHAEEIQKLMKKHPR
CCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TNILNNSVAKPEWLRVKAPQVERIGNISNLLSDLKLNTVCQEASCPNIGECFASGTATF
CCCCCCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEE
LIMGPGCTRACPYCDINFDRSKRDLDPTEPDRLAEAVHRMKLKHVVITSVNRDDLDDGGA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCH
SQFYKCVSEVRIKSPQTTIELLIPDLCGNWGALEQVLDSKPNVLNHNIETVPSLYRKVRP
HHHHHHHHHHHCCCCCHHHHHEEHHHCCCHHHHHHHHCCCCCHHCCCHHHHHHHHHHHCC
QGNYQRTLELLKRTREYFPNVYTKSGFMLGLGEKDDEVLNLLMDLRRNDVDIVTIGQYLS
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECHHHC
PGPKHLPVQRFVSPSKFNYFKLFGEDELGFMQIVSSPLTRSSYHAEEIQKLMKKHPR
CCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]