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The map label for this gene is queF

Identifier: 123966873

GI number: 123966873

Start: 1432805

End: 1433215

Strand: Reverse

Name: queF

Synonym: P9515_16401

Alternate gene names: 123966873

Gene position: 1433215-1432805 (Counterclockwise)

Preceding gene: 123966876

Following gene: 123966869

Centisome position: 84.1

GC content: 27.74

Gene sequence:

>411_bases
ATGAGTACACCTAACTTATACGATTCAACTAATAAGCCTTTATATGGAGAAAGACTGATAGAAGAATCAAAAATAATTTG
CTTTGATAATCCAAACAAAAAAAGAATTTATGAAATTTCAATTGAACTTCCTGAATTTACTTGCAAATGCCCATTTTCTG
GTTATCCAGATTTTGCAAAATTAAATATTTTTTATCAGCCTAATAGTAAAGTTTATGAGTTGAAATCTTTAAAACTTTAT
ATTAATCACTTTAGAGATCTTAAGATATCACATGAAGAAGTAGTAAACAGAATTATGGATGATCTTTTAAATGCAGCAGC
TCCTCATTGGATACATTTAAATGCTGATTTTAATCCTAGAGGAAACGTTTCTATGAAATTAGATATTTACAGTGGGCAGA
AAAGAAACTAA

Upstream 100 bases:

>100_bases
ATCCTCTGACTTATTGATTTGTAACACTAAGATATGTTTTTGATTTTTTGCGAATTCTTAGCTTTTTATATATTTTTAGT
AGAGAAGATTGTTAAAAACA

Downstream 100 bases:

>100_bases
TTATTCTTTATCTCATCTGATAATTTTTGAAATTCCTTCTTGAAGCCTAAAAGATTTCTATTACTTAAACCACCAACAAA
TAATTTATTTGAATTTTCAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSTPNLYDSTNKPLYGERLIEESKIICFDNPNKKRIYEISIELPEFTCKCPFSGYPDFAKLNIFYQPNSKVYELKSLKLY
INHFRDLKISHEEVVNRIMDDLLNAAAPHWIHLNADFNPRGNVSMKLDIYSGQKRN

Sequences:

>Translated_136_residues
MSTPNLYDSTNKPLYGERLIEESKIICFDNPNKKRIYEISIELPEFTCKCPFSGYPDFAKLNIFYQPNSKVYELKSLKLY
INHFRDLKISHEEVVNRIMDDLLNAAAPHWIHLNADFNPRGNVSMKLDIYSGQKRN
>Mature_135_residues
STPNLYDSTNKPLYGERLIEESKIICFDNPNKKRIYEISIELPEFTCKCPFSGYPDFAKLNIFYQPNSKVYELKSLKLYI
NHFRDLKISHEEVVNRIMDDLLNAAAPHWIHLNADFNPRGNVSMKLDIYSGQKRN

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_PROM5 (A2BYI6)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011954.1
- ProteinModelPortal:   A2BYI6
- SMR:   A2BYI6
- STRING:   A2BYI6
- GeneID:   4720514
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_16401
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- BioCyc:   PMAR167542:P9515ORF_1710-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 15946; Mature: 15815

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPNLYDSTNKPLYGERLIEESKIICFDNPNKKRIYEISIELPEFTCKCPFSGYPDFAK
CCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCEEEEEEEECCCEEEECCCCCCCCCEE
LNIFYQPNSKVYELKSLKLYINHFRDLKISHEEVVNRIMDDLLNAAAPHWIHLNADFNPR
EEEEECCCCCEEEEEHHEEEEEHHCCCEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
GNVSMKLDIYSGQKRN
CCEEEEEEECCCCCCC
>Mature Secondary Structure 
STPNLYDSTNKPLYGERLIEESKIICFDNPNKKRIYEISIELPEFTCKCPFSGYPDFAK
CCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCEEEEEEEECCCEEEECCCCCCCCCEE
LNIFYQPNSKVYELKSLKLYINHFRDLKISHEEVVNRIMDDLLNAAAPHWIHLNADFNPR
EEEEECCCCCEEEEEHHEEEEEHHCCCEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
GNVSMKLDIYSGQKRN
CCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA