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The map label for this gene is cbiB [H]

Identifier: 123966710

GI number: 123966710

Start: 1314301

End: 1315314

Strand: Direct

Name: cbiB [H]

Synonym: P9515_14771

Alternate gene names: 123966710

Gene position: 1314301-1315314 (Clockwise)

Preceding gene: 123966709

Following gene: 123966711

Centisome position: 77.12

GC content: 27.51

Gene sequence:

>1014_bases
TTGGCTGAAATAAATTTATTTTATATATTTATTGGATCGATTGGTTTTGATCTTTTAATTGGTGATCCAAGATTTATCAT
CCATCCTGTTCAAATAATAGGGTTCTATATTAAAAAAATAACTAATTTTTTTATTCATCATTTCAAGGGAAACAAGAGAA
TACTATTTTGGGGAGGTTTATTTATTGCATTATCTTCCATTGGAATAAGCTTTGAGATCGGGAAATTAATTGAATTAGTA
TTTCTGCAATCAAAAAGTAATTTAATTTCTGGAATATTAATTTTCTTTGGTTTATCAAGTTGTTTGGCATCAAAAAGTCT
TATTTCAAGCGTAAAAGAAATTTCTGACCTAATTAATAGCAGTGGGACTGATTCAAAAACTGAAAAATTAGTTAGAGAAA
AAGTTCAAAGAATTGTAAGTAGAGATGTAAGCACATCTTCGTTAGAGCATCTCATGAGATCAAGTAGTGAGAGTCTTACT
GAAAATTCAGTGGATGGGATTTTTGGACCACTTTTTTGGATATTTATAGGATTAGTTTTTATGAAATTTTCAATTTTTCT
CCCAGGACCTTTATCTCTTGGCTTTTCATATAAAACTATAAGTACTTTAGATTCAATGATCGGCTATAAATATGATCACT
ATAAATTTCTAGGTTTTTTTAGTGCAAAAATTGAAGATTATTCAACTTTCCTTCCAAGTAGATTAGTCTTATTAACATTG
CCTTTGGTTAGCTTTAAGATAAATCAATATATTTCTATCGTTAAAAAAAGTTACTTTGAAGGCAGTAAATACGATTCCCC
AAACTCTGGAATTTCTGAAGCGATTTTTGCTTATACTTCCAATATAAAGTTAGGGGGCAAAAGTAAATATAATAATCAAA
TTGTTGAAAAACCTGTTATTAATTCACATGGTGATATTTGCACAAAAGAAAAAATAAATCTTATTTGTCAACTAATTTAC
AGACTTCAATTATTGTGGATATTATTTTTTACGCTAATTTTCTTCAATATTTAA

Upstream 100 bases:

>100_bases
AAGAGTGTGATAGGGATAAACCATTAATGACTAAATTAAGAGCAGAAAATTCGCAACATGAGATAGAAAAAGTTGGGAAA
GGTCTTCGTGCAATGTTCAG

Downstream 100 bases:

>100_bases
GTTTATATTCAATAAAATTTGTTTAAAATAGCTATTACTAATATAAACATTTTTCATGAAAGAAGAAATTCCTAAAATAG
AAAATAAAAATGAGAATTCA

Product: putative cobalamin biosynthetic protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAEINLFYIFIGSIGFDLLIGDPRFIIHPVQIIGFYIKKITNFFIHHFKGNKRILFWGGLFIALSSIGISFEIGKLIELV
FLQSKSNLISGILIFFGLSSCLASKSLISSVKEISDLINSSGTDSKTEKLVREKVQRIVSRDVSTSSLEHLMRSSSESLT
ENSVDGIFGPLFWIFIGLVFMKFSIFLPGPLSLGFSYKTISTLDSMIGYKYDHYKFLGFFSAKIEDYSTFLPSRLVLLTL
PLVSFKINQYISIVKKSYFEGSKYDSPNSGISEAIFAYTSNIKLGGKSKYNNQIVEKPVINSHGDICTKEKINLICQLIY
RLQLLWILFFTLIFFNI

Sequences:

>Translated_337_residues
MAEINLFYIFIGSIGFDLLIGDPRFIIHPVQIIGFYIKKITNFFIHHFKGNKRILFWGGLFIALSSIGISFEIGKLIELV
FLQSKSNLISGILIFFGLSSCLASKSLISSVKEISDLINSSGTDSKTEKLVREKVQRIVSRDVSTSSLEHLMRSSSESLT
ENSVDGIFGPLFWIFIGLVFMKFSIFLPGPLSLGFSYKTISTLDSMIGYKYDHYKFLGFFSAKIEDYSTFLPSRLVLLTL
PLVSFKINQYISIVKKSYFEGSKYDSPNSGISEAIFAYTSNIKLGGKSKYNNQIVEKPVINSHGDICTKEKINLICQLIY
RLQLLWILFFTLIFFNI
>Mature_336_residues
AEINLFYIFIGSIGFDLLIGDPRFIIHPVQIIGFYIKKITNFFIHHFKGNKRILFWGGLFIALSSIGISFEIGKLIELVF
LQSKSNLISGILIFFGLSSCLASKSLISSVKEISDLINSSGTDSKTEKLVREKVQRIVSRDVSTSSLEHLMRSSSESLTE
NSVDGIFGPLFWIFIGLVFMKFSIFLPGPLSLGFSYKTISTLDSMIGYKYDHYKFLGFFSAKIEDYSTFLPSRLVLLTLP
LVSFKINQYISIVKKSYFEGSKYDSPNSGISEAIFAYTSNIKLGGKSKYNNQIVEKPVINSHGDICTKEKINLICQLIYR
LQLLWILFFTLIFFNI

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 38162; Mature: 38031

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEINLFYIFIGSIGFDLLIGDPRFIIHPVQIIGFYIKKITNFFIHHFKGNKRILFWGGL
CCCEEEEEEEEECCCCEEEECCCHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHH
FIALSSIGISFEIGKLIELVFLQSKSNLISGILIFFGLSSCLASKSLISSVKEISDLINS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGTDSKTEKLVREKVQRIVSRDVSTSSLEHLMRSSSESLTENSVDGIFGPLFWIFIGLVF
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHH
MKFSIFLPGPLSLGFSYKTISTLDSMIGYKYDHYKFLGFFSAKIEDYSTFLPSRLVLLTL
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVSFKINQYISIVKKSYFEGSKYDSPNSGISEAIFAYTSNIKLGGKSKYNNQIVEKPVI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCHHHCCCCC
NSHGDICTKEKINLICQLIYRLQLLWILFFTLIFFNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEINLFYIFIGSIGFDLLIGDPRFIIHPVQIIGFYIKKITNFFIHHFKGNKRILFWGGL
CCEEEEEEEEECCCCEEEECCCHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHH
FIALSSIGISFEIGKLIELVFLQSKSNLISGILIFFGLSSCLASKSLISSVKEISDLINS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGTDSKTEKLVREKVQRIVSRDVSTSSLEHLMRSSSESLTENSVDGIFGPLFWIFIGLVF
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHH
MKFSIFLPGPLSLGFSYKTISTLDSMIGYKYDHYKFLGFFSAKIEDYSTFLPSRLVLLTL
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVSFKINQYISIVKKSYFEGSKYDSPNSGISEAIFAYTSNIKLGGKSKYNNQIVEKPVI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCHHHCCCCC
NSHGDICTKEKINLICQLIYRLQLLWILFFTLIFFNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]