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The map label for this gene is panB

Identifier: 123966704

GI number: 123966704

Start: 1308670

End: 1309443

Strand: Direct

Name: panB

Synonym: P9515_14711

Alternate gene names: 123966704

Gene position: 1308670-1309443 (Clockwise)

Preceding gene: 123966703

Following gene: 123966706

Centisome position: 76.79

GC content: 35.92

Gene sequence:

>774_bases
ATGTTGCCATCAGAACTCGTTAAATATAAAAAAAATTCTCAAAAAATCATTGCATTAACTGCTTGGGACTCCATATCAGG
TTCTCTAGCTGAGCAATCAGGAGCAGATATTGTTCTAGTAGGAGACTCTTTAGCAATGGTGTGTTTAGGATACAAATCCA
CATTACCAGTAACTTTAGAGAATATGATTTATCACACAAATGCAGTTTCAAGAGGGTTCAGCAGAGAAATAGAAGAACAT
TCCTTATTAGTTTGTGATATGCCTTTTCTAACTTATCAATGCGGAGAAGATAAGGCAGTGGAATATGCCGGGAAAATAAT
TAAGAACACCTATGCAAAGGCAGTAAAAATTGAAGGTGCTGAACCAGAGGTCCAAACTGTTATTTCAAGGCTAATAAGAA
TGGGGATTCCTGTCATGGGGCATTTAGGACTTACACCACAGAGTTATTTAAATCTAGGATTAAAAAAACAAGGGCTCAAG
AAAGAAAGTTACGAAAAAATCAAAAGAGATGCTTTATCACTAGAAAAATTGGGATGCTTTTCTATAGTTTTGGAGCATAT
TCCGGAATTACTTGCTAAAGAAATTCAAACCGATTTAGAAATACCGACAATAGGAATAGGTGCAGGCGTCTTTTGTGATG
GTCAGGTAAGAGTTACGGCTGATTTATTAGGCCTAAGTGATAAGCAGCCTCCATTTTGTAAACCAATTGTTGATGGGAAA
AAATATTTTGGTGAAAAACTTAAAGAGTGGGTAACTTCTGAAAGGCTTAGTTAA

Upstream 100 bases:

>100_bases
TGCTACCTTCCGATCCTGACCAGGTTTGGGTGTTAAAACCGCGAAAGGCCGAGTCAAAATTATACTAGCAATTTTTGATA
AAAAAAGATACATAACTCTT

Downstream 100 bases:

>100_bases
CTTTGTCCCACCATCTAAACATTGAGACAAGAATTTGATTGCTTAATTCCATACCTTTTGGATCACTCAAAAAAAATCTA
TTACCTCTTTTAATTAGAAG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLPSELVKYKKNSQKIIALTAWDSISGSLAEQSGADIVLVGDSLAMVCLGYKSTLPVTLENMIYHTNAVSRGFSREIEEH
SLLVCDMPFLTYQCGEDKAVEYAGKIIKNTYAKAVKIEGAEPEVQTVISRLIRMGIPVMGHLGLTPQSYLNLGLKKQGLK
KESYEKIKRDALSLEKLGCFSIVLEHIPELLAKEIQTDLEIPTIGIGAGVFCDGQVRVTADLLGLSDKQPPFCKPIVDGK
KYFGEKLKEWVTSERLS

Sequences:

>Translated_257_residues
MLPSELVKYKKNSQKIIALTAWDSISGSLAEQSGADIVLVGDSLAMVCLGYKSTLPVTLENMIYHTNAVSRGFSREIEEH
SLLVCDMPFLTYQCGEDKAVEYAGKIIKNTYAKAVKIEGAEPEVQTVISRLIRMGIPVMGHLGLTPQSYLNLGLKKQGLK
KESYEKIKRDALSLEKLGCFSIVLEHIPELLAKEIQTDLEIPTIGIGAGVFCDGQVRVTADLLGLSDKQPPFCKPIVDGK
KYFGEKLKEWVTSERLS
>Mature_257_residues
MLPSELVKYKKNSQKIIALTAWDSISGSLAEQSGADIVLVGDSLAMVCLGYKSTLPVTLENMIYHTNAVSRGFSREIEEH
SLLVCDMPFLTYQCGEDKAVEYAGKIIKNTYAKAVKIEGAEPEVQTVISRLIRMGIPVMGHLGLTPQSYLNLGLKKQGLK
KESYEKIKRDALSLEKLGCFSIVLEHIPELLAKEIQTDLEIPTIGIGAGVFCDGQVRVTADLLGLSDKQPPFCKPIVDGK
KYFGEKLKEWVTSERLS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=234, Percent_Identity=43.1623931623932, Blast_Score=157, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6319653, Length=246, Percent_Identity=39.4308943089431, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PROM5 (A2BY17)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011785.1
- STRING:   A2BY17
- GeneID:   4720119
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_14711
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   PMAR167542:P9515ORF_1539-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28323; Mature: 28323

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: ACT_SITE 185-185 BINDING 86-86 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSELVKYKKNSQKIIALTAWDSISGSLAEQSGADIVLVGDSLAMVCLGYKSTLPVTLE
CCHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCCEEEECCCCEEHHHCCCCCCCEEHH
NMIYHTNAVSRGFSREIEEHSLLVCDMPFLTYQCGEDKAVEYAGKIIKNTYAKAVKIEGA
HHHHHHHHHHHHHHHHHHHCCEEEEECCHHEEECCCCHHHHHHHHHHHHHHHHEEEECCC
EPEVQTVISRLIRMGIPVMGHLGLTPQSYLNLGLKKQGLKKESYEKIKRDALSLEKLGCF
CCHHHHHHHHHHHHCCCCEECCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
SIVLEHIPELLAKEIQTDLEIPTIGIGAGVFCDGQVRVTADLLGLSDKQPPFCKPIVDGK
HHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCHH
KYFGEKLKEWVTSERLS
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPSELVKYKKNSQKIIALTAWDSISGSLAEQSGADIVLVGDSLAMVCLGYKSTLPVTLE
CCHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCCEEEECCCCEEHHHCCCCCCCEEHH
NMIYHTNAVSRGFSREIEEHSLLVCDMPFLTYQCGEDKAVEYAGKIIKNTYAKAVKIEGA
HHHHHHHHHHHHHHHHHHHCCEEEEECCHHEEECCCCHHHHHHHHHHHHHHHHEEEECCC
EPEVQTVISRLIRMGIPVMGHLGLTPQSYLNLGLKKQGLKKESYEKIKRDALSLEKLGCF
CCHHHHHHHHHHHHCCCCEECCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
SIVLEHIPELLAKEIQTDLEIPTIGIGAGVFCDGQVRVTADLLGLSDKQPPFCKPIVDGK
HHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCHH
KYFGEKLKEWVTSERLS
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA