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The map label for this gene is rfbA [H]

Identifier: 123966643

GI number: 123966643

Start: 1249306

End: 1250196

Strand: Reverse

Name: rfbA [H]

Synonym: P9515_14101

Alternate gene names: 123966643

Gene position: 1250196-1249306 (Counterclockwise)

Preceding gene: 123966647

Following gene: 123966633

Centisome position: 73.36

GC content: 32.66

Gene sequence:

>891_bases
ATGATTAATTTAGAAATGAAAAAAAGAAAAGGGATAATTCTTGCTGGAGGTTTAGGTACCAGATTAAGTCCAATATCTTT
AGCTATAAGTAAGCAACTCATGCCGGTATACGATAAGCCTATGATTTATTATCCATTAACCACACTTATGCTGGCTGATA
TAAATGATATTTTATTAATCTCATCTCCTGATCATTTAAATTCATTTAAGAGATTGTTAGGAGATGGAAAATCTCTTGGC
ATTAATATTGAATATGCTTCTCAGGAAAAACCACAGGGAATTGCTCAAGCTTTAATTATTGGGGAAAAGTTTCTAAATGG
ATCCCCATCAGTTTTAATACTTGGTGATAATTTATTCCATGGGAATGAACTTCTACCTAAACTAGAATCTGCAAATCTCA
AAAAAGATGGAGCAACAATATTCGCATACCCTGTAAGTGACCCGGAAAGATATGGAGTTGTTTATTTCAATAAATCAGGC
AAGGCCTGTTCTATTGAAGAAAAACCTTCAAATCCAAAAAGTAGATTTGCAATAACAGGAATTTATTTCTATGATGATAC
CGTCATCTCTAAAGCAAAAAAGATAAAACCATCTCTTAGAGGAGAATTAGAGATATCTTCAATTAACAAGTTGTTTCTGC
AAGAATCAAATTTAAATGTTGAAATAATGGGTAGAGGTATGGCATGGCTAGATACTGGAACTTTTGATTCTTTAAATGAA
GCGAGTAACTATATTAGGGCTTTGGAAAAAAGACAGGGTTTAAAAATCGGATCCCCAGAAGAAGTAGCCTGGCGTAAAGG
TTGGATAAAAGATTCTCAACTTAAAGAACTTGGAAATGCTCTTAAGTCAAGTTCTTATGGTAAGTATCTTTTAGAACTAT
TAAAAAATTAG

Upstream 100 bases:

>100_bases
TCTGAGATCTTCAAAGTTGATAAATAAAAAAAAATAAATTTAGTTATATTTTAACCTTTTTTTAGTTAGGAAACATACTA
ATCTTAGATATATATAACTA

Downstream 100 bases:

>100_bases
GAGTTTTGATATTCAAAAATAAAAAAATTTCTTATAAATATTAAAAGTAAATATTTTTTTAATTAATCTAAATAAAGTTA
AAAATAAGAGAGGATCAACT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MINLEMKKRKGIILAGGLGTRLSPISLAISKQLMPVYDKPMIYYPLTTLMLADINDILLISSPDHLNSFKRLLGDGKSLG
INIEYASQEKPQGIAQALIIGEKFLNGSPSVLILGDNLFHGNELLPKLESANLKKDGATIFAYPVSDPERYGVVYFNKSG
KACSIEEKPSNPKSRFAITGIYFYDDTVISKAKKIKPSLRGELEISSINKLFLQESNLNVEIMGRGMAWLDTGTFDSLNE
ASNYIRALEKRQGLKIGSPEEVAWRKGWIKDSQLKELGNALKSSSYGKYLLELLKN

Sequences:

>Translated_296_residues
MINLEMKKRKGIILAGGLGTRLSPISLAISKQLMPVYDKPMIYYPLTTLMLADINDILLISSPDHLNSFKRLLGDGKSLG
INIEYASQEKPQGIAQALIIGEKFLNGSPSVLILGDNLFHGNELLPKLESANLKKDGATIFAYPVSDPERYGVVYFNKSG
KACSIEEKPSNPKSRFAITGIYFYDDTVISKAKKIKPSLRGELEISSINKLFLQESNLNVEIMGRGMAWLDTGTFDSLNE
ASNYIRALEKRQGLKIGSPEEVAWRKGWIKDSQLKELGNALKSSSYGKYLLELLKN
>Mature_296_residues
MINLEMKKRKGIILAGGLGTRLSPISLAISKQLMPVYDKPMIYYPLTTLMLADINDILLISSPDHLNSFKRLLGDGKSLG
INIEYASQEKPQGIAQALIIGEKFLNGSPSVLILGDNLFHGNELLPKLESANLKKDGATIFAYPVSDPERYGVVYFNKSG
KACSIEEKPSNPKSRFAITGIYFYDDTVISKAKKIKPSLRGELEISSINKLFLQESNLNVEIMGRGMAWLDTGTFDSLNE
ASNYIRALEKRQGLKIGSPEEVAWRKGWIKDSQLKELGNALKSSSYGKYLLELLKN

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=253, Percent_Identity=23.7154150197628, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI11761619, Length=253, Percent_Identity=23.7154150197628, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=58.3916083916084, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788351, Length=291, Percent_Identity=57.3883161512027, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787488, Length=257, Percent_Identity=23.7354085603113, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133931050, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=257, Percent_Identity=27.6264591439689, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355443, Length=254, Percent_Identity=27.5590551181102, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644084, Length=254, Percent_Identity=27.5590551181102, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32837; Mature: 32837

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLEMKKRKGIILAGGLGTRLSPISLAISKQLMPVYDKPMIYYPLTTLMLADINDILLI
CCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCEEEE
SSPDHLNSFKRLLGDGKSLGINIEYASQEKPQGIAQALIIGEKFLNGSPSVLILGDNLFH
ECCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEC
GNELLPKLESANLKKDGATIFAYPVSDPERYGVVYFNKSGKACSIEEKPSNPKSRFAITG
CCHHCCCHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCCCCCCCEEEEEE
IYFYDDTVISKAKKIKPSLRGELEISSINKLFLQESNLNVEIMGRGMAWLDTGTFDSLNE
EEEECCHHHHHHHHCCCCCCCEEEHHHHHEEEEECCCCEEEEEECCEEEEECCCCCCHHH
ASNYIRALEKRQGLKIGSPEEVAWRKGWIKDSQLKELGNALKSSSYGKYLLELLKN
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MINLEMKKRKGIILAGGLGTRLSPISLAISKQLMPVYDKPMIYYPLTTLMLADINDILLI
CCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCEEEE
SSPDHLNSFKRLLGDGKSLGINIEYASQEKPQGIAQALIIGEKFLNGSPSVLILGDNLFH
ECCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCEEC
GNELLPKLESANLKKDGATIFAYPVSDPERYGVVYFNKSGKACSIEEKPSNPKSRFAITG
CCHHCCCHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCCCCCCCEEEEEE
IYFYDDTVISKAKKIKPSLRGELEISSINKLFLQESNLNVEIMGRGMAWLDTGTFDSLNE
EEEECCHHHHHHHHCCCCCCCEEEHHHHHEEEEECCCCEEEEEECCEEEEECCCCCCHHH
ASNYIRALEKRQGLKIGSPEEVAWRKGWIKDSQLKELGNALKSSSYGKYLLELLKN
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]