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The map label for this gene is pntA [H]

Identifier: 123966545

GI number: 123966545

Start: 1153765

End: 1154895

Strand: Reverse

Name: pntA [H]

Synonym: P9515_13121

Alternate gene names: 123966545

Gene position: 1154895-1153765 (Counterclockwise)

Preceding gene: 123966549

Following gene: 123966544

Centisome position: 67.77

GC content: 37.4

Gene sequence:

>1131_bases
TTGACAAAGATACTCATTCCTATCGAAACAGTCTCTGGAGAGAGAAGAGTTTCAGCCACTCCAGGAGCTGTGAAAAAATT
AAAAAGTCTTGGATGCGAAGTTTTTGTAGAAAGTTCAGCAGGAGAACTATCAGGGTTTAATGACACTTTATACAAAGAAT
CTGGAGGTCAAATTGTTACTGAATCTAATGCAAATATTTGGGAGAATACCGATGTTGTTTTTTGTGTTCAAACGCCTTCT
GAATCTAATTTAGGCAAGTTAAAAAGAGGTGCTATTCTGCTAGGCCTTTTAAATCCATATTCCAATAAACAACTTCAAAA
AACTATAACTAGTAAAAAGATCTCGGCTTTATCAATGGAATTACTCCCAAGGATTAGTAGAGCTCAATCCTCTGATGTAC
TTTCTTCTCAAGCCAATATCGCAGGATATAAAGCAGTTCTTTTGGCAGCAAGTGAACTTGATAGATATTTTCCAATGCTG
ATGACTGCAGCCGGAACCATACAGCCTGCGAAAGTTGTTGTTCTTGGTGGAGGGGTTGCAGGATTGCAGGCAGTTGCTAC
TGCAAAAAGACTTGGAGCTATTGTGTTCGTGTCGGATATTAGACCAGCTGTAAAAGAACAAGTTGAGTCTCTAGGAGCAA
GATTTATAGAACTTCCTGAAATCGACGAAAAACCAGGGGAATCAGGTGGTTATGCAAAAGCTGTTACGCCTGAATTCCTT
TCTAAACAAAAGGCTACCTTAACTAAATATTTATCTGAAGCTGATGTTGCTGTTTGTACGGCACAAGTCTTAGGTAAAAA
AGCTCCAGTTTTAATTGATTCTCATATGATTGCAAATATGCGATCTGGTGCAGTAGTAATTGATCTAGCCGTATCCCAAG
GAGGTAATTGTGAAGGAACAAAATCAAATGAAACTATTATTAGGAATGGTGTAAAGCTTATAGGGGCTGGTGAATTGCCC
TCATCTGTTCCATATGATGCAAGTACACTTTATGCTAAGAACCTAACATCCTTGATTACACCTTTCATAAAAGATGGTGT
AATTAATTTAGACAAAGATGATGAATTAATCTCTGGCTGTTTATTAAGTGATGAAGGAGTGATCCTTCAAAACAAAGTTT
TTGAAAATTGA

Upstream 100 bases:

>100_bases
TGATTTAAAAAAAAGAAAATATTTTTTTAACAAAACTCAAACTCCAAGACTTTTGACTTTATTCAAATTATCTTAAGTAT
GTTTAAAAAAATAATAGAAA

Downstream 100 bases:

>100_bases
GGTTTTAATTGAATTATGAATTTTGATAGCTTGCTCTGGGTTTTATTGCTTGGAAGTTTATTAGGTTTGGAGTTGATAGG
AAAGGTACCTCCAACTCTTC

Product: putative nicotinamide nucleotide transhydrogenase, subunit alpha 1 (A1)

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTKILIPIETVSGERRVSATPGAVKKLKSLGCEVFVESSAGELSGFNDTLYKESGGQIVTESNANIWENTDVVFCVQTPS
ESNLGKLKRGAILLGLLNPYSNKQLQKTITSKKISALSMELLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPML
MTAAGTIQPAKVVVLGGGVAGLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEIDEKPGESGGYAKAVTPEFL
SKQKATLTKYLSEADVAVCTAQVLGKKAPVLIDSHMIANMRSGAVVIDLAVSQGGNCEGTKSNETIIRNGVKLIGAGELP
SSVPYDASTLYAKNLTSLITPFIKDGVINLDKDDELISGCLLSDEGVILQNKVFEN

Sequences:

>Translated_376_residues
MTKILIPIETVSGERRVSATPGAVKKLKSLGCEVFVESSAGELSGFNDTLYKESGGQIVTESNANIWENTDVVFCVQTPS
ESNLGKLKRGAILLGLLNPYSNKQLQKTITSKKISALSMELLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPML
MTAAGTIQPAKVVVLGGGVAGLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEIDEKPGESGGYAKAVTPEFL
SKQKATLTKYLSEADVAVCTAQVLGKKAPVLIDSHMIANMRSGAVVIDLAVSQGGNCEGTKSNETIIRNGVKLIGAGELP
SSVPYDASTLYAKNLTSLITPFIKDGVINLDKDDELISGCLLSDEGVILQNKVFEN
>Mature_375_residues
TKILIPIETVSGERRVSATPGAVKKLKSLGCEVFVESSAGELSGFNDTLYKESGGQIVTESNANIWENTDVVFCVQTPSE
SNLGKLKRGAILLGLLNPYSNKQLQKTITSKKISALSMELLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPMLM
TAAGTIQPAKVVVLGGGVAGLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEIDEKPGESGGYAKAVTPEFLS
KQKATLTKYLSEADVAVCTAQVLGKKAPVLIDSHMIANMRSGAVVIDLAVSQGGNCEGTKSNETIIRNGVKLIGAGELPS
SVPYDASTLYAKNLTSLITPFIKDGVINLDKDDELISGCLLSDEGVILQNKVFEN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=357, Percent_Identity=40.6162464985994, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI122939153, Length=357, Percent_Identity=40.6162464985994, Blast_Score=242, Evalue=4e-64,
Organism=Escherichia coli, GI1787887, Length=369, Percent_Identity=42.2764227642276, Blast_Score=279, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17550456, Length=364, Percent_Identity=40.3846153846154, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39821; Mature: 39690

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKILIPIETVSGERRVSATPGAVKKLKSLGCEVFVESSAGELSGFNDTLYKESGGQIVT
CCEEEEEEEECCCCCEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHEECCCCEEEE
ESNANIWENTDVVFCVQTPSESNLGKLKRGAILLGLLNPYSNKQLQKTITSKKISALSME
CCCCCEECCCCEEEEEECCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPMLMTAAGTIQPAKVVVLGGGVA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
GLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEIDEKPGESGGYAKAVTPEFL
HHHHHHHHHHHCEEEEEHHCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCEECCCCHHHH
SKQKATLTKYLSEADVAVCTAQVLGKKAPVLIDSHMIANMRSGAVVIDLAVSQGGNCEGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCEEEEEEEECCCCCCCCC
KSNETIIRNGVKLIGAGELPSSVPYDASTLYAKNLTSLITPFIKDGVINLDKDDELISGC
CCCCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHE
LLSDEGVILQNKVFEN
EECCCCEEECCCCCCC
>Mature Secondary Structure 
TKILIPIETVSGERRVSATPGAVKKLKSLGCEVFVESSAGELSGFNDTLYKESGGQIVT
CEEEEEEEECCCCCEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCCHHEECCCCEEEE
ESNANIWENTDVVFCVQTPSESNLGKLKRGAILLGLLNPYSNKQLQKTITSKKISALSME
CCCCCEECCCCEEEEEECCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPMLMTAAGTIQPAKVVVLGGGVA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
GLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEIDEKPGESGGYAKAVTPEFL
HHHHHHHHHHHCEEEEEHHCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCEECCCCHHHH
SKQKATLTKYLSEADVAVCTAQVLGKKAPVLIDSHMIANMRSGAVVIDLAVSQGGNCEGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCEEEEEEEECCCCCCCCC
KSNETIIRNGVKLIGAGELPSSVPYDASTLYAKNLTSLITPFIKDGVINLDKDDELISGC
CCCCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHE
LLSDEGVILQNKVFEN
EECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]