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The map label for this gene is pntB [H]

Identifier: 123966543

GI number: 123966543

Start: 1152010

End: 1153446

Strand: Reverse

Name: pntB [H]

Synonym: P9515_13101

Alternate gene names: 123966543

Gene position: 1153446-1152010 (Counterclockwise)

Preceding gene: 123966544

Following gene: 123966542

Centisome position: 67.68

GC content: 37.37

Gene sequence:

>1437_bases
ATGAATCTTCCTGATATTATTAAATTCGTCATAGATCTTCTAGCTGTTCTTTTACTAGCACTTGGAATAAAGGGGTTATC
AAAAGTCAGGTCTGCAAGAGAAGCCAACATATTAGCAGCATTTGCTATGTTTCTTTCAGTTGTAGGTTTGCTCTCTTACT
ACTTAGGTACTTCTGGAATCCCCGCTCAATCATGGATATGGATCATTGTTGGATCTCTAGTAGGTAGTTTATTTGGAACT
TTGCTTGCTAAGAAGGTTCCAATGACATCAATGCCAGAGACTGTAGCTCTGTTCAATGGTTTTGGTGGAATGTCTTCTCT
ATTAGTAGCTATTGGTGCCTTTTTATTCCCTTTTAATGAATTAGCAAAAGCAGGATCTTTAGAGCCTTTTATTAATAATG
TTTCAATTTCAGTTTCAATATTTGTTGGAGCCATTACATTTTCAGGCTCAATTGTGGCTATGGCAAAATTACAAGGTTGG
CTGTCCACACCAAGATGGACTCAGGTTAAAGCTAGGCATTTTGTAAATATTGTTTTTGGGGTGACTTCATTTGTAGCTTT
CATATATTTAATAAGCGGTAACATTAGTTCGATTTGGCTTTTAGTTATAGCCTCTACTCTTTTAGGGATTGGGGTTACGT
TGCCGATTGGTGGAGCAGATATGCCTGTTGTCATATCTTTATTAAATAGCTATTCAGGTATTGCAGCTGCAGCTGCAGGT
TTCGTTGTAGATAGTCAGCTTTTGATTGTCGCTGGTGCAATGGTAGGAGCGGCCGGTTTAATTTTAACTCAGGTAATGTG
CAAAGGGATGAATAGGTCTCTGATTTCTGTCCTTTTTGGAGGTACTTTATCCCAGCAAAGTATTTCTTCTTCTGGATCAA
GTGAATACACAAATATAACTTCTTGTAGTGTTGAAGAATGCGCCTTAACATTAGAGGCTGCAAATAGGGTAATAGTCGTT
CCAGGTTATGGTCTTGCTGTCGCTCAAGCTCAACATACCTTGAGAGAAGTAACAAAAAAGCTAGAACAGAATGGAATAGA
AGTTGTATACGCAATTCATCCAGTAGCAGGCAGAATGCCAGGACATATGAATGTTTTACTAGCAGAAGCTGATGTTCCTT
ATGAACAGCTTAAAGAAATGGATATTGTTAATCCGGAATTCCCAGCAACAGATGTTGTTTTAGTTTTAGGTGCAAATGAT
GTAGTTAATCCTCAGGCAAAAAATGATAAAAGTTCTCCTCTGTATGGAATGCCTGTTCTTGATGTACAAGAAGCAAGAAC
TGTATTTGTTATCAAAAGAGGAATGAGTGCAGGTTATTCGGGCATTAAAAATGATTTATTTGATTTACCTAATACCTCAA
TGGTATTTGGGGATGCAAAAAAAGTATTAAGTGAGTTGATTTCAGAATTAAAGGATCTTGGCGTTGGTGATAAATAG

Upstream 100 bases:

>100_bases
TGGATTTGCTCTTTTCAATGTTGTAGGTGGTTTTTTTGTTACTGACAGAATGCTTGCCATGTTTAGTCGCAAACCTACAA
AAAATAAATAATTAATTGAT

Downstream 100 bases:

>100_bases
TAATATCTAGTTTGAATTTCTCTGATTATTTAAAGAAATACCCTTTTAATGAGGCTTTCCCTTGGTTAGGAGGAGATCTA
CAAACTTTAAGAGATACATT

Product: putative nicotinamide nucleotide transhydrogenase, subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNLPDIIKFVIDLLAVLLLALGIKGLSKVRSAREANILAAFAMFLSVVGLLSYYLGTSGIPAQSWIWIIVGSLVGSLFGT
LLAKKVPMTSMPETVALFNGFGGMSSLLVAIGAFLFPFNELAKAGSLEPFINNVSISVSIFVGAITFSGSIVAMAKLQGW
LSTPRWTQVKARHFVNIVFGVTSFVAFIYLISGNISSIWLLVIASTLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAAG
FVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLISVLFGGTLSQQSISSSGSSEYTNITSCSVEECALTLEAANRVIVV
PGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDIVNPEFPATDVVLVLGAND
VVNPQAKNDKSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLSELISELKDLGVGDK

Sequences:

>Translated_478_residues
MNLPDIIKFVIDLLAVLLLALGIKGLSKVRSAREANILAAFAMFLSVVGLLSYYLGTSGIPAQSWIWIIVGSLVGSLFGT
LLAKKVPMTSMPETVALFNGFGGMSSLLVAIGAFLFPFNELAKAGSLEPFINNVSISVSIFVGAITFSGSIVAMAKLQGW
LSTPRWTQVKARHFVNIVFGVTSFVAFIYLISGNISSIWLLVIASTLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAAG
FVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLISVLFGGTLSQQSISSSGSSEYTNITSCSVEECALTLEAANRVIVV
PGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDIVNPEFPATDVVLVLGAND
VVNPQAKNDKSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLSELISELKDLGVGDK
>Mature_478_residues
MNLPDIIKFVIDLLAVLLLALGIKGLSKVRSAREANILAAFAMFLSVVGLLSYYLGTSGIPAQSWIWIIVGSLVGSLFGT
LLAKKVPMTSMPETVALFNGFGGMSSLLVAIGAFLFPFNELAKAGSLEPFINNVSISVSIFVGAITFSGSIVAMAKLQGW
LSTPRWTQVKARHFVNIVFGVTSFVAFIYLISGNISSIWLLVIASTLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAAG
FVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLISVLFGGTLSQQSISSSGSSEYTNITSCSVEECALTLEAANRVIVV
PGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDIVNPEFPATDVVLVLGAND
VVNPQAKNDKSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLSELISELKDLGVGDK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=458, Percent_Identity=36.8995633187773, Blast_Score=291, Evalue=8e-79,
Organism=Homo sapiens, GI122939153, Length=458, Percent_Identity=36.8995633187773, Blast_Score=291, Evalue=8e-79,
Organism=Escherichia coli, GI1787886, Length=467, Percent_Identity=43.254817987152, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17550456, Length=407, Percent_Identity=40.5405405405405, Blast_Score=299, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50470; Mature: 50470

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPDIIKFVIDLLAVLLLALGIKGLSKVRSAREANILAAFAMFLSVVGLLSYYLGTSGI
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAQSWIWIIVGSLVGSLFGTLLAKKVPMTSMPETVALFNGFGGMSSLLVAIGAFLFPFNE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCHHH
LAKAGSLEPFINNVSISVSIFVGAITFSGSIVAMAKLQGWLSTPRWTQVKARHFVNIVFG
HHHCCCCCHHHCCEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VTSFVAFIYLISGNISSIWLLVIASTLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAAG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHHH
FVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLISVLFGGTLSQQSISSSGSSEYTNIT
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCC
SCSVEECALTLEAANRVIVVPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMP
CCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCC
GHMNVLLAEADVPYEQLKEMDIVNPEFPATDVVLVLGANDVVNPQAKNDKSSPLYGMPVL
CCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
DVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLSELISELKDLGVGDK
CHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLPDIIKFVIDLLAVLLLALGIKGLSKVRSAREANILAAFAMFLSVVGLLSYYLGTSGI
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAQSWIWIIVGSLVGSLFGTLLAKKVPMTSMPETVALFNGFGGMSSLLVAIGAFLFPFNE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCHHH
LAKAGSLEPFINNVSISVSIFVGAITFSGSIVAMAKLQGWLSTPRWTQVKARHFVNIVFG
HHHCCCCCHHHCCEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VTSFVAFIYLISGNISSIWLLVIASTLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAAG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHHH
FVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLISVLFGGTLSQQSISSSGSSEYTNIT
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCC
SCSVEECALTLEAANRVIVVPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMP
CCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCC
GHMNVLLAEADVPYEQLKEMDIVNPEFPATDVVLVLGANDVVNPQAKNDKSSPLYGMPVL
CCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
DVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLSELISELKDLGVGDK
CHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]