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The map label for this gene is rsuA [H]

Identifier: 123966487

GI number: 123966487

Start: 1102313

End: 1103035

Strand: Direct

Name: rsuA [H]

Synonym: P9515_12541

Alternate gene names: 123966487

Gene position: 1102313-1103035 (Clockwise)

Preceding gene: 123966482

Following gene: 123966489

Centisome position: 64.68

GC content: 30.29

Gene sequence:

>723_bases
ATGGCTACCAGAATAAATAAATATTTAAGTGAAGTAGGTTACTGTTCAAGAAGAGTAGCAGATAGATTAATTGAAGAAGG
AAAAGTAACCATTAATGGTAAAATTTCAGAAATAGGTACCAAGGTAAAGGAAGATGATCAAGTAGAAGTTGAAGGAATAA
TAATAGATAAACCAACTAAACAAAAATATATATACTTAGCCTTCAATAAACCTGTAGGAATTGTTTGCACAACCGATAGA
AGAGTTGAACCTGACAATATAATAGATTTCATCAATTATTCTACAAGAATTTTCCCCATTGGAAGATTGGATAAGAACAG
TGAGGGACTTATTTTTTTAACTAATGATGGTGATATCGTAAATAAAATACTTAGAGCAAGAAATAATCATGAAAAAGAAT
ACATAGTAAGCGTTAATCGCCCGATTAATAGAGACTTTATTCATAACATGAGTAATGGAGTTAAAATATTAGACACCATT
ACTAAGAATTGTTTCGTAAAACAATTGGGGGTAAAAAAATTTAAAATCATACTCACTCAAGGACTAAACCGTCAGATTAG
GCGAATGTGCGAGTCATTGGGCTACAGAGTACAATCTTTAAAACGTATAAGAATTATGAATATTAAGTTAGACATCCCAA
CTGGAAAATATCGCGAATTTACCAAAGATGAACTCTTAGAATTAAGTGGATTACTTGAAAACTCTTCAAAAACATTTAAC
TAA

Upstream 100 bases:

>100_bases
TTAACAACTATTATATGAATGCTAAATCAATCTATTAAAGTCATTACTTTTTTCGTGAAGTCAATTAAATTTAAAATAAA
TAAAAGGTAACTTTTTCAAA

Downstream 100 bases:

>100_bases
TTAAAAGTCTCTCTTTGTCGAGAGTTTAAAATTTTTTAATTTCAATTTTCTCCAAAACTATCAAAGAAAAAATTTTTTTT
ATTCTATCAGTAATAAAAAA

Product: putative pseudouridylate synthase specific to ribosomal small subunit

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MATRINKYLSEVGYCSRRVADRLIEEGKVTINGKISEIGTKVKEDDQVEVEGIIIDKPTKQKYIYLAFNKPVGIVCTTDR
RVEPDNIIDFINYSTRIFPIGRLDKNSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINRDFIHNMSNGVKILDTI
TKNCFVKQLGVKKFKIILTQGLNRQIRRMCESLGYRVQSLKRIRIMNIKLDIPTGKYREFTKDELLELSGLLENSSKTFN

Sequences:

>Translated_240_residues
MATRINKYLSEVGYCSRRVADRLIEEGKVTINGKISEIGTKVKEDDQVEVEGIIIDKPTKQKYIYLAFNKPVGIVCTTDR
RVEPDNIIDFINYSTRIFPIGRLDKNSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINRDFIHNMSNGVKILDTI
TKNCFVKQLGVKKFKIILTQGLNRQIRRMCESLGYRVQSLKRIRIMNIKLDIPTGKYREFTKDELLELSGLLENSSKTFN
>Mature_239_residues
ATRINKYLSEVGYCSRRVADRLIEEGKVTINGKISEIGTKVKEDDQVEVEGIIIDKPTKQKYIYLAFNKPVGIVCTTDRR
VEPDNIIDFINYSTRIFPIGRLDKNSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINRDFIHNMSNGVKILDTIT
KNCFVKQLGVKKFKIILTQGLNRQIRRMCESLGYRVQSLKRIRIMNIKLDIPTGKYREFTKDELLELSGLLENSSKTFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=58.0508474576271, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=29.3103448275862, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=28.755364806867, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27704; Mature: 27572

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRINKYLSEVGYCSRRVADRLIEEGKVTINGKISEIGTKVKEDDQVEVEGIIIDKPTK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCEEEEEEEEECCCC
QKYIYLAFNKPVGIVCTTDRRVEPDNIIDFINYSTRIFPIGRLDKNSEGLIFLTNDGDIV
CEEEEEEECCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCHHH
NKILRARNNHEKEYIVSVNRPINRDFIHNMSNGVKILDTITKNCFVKQLGVKKFKIILTQ
HHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHEEHHHHH
GLNRQIRRMCESLGYRVQSLKRIRIMNIKLDIPTGKYREFTKDELLELSGLLENSSKTFN
CCCHHHHHHHHHHCHHHHHCCEEEEEEEEEECCCCCCHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ATRINKYLSEVGYCSRRVADRLIEEGKVTINGKISEIGTKVKEDDQVEVEGIIIDKPTK
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCEEEEEEEEECCCC
QKYIYLAFNKPVGIVCTTDRRVEPDNIIDFINYSTRIFPIGRLDKNSEGLIFLTNDGDIV
CEEEEEEECCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCHHH
NKILRARNNHEKEYIVSVNRPINRDFIHNMSNGVKILDTITKNCFVKQLGVKKFKIILTQ
HHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHEEHHHHH
GLNRQIRRMCESLGYRVQSLKRIRIMNIKLDIPTGKYREFTKDELLELSGLLENSSKTFN
CCCHHHHHHHHHHCHHHHHCCEEEEEEEEEECCCCCCHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]