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The map label for this gene is lipA2 [H]

Identifier: 123966420

GI number: 123966420

Start: 1043259

End: 1044158

Strand: Reverse

Name: lipA2 [H]

Synonym: P9515_11871

Alternate gene names: 123966420

Gene position: 1044158-1043259 (Counterclockwise)

Preceding gene: 123966421

Following gene: 123966413

Centisome position: 61.27

GC content: 32.67

Gene sequence:

>900_bases
ATGAAAGATAAAAATCAAATTAAAATAGAAAAGATTTTAAGGCTTCCTTCTTGGATAAAATTTCCAATTAGTAAAGCCTC
TGAATTTGAGAAGATTCAAACACTTATAAAAAAATCGAATATTCATACTATTTGTGAAGAGGGAAGATGTCCAAATAGAG
CCGAATGTTATGCAGCAGGGACAGCTACTTTCTTACTGGGAGGCTCAATTTGTTCTCGCGCGTGCGCCTTCTGTCAGGTA
AATAAAGGTAAACCTACTCCCTTAAATAACTTCGAAAGCATTCAAGTCGCAGAAGCAGTAAAAATCCTAAATTTAAAATA
TGTCGTTTTAACCTCAGTCGCTAGAGATGATCTCCCCGATCATGGAGCTAATTTATTTATTTCAACGATTAATGAGATAA
GAAAGTTTGATCCAAAAATTCAAATTGAGGTACTAACCCCTGATTTATGGGGCGGCGGTAAAAGTTTTGAAGATAAAGAT
AAACTTCAAAAAGAGAGACTTAGAACCATACTAGAAAAAAAACCAACTTGTTTTAATCATAATCTTGAGACGGTTGAAAG
ACTTCAAAAAGAAGTTCGTAGAGGAGCTAGTTATAAAAATTCTTTAAGCTTGCTAGAAAAAGCTAAATCTATTGCTCCTG
ATATTCAAACTAAATCAGGAATTATGTTGGGTTTAGGAGAAACATTAAAGGAGATAGAAACAACAATTCTTGATCTAAAA
AAAGTAAATTGCGATCAAATTACAATTGGACAATACCTAAGACCTTCATTGAAGCATTTATCAGTTAAAAAATATTGGGA
ACCAAGTGAATTTGAATATCTAACAAATTTTTCCAAGAAATTAGGGTTTAAGAAAGTATCATGTGGACCTTTAGTTAGAA
GTAGTTATCACGCTGGTTGA

Upstream 100 bases:

>100_bases
CAAGAATTGCTTATGGGCTTCCGATGGGAAGCGAACTTGAATACGTTGATGAAGTTACTCTTGCTAGAGCTCTAGAAGGA
AGAACAAATTTAATTTAAAA

Downstream 100 bases:

>100_bases
GGCTTATTAATAGAAGAAAGTTTTTTTTCAATTTTATTTAAGATATATAAACTGTCTCCTGTCATTAGAGTTCCTGCCCC
TCCCCAAACTAACCTTAAAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKDKNQIKIEKILRLPSWIKFPISKASEFEKIQTLIKKSNIHTICEEGRCPNRAECYAAGTATFLLGGSICSRACAFCQV
NKGKPTPLNNFESIQVAEAVKILNLKYVVLTSVARDDLPDHGANLFISTINEIRKFDPKIQIEVLTPDLWGGGKSFEDKD
KLQKERLRTILEKKPTCFNHNLETVERLQKEVRRGASYKNSLSLLEKAKSIAPDIQTKSGIMLGLGETLKEIETTILDLK
KVNCDQITIGQYLRPSLKHLSVKKYWEPSEFEYLTNFSKKLGFKKVSCGPLVRSSYHAG

Sequences:

>Translated_299_residues
MKDKNQIKIEKILRLPSWIKFPISKASEFEKIQTLIKKSNIHTICEEGRCPNRAECYAAGTATFLLGGSICSRACAFCQV
NKGKPTPLNNFESIQVAEAVKILNLKYVVLTSVARDDLPDHGANLFISTINEIRKFDPKIQIEVLTPDLWGGGKSFEDKD
KLQKERLRTILEKKPTCFNHNLETVERLQKEVRRGASYKNSLSLLEKAKSIAPDIQTKSGIMLGLGETLKEIETTILDLK
KVNCDQITIGQYLRPSLKHLSVKKYWEPSEFEYLTNFSKKLGFKKVSCGPLVRSSYHAG
>Mature_299_residues
MKDKNQIKIEKILRLPSWIKFPISKASEFEKIQTLIKKSNIHTICEEGRCPNRAECYAAGTATFLLGGSICSRACAFCQV
NKGKPTPLNNFESIQVAEAVKILNLKYVVLTSVARDDLPDHGANLFISTINEIRKFDPKIQIEVLTPDLWGGGKSFEDKD
KLQKERLRTILEKKPTCFNHNLETVERLQKEVRRGASYKNSLSLLEKAKSIAPDIQTKSGIMLGLGETLKEIETTILDLK
KVNCDQITIGQYLRPSLKHLSVKKYWEPSEFEYLTNFSKKLGFKKVSCGPLVRSSYHAG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=41.156462585034, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=40.4669260700389, Blast_Score=196, Evalue=1e-50,
Organism=Escherichia coli, GI1786846, Length=291, Percent_Identity=43.298969072165, Blast_Score=257, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=311, Percent_Identity=40.8360128617363, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6324770, Length=304, Percent_Identity=40.1315789473684, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI221513272, Length=297, Percent_Identity=42.0875420875421, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33793; Mature: 33793

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKNQIKIEKILRLPSWIKFPISKASEFEKIQTLIKKSNIHTICEEGRCPNRAECYAAG
CCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHEEECC
TATFLLGGSICSRACAFCQVNKGKPTPLNNFESIQVAEAVKILNLKYVVLTSVARDDLPD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HGANLFISTINEIRKFDPKIQIEVLTPDLWGGGKSFEDKDKLQKERLRTILEKKPTCFNH
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
NLETVERLQKEVRRGASYKNSLSLLEKAKSIAPDIQTKSGIMLGLGETLKEIETTILDLK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
KVNCDQITIGQYLRPSLKHLSVKKYWEPSEFEYLTNFSKKLGFKKVSCGPLVRSSYHAG
HCCCCEEEHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MKDKNQIKIEKILRLPSWIKFPISKASEFEKIQTLIKKSNIHTICEEGRCPNRAECYAAG
CCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHEEECC
TATFLLGGSICSRACAFCQVNKGKPTPLNNFESIQVAEAVKILNLKYVVLTSVARDDLPD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HGANLFISTINEIRKFDPKIQIEVLTPDLWGGGKSFEDKDKLQKERLRTILEKKPTCFNH
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
NLETVERLQKEVRRGASYKNSLSLLEKAKSIAPDIQTKSGIMLGLGETLKEIETTILDLK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
KVNCDQITIGQYLRPSLKHLSVKKYWEPSEFEYLTNFSKKLGFKKVSCGPLVRSSYHAG
HCCCCEEEHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]