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The map label for this gene is prsA [H]

Identifier: 123966403

GI number: 123966403

Start: 1023824

End: 1024819

Strand: Reverse

Name: prsA [H]

Synonym: P9515_11701

Alternate gene names: 123966403

Gene position: 1024819-1023824 (Counterclockwise)

Preceding gene: 123966405

Following gene: 123966400

Centisome position: 60.14

GC content: 37.45

Gene sequence:

>996_bases
GTGACAAGTTTTATCACGGCAGTGGACAATGAAGTCCCAAATTTTAATCTAGCCAATAGTCGACTAAGGTTAGTCAGTGG
AACTTCTAATCCCAAATTAGCAGAAGAAATTGCTTCATATTTAGGGATAGAAAATGTTCCCTTAGTTTCTAAAAGATTTG
CTGATGGGGAACTTTATGTTCAAATTCAGCAATCTATAAGAGGTTGTGATGTATTCCTTATTCAGCCCACATGCGCTCCT
GTGAACGATAGTTTAATGGAGCTGATGATAATGGTAGATGCGTGCAAAAGAGCTTCAGCCAGGCAAATTACAGCTGTAAT
ACCCTATTTTGGATATGCTAGGGCAGATAGGAAAACCTCCGGAAGAGAATCAATTACAGCAAAGCTCACCGCAAATTTAC
TTGAAAAATCTGGTGTAGATAGAGTTCTTGCCATGGATTTACATTCTGCCCAAATACAAGGATATTTTGATATCCCTTGT
GATCATATTTATGGATCTCCAGTTCTGATTGATTATTTGGAAACTTTAAATTTGGAAGAAGTTGTAGTTGTATCTCCTGA
TGTGGGAGGTGTGGCCAGAGCAAGAGCATTTGCCAAATTAATGAAAGATGCTCCATTAGCCATTATTGATAAAAGAAGAT
CCGCTCACAATATTGCAGAGAGTTTGACTGTGATTGGAGAGGTAAAGGGGAAAACTGCTATTCTCATAGATGACATGATT
GATACTGGGGGAACAATTTGCTCAGGGGCAAATTTATTAAAGAAAGAAGGAGCAAAGAGAATTTTTGCATGTGCGTCACA
TGCGGTATTTTCTCCCCCATCTTATGAAAGATTAAGTGCAAAAGATTTGTTTGAACAAGTTATAGTAACTAACAGTATTC
CAGTTCTTGATAATAATAACTTCCAGCAGTTAAAAGTACTTTCTGTCGCTAATATGTTGGGAGAGGCAATTTGGAGGATT
CATGAAGAAAGCTCTGTTAGTTCTATGTTTAGATAA

Upstream 100 bases:

>100_bases
ATGGCACTTTCGACTTTAGAAGATAAAAAAAGTTAATTTATTTTTGCTAAATCGACATTAGGCACTACGATGAAAATAGA
TTTTTTTAAATGCTAATTTC

Downstream 100 bases:

>100_bases
TTAATTTATTTATTTATTTATTTTCTTTTAGAATTTCGTTAACTTTTTTCGTCACATCTTTCGGAACACTATCAGGACAA
TACTTTATTGCTGTAGTAAC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSFITAVDNEVPNFNLANSRLRLVSGTSNPKLAEEIASYLGIENVPLVSKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLIDYLETLNLEEVVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
DTGGTICSGANLLKKEGAKRIFACASHAVFSPPSYERLSAKDLFEQVIVTNSIPVLDNNNFQQLKVLSVANMLGEAIWRI
HEESSVSSMFR

Sequences:

>Translated_331_residues
MTSFITAVDNEVPNFNLANSRLRLVSGTSNPKLAEEIASYLGIENVPLVSKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLIDYLETLNLEEVVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
DTGGTICSGANLLKKEGAKRIFACASHAVFSPPSYERLSAKDLFEQVIVTNSIPVLDNNNFQQLKVLSVANMLGEAIWRI
HEESSVSSMFR
>Mature_330_residues
TSFITAVDNEVPNFNLANSRLRLVSGTSNPKLAEEIASYLGIENVPLVSKRFADGELYVQIQQSIRGCDVFLIQPTCAPV
NDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCD
HIYGSPVLIDYLETLNLEEVVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMID
TGGTICSGANLLKKEGAKRIFACASHAVFSPPSYERLSAKDLFEQVIVTNSIPVLDNNNFQQLKVLSVANMLGEAIWRIH
EESSVSSMFR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=42.4920127795527, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI4506129, Length=311, Percent_Identity=42.443729903537, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=41.85303514377, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=42.3566878980892, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI4506133, Length=354, Percent_Identity=35.0282485875706, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI194018537, Length=353, Percent_Identity=33.1444759206799, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=29.8611111111111, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=29.8611111111111, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=29.8611111111111, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=29.8611111111111, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=50.4823151125402, Blast_Score=326, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=42.6282051282051, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=42.3076923076923, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71989924, Length=318, Percent_Identity=41.8238993710692, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=43.1818181818182, Blast_Score=256, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17570245, Length=344, Percent_Identity=31.1046511627907, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320946, Length=318, Percent_Identity=40.8805031446541, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319403, Length=320, Percent_Identity=39.375, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321776, Length=313, Percent_Identity=38.3386581469649, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=42.1319796954315, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=98, Percent_Identity=37.7551020408163, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355239, Length=323, Percent_Identity=42.1052631578947, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45551540, Length=346, Percent_Identity=39.3063583815029, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24651458, Length=361, Percent_Identity=29.6398891966759, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651456, Length=361, Percent_Identity=29.6398891966759, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281362873, Length=361, Percent_Identity=29.6398891966759, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651454, Length=361, Percent_Identity=29.6398891966759, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651462, Length=189, Percent_Identity=35.4497354497354, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24651464, Length=189, Percent_Identity=35.4497354497354, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45552010, Length=189, Percent_Identity=35.4497354497354, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 36198; Mature: 36067

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFITAVDNEVPNFNLANSRLRLVSGTSNPKLAEEIASYLGIENVPLVSKRFADGELYV
CCCEEEHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHCCCCEEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTS
EEHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCCCCC
GRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLIDYLETLNLEE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCEEECCCHHHHCCCHHHHHHHHHCCCCE
VVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHEECCCCEEEEEECHH
DTGGTICSGANLLKKEGAKRIFACASHAVFSPPSYERLSAKDLFEQVIVTNSIPVLDNNN
CCCCCEECCCHHHHHCCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHCCCCEEECCC
FQQLKVLSVANMLGEAIWRIHEESSVSSMFR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
TSFITAVDNEVPNFNLANSRLRLVSGTSNPKLAEEIASYLGIENVPLVSKRFADGELYV
CCEEEHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHCCCCEEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTS
EEHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCCCCC
GRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLIDYLETLNLEE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCEEECCCHHHHCCCHHHHHHHHHCCCCE
VVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHEECCCCEEEEEECHH
DTGGTICSGANLLKKEGAKRIFACASHAVFSPPSYERLSAKDLFEQVIVTNSIPVLDNNN
CCCCCEECCCHHHHHCCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHCCCCEEECCC
FQQLKVLSVANMLGEAIWRIHEESSVSSMFR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]