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The map label for this gene is cobB [H]

Identifier: 123966395

GI number: 123966395

Start: 1015192

End: 1016718

Strand: Reverse

Name: cobB [H]

Synonym: P9515_11621

Alternate gene names: 123966395

Gene position: 1016718-1015192 (Counterclockwise)

Preceding gene: 123966396

Following gene: 123966391

Centisome position: 59.66

GC content: 30.12

Gene sequence:

>1527_bases
ATGATTTTTCTTCATCAGAATTTGATGTTAGTAAGTTATTGGGGAGTAGTAGAGGAAATACTAGCCCACTTTTTGAAAAT
GCTATTAAGGTTGCAGTTCAAATATTTAATAGTCTTTGTAAATAAAAGTAATTTAATGTCTTGTGTAATATCTTCTCCTT
CAACTGATAGTGGAAAAACTACTTTATCGCTGTTAATTTCTTGTTGGGCTTTTTCAAAAGGTATAGAAATACAAACTTTT
AAAATCGGGCCAGATTATCTTGATCAACAACAAATGAGTTCAATTGGCCAACCTATTTGTAGGAATTTAGATATTTTTTT
AAGCGGTGAAGAATGGGTTAGAAATAATTTCTTAAAGCATTCTATGAAATATGAATTTTCATTAATTGAAGGAGCAATGG
GGCTTTTTGATGGGTTAGGGGCGACTTCATATTCAAGTACTGCAAATGTTGCTAAGCTTCTTGATACTCCAATAATTTTT
ATTGTTAATGCAAGAGGTCAAGTGGCCTCTCTTTTACCAACCCTAAGAGGTTTCAGAGATTTTGATAAAGGATTATCGAT
AAAAGGAATTATATTCAATAACGTCAATTCAGATAGACATAAAAGTTTAATTAGAGAAGTTTTTAAAAATGAAGATATAG
AAATTCTTGGATTTTTACCCTCAGATTCAAAAATAACTGTTAGAAAAGCAAATTTAGGTTTAGTTTCTCCATTTGATGAT
GCTAGAAAAATTGATGTTGACTATTTTGCAAGTTTCGCTCAAGAAAACCTTGATGTATTTTCTCTAATCAAATTTCTGAA
AGCTCCTTCAGAAAAAATATTTAATACATCTTTTGGTAAAAATTTTAATATAGATAAAAACAAACCTATCGCAATAGCTG
AAGATAGAATCTTTCATTTCCAATACACTGAAACGAAGGAGTTTCTTCATGAAATGGGAATACCTCTACTTTCATGGAGC
ATATATAATAATGAAGAAATTCCACAGGAAGCTTCATCTTTAATTATTCCTGGAGGCTTTCCAGAAAAATATGCAAGTCA
CATAAGTGATTCTGATAAAAGCTTAAATTCATTAAGGGAATTCCGAAAAAAAGGATTTATTTATGCAGAGTGTGGTGGGA
TGATGATTTTAGGAGATTTTATAAAAGATGAAAATGGTGACAATCATAAAATGAGCGGAATACTACCTTTTAAATCAAAA
AAAAGTAATTTATCTGTAGGGTATAGATATATAAAAGGTTTAAAAGATTCCCCAATTATTAAAAAAAATCAATCAATTAG
AGGACATGAATTTCATTATTGGGAAATTGAAAGTTATTCATCAGAAGTAGATTTAAAGAAAACAAAGCATAAGAACAAAT
TCACTTCACCATGGGAAATAAAATCGTGGGAGACTGAATTTAAAAATGAAGGTTGGTCAGATAAAAAATTACACGCAAGT
TGGATACACCTACATTTACCAAGTTGCCCAGAAGCTGCAAGAAACTTTCTAGAAGCTACACAAAATGATTATTCTAGGAA
TTCCTAA

Upstream 100 bases:

>100_bases
AATAATACTTGTGTAATTCTTGGATGTGAATCTGTTGAATGTATGAGACTTGAAGCCGGAGGTATGGCTGATATGCAATT
AATAGAACAAGTTGTCCCAA

Downstream 100 bases:

>100_bases
TTTATTATCAAATCTAATATATGGAGAGGAGAACCTATATATACTATTCCTGGTAATGTAATTAGTGGGCCTAGAAGACT
GCCTACAATGACTTCAGATT

Product: putative cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MIFLHQNLMLVSYWGVVEEILAHFLKMLLRLQFKYLIVFVNKSNLMSCVISSPSTDSGKTTLSLLISCWAFSKGIEIQTF
KIGPDYLDQQQMSSIGQPICRNLDIFLSGEEWVRNNFLKHSMKYEFSLIEGAMGLFDGLGATSYSSTANVAKLLDTPIIF
IVNARGQVASLLPTLRGFRDFDKGLSIKGIIFNNVNSDRHKSLIREVFKNEDIEILGFLPSDSKITVRKANLGLVSPFDD
ARKIDVDYFASFAQENLDVFSLIKFLKAPSEKIFNTSFGKNFNIDKNKPIAIAEDRIFHFQYTETKEFLHEMGIPLLSWS
IYNNEEIPQEASSLIIPGGFPEKYASHISDSDKSLNSLREFRKKGFIYAECGGMMILGDFIKDENGDNHKMSGILPFKSK
KSNLSVGYRYIKGLKDSPIIKKNQSIRGHEFHYWEIESYSSEVDLKKTKHKNKFTSPWEIKSWETEFKNEGWSDKKLHAS
WIHLHLPSCPEAARNFLEATQNDYSRNS

Sequences:

>Translated_508_residues
MIFLHQNLMLVSYWGVVEEILAHFLKMLLRLQFKYLIVFVNKSNLMSCVISSPSTDSGKTTLSLLISCWAFSKGIEIQTF
KIGPDYLDQQQMSSIGQPICRNLDIFLSGEEWVRNNFLKHSMKYEFSLIEGAMGLFDGLGATSYSSTANVAKLLDTPIIF
IVNARGQVASLLPTLRGFRDFDKGLSIKGIIFNNVNSDRHKSLIREVFKNEDIEILGFLPSDSKITVRKANLGLVSPFDD
ARKIDVDYFASFAQENLDVFSLIKFLKAPSEKIFNTSFGKNFNIDKNKPIAIAEDRIFHFQYTETKEFLHEMGIPLLSWS
IYNNEEIPQEASSLIIPGGFPEKYASHISDSDKSLNSLREFRKKGFIYAECGGMMILGDFIKDENGDNHKMSGILPFKSK
KSNLSVGYRYIKGLKDSPIIKKNQSIRGHEFHYWEIESYSSEVDLKKTKHKNKFTSPWEIKSWETEFKNEGWSDKKLHAS
WIHLHLPSCPEAARNFLEATQNDYSRNS
>Mature_508_residues
MIFLHQNLMLVSYWGVVEEILAHFLKMLLRLQFKYLIVFVNKSNLMSCVISSPSTDSGKTTLSLLISCWAFSKGIEIQTF
KIGPDYLDQQQMSSIGQPICRNLDIFLSGEEWVRNNFLKHSMKYEFSLIEGAMGLFDGLGATSYSSTANVAKLLDTPIIF
IVNARGQVASLLPTLRGFRDFDKGLSIKGIIFNNVNSDRHKSLIREVFKNEDIEILGFLPSDSKITVRKANLGLVSPFDD
ARKIDVDYFASFAQENLDVFSLIKFLKAPSEKIFNTSFGKNFNIDKNKPIAIAEDRIFHFQYTETKEFLHEMGIPLLSWS
IYNNEEIPQEASSLIIPGGFPEKYASHISDSDKSLNSLREFRKKGFIYAECGGMMILGDFIKDENGDNHKMSGILPFKSK
KSNLSVGYRYIKGLKDSPIIKKNQSIRGHEFHYWEIESYSSEVDLKKTKHKNKFTSPWEIKSWETEFKNEGWSDKKLHAS
WIHLHLPSCPEAARNFLEATQNDYSRNS

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 58077; Mature: 58077

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLHQNLMLVSYWGVVEEILAHFLKMLLRLQFKYLIVFVNKSNLMSCVISSPSTDSGKT
CEEEECCEEEHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCEEEEEECCCCCCCCHH
TLSLLISCWAFSKGIEIQTFKIGPDYLDQQQMSSIGQPICRNLDIFLSGEEWVRNNFLKH
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEEEECCHHHHHHHHHHH
SMKYEFSLIEGAMGLFDGLGATSYSSTANVAKLLDTPIIFIVNARGQVASLLPTLRGFRD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
FDKGLSIKGIIFNNVNSDRHKSLIREVFKNEDIEILGFLPSDSKITVRKANLGLVSPFDD
HHCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEECCCCC
ARKIDVDYFASFAQENLDVFSLIKFLKAPSEKIFNTSFGKNFNIDKNKPIAIAEDRIFHF
CCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEECCEEEEE
QYTETKEFLHEMGIPLLSWSIYNNEEIPQEASSLIIPGGFPEKYASHISDSDKSLNSLRE
EEHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCEEECCCCHHHHHHHCCCCHHHHHHHHH
FRKKGFIYAECGGMMILGDFIKDENGDNHKMSGILPFKSKKSNLSVGYRYIKGLKDSPII
HHHCCCEEEECCCEEEEEHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCC
KKNQSIRGHEFHYWEIESYSSEVDLKKTKHKNKFTSPWEIKSWETEFKNEGWSDKKLHAS
CCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
WIHLHLPSCPEAARNFLEATQNDYSRNS
EEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFLHQNLMLVSYWGVVEEILAHFLKMLLRLQFKYLIVFVNKSNLMSCVISSPSTDSGKT
CEEEECCEEEHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCEEEEEECCCCCCCCHH
TLSLLISCWAFSKGIEIQTFKIGPDYLDQQQMSSIGQPICRNLDIFLSGEEWVRNNFLKH
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEEEECCHHHHHHHHHHH
SMKYEFSLIEGAMGLFDGLGATSYSSTANVAKLLDTPIIFIVNARGQVASLLPTLRGFRD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
FDKGLSIKGIIFNNVNSDRHKSLIREVFKNEDIEILGFLPSDSKITVRKANLGLVSPFDD
HHCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEECCCCC
ARKIDVDYFASFAQENLDVFSLIKFLKAPSEKIFNTSFGKNFNIDKNKPIAIAEDRIFHF
CCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEECCEEEEE
QYTETKEFLHEMGIPLLSWSIYNNEEIPQEASSLIIPGGFPEKYASHISDSDKSLNSLRE
EEHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCEEECCCCHHHHHHHCCCCHHHHHHHHH
FRKKGFIYAECGGMMILGDFIKDENGDNHKMSGILPFKSKKSNLSVGYRYIKGLKDSPII
HHHCCCEEEECCCEEEEEHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCC
KKNQSIRGHEFHYWEIESYSSEVDLKKTKHKNKFTSPWEIKSWETEFKNEGWSDKKLHAS
CCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
WIHLHLPSCPEAARNFLEATQNDYSRNS
EEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]