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The map label for this gene is gatA

Identifier: 123966262

GI number: 123966262

Start: 921619

End: 923073

Strand: Reverse

Name: gatA

Synonym: P9515_10291

Alternate gene names: 123966262

Gene position: 923073-921619 (Counterclockwise)

Preceding gene: 123966263

Following gene: 123966261

Centisome position: 54.17

GC content: 34.91

Gene sequence:

>1455_bases
ATGAACTTTAATTCTTTTCGAAAGGAAATTAAAAGTGGAAATGCCTCAGTTAAGGAGTTAATTAATGAATTTTTTTTGAA
AATTGATTCATTAGATCCAAAAATAAATGCCTATACTTGCTTAACAAAAGAAATAGCGAACTCTCAATCTGAACAAATAG
ATAAATTAATTAGTAATAATGAACAATTACCGCCTCTTGCAGGAATACCCATTGCGATAAAAGATAATATTTGTACTAAA
GGAGTAGTAACCTCTTGTTCAAGTAAAATGCTTCAAGACTTTGTTTCTCCATACGAGTCTACTGTCTCAGGTAAATTATG
GTCATCGGGTGGTATTTTTCTTGGAAAAACTAATCTTGATGAATTCGCAATGGGCAGTTCTACAGAGACTTCCGTATTTG
GTACAACTTCAAACCCTTGGGATACAAAAAGAGTTCCAGGAGGTAGTTCTGGGGGTAGCGCTGCTTCAGTAGCTGCTGGT
TTATGTGCAGCCTCTATTGGTTCTGATACTGGGGGATCTATCAGACAGCCTGCATCTTTTTGTGGTGTAGTGGGTTTGAA
ACCCACCTATGGAAGAGTCAGTAGATGGGGATTAATTGCTTTTGCAAGCTCTCTAGATCAAATTGGTCCAATTACAAATA
CTGTTTCTGATGCAGCTGAAATACTATATTCCATGTCCGGAAAGGATCCTCTTGATTCTACTTGCCTTGATAAACCAGTA
CCAAATTATTTATCTGATTTGGATAAATCTATTAAAGGTTTAAAAATTGGAATTATTGAAGAATGCTTTAATCATCCTGG
ACTTGATCAAGAAGTCAAATTATCTGTTTTATCAGCTGTAGATAGACTCAAATCTCTGGGTGCTGAAATTCATGATGTAA
AATGTCCAAGATTTAATGATGGAATAGCAACTTATTATGTAATAGCTCCATGTGAGGCCTCTGCTAATTTGGCAAGATAT
GATGGAGTTAAATATGGATATAGATCAGATGGAGATACTAATTTAGTAGAAATGATCTCTAAAAGTAGAGCAGAAGGCTT
TGGAAATGAAGTACAAAGAAGAATTTTGATTGGTACGTATGCTTTATCTGCCGGATATAGTGATGCTTATTATAAGAAAG
CTCAGAGAGTAAGAACTTTAATTAGAAAGGATTTTGATAGTGCTTTTGATAAGGTAGATGTTTTATTAACCCCTACATGC
CCTACTACTGCATTCTTGAAAGGTGATTTTATAAACGATCCTTTATCTATGTATTTGTCAGATCTTTTAACTGTTCCAGC
TAATCTTGCAGGGCTACCAGCAATTAGTATTCCATGTGGATTTGATAAAAAAGGCTTACCTATTGGTCTTCAATTAATCG
GGAATGTTTTGGAGGAGCATCGAATATTAAACGTCGCAAATATATTTGAAAAGGATGCTGACGTCATGAAGAGAAAACCT
AGTATTGAAATTTGA

Upstream 100 bases:

>100_bases
TGAGGGTTCAATAGATATTCAACATAGTATTATTAGATGTAAAAGAGAAGAACCATTAACTGTTTGATAATTAAATTAAT
CTCAAAAACTCTATCTTGAT

Downstream 100 bases:

>100_bases
ATTAATAATTAATTTGTGTGAGTTAAACATAGAAATTTTTAAAATATTCTCTATCTTGTAAATATAAATTGTTTAAATAT
GGGATTCGTTCCACTTCATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNFNSFRKEIKSGNASVKELINEFFLKIDSLDPKINAYTCLTKEIANSQSEQIDKLISNNEQLPPLAGIPIAIKDNICTK
GVVTSCSSKMLQDFVSPYESTVSGKLWSSGGIFLGKTNLDEFAMGSSTETSVFGTTSNPWDTKRVPGGSSGGSAASVAAG
LCAASIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLIAFASSLDQIGPITNTVSDAAEILYSMSGKDPLDSTCLDKPV
PNYLSDLDKSIKGLKIGIIEECFNHPGLDQEVKLSVLSAVDRLKSLGAEIHDVKCPRFNDGIATYYVIAPCEASANLARY
DGVKYGYRSDGDTNLVEMISKSRAEGFGNEVQRRILIGTYALSAGYSDAYYKKAQRVRTLIRKDFDSAFDKVDVLLTPTC
PTTAFLKGDFINDPLSMYLSDLLTVPANLAGLPAISIPCGFDKKGLPIGLQLIGNVLEEHRILNVANIFEKDADVMKRKP
SIEI

Sequences:

>Translated_484_residues
MNFNSFRKEIKSGNASVKELINEFFLKIDSLDPKINAYTCLTKEIANSQSEQIDKLISNNEQLPPLAGIPIAIKDNICTK
GVVTSCSSKMLQDFVSPYESTVSGKLWSSGGIFLGKTNLDEFAMGSSTETSVFGTTSNPWDTKRVPGGSSGGSAASVAAG
LCAASIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLIAFASSLDQIGPITNTVSDAAEILYSMSGKDPLDSTCLDKPV
PNYLSDLDKSIKGLKIGIIEECFNHPGLDQEVKLSVLSAVDRLKSLGAEIHDVKCPRFNDGIATYYVIAPCEASANLARY
DGVKYGYRSDGDTNLVEMISKSRAEGFGNEVQRRILIGTYALSAGYSDAYYKKAQRVRTLIRKDFDSAFDKVDVLLTPTC
PTTAFLKGDFINDPLSMYLSDLLTVPANLAGLPAISIPCGFDKKGLPIGLQLIGNVLEEHRILNVANIFEKDADVMKRKP
SIEI
>Mature_484_residues
MNFNSFRKEIKSGNASVKELINEFFLKIDSLDPKINAYTCLTKEIANSQSEQIDKLISNNEQLPPLAGIPIAIKDNICTK
GVVTSCSSKMLQDFVSPYESTVSGKLWSSGGIFLGKTNLDEFAMGSSTETSVFGTTSNPWDTKRVPGGSSGGSAASVAAG
LCAASIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLIAFASSLDQIGPITNTVSDAAEILYSMSGKDPLDSTCLDKPV
PNYLSDLDKSIKGLKIGIIEECFNHPGLDQEVKLSVLSAVDRLKSLGAEIHDVKCPRFNDGIATYYVIAPCEASANLARY
DGVKYGYRSDGDTNLVEMISKSRAEGFGNEVQRRILIGTYALSAGYSDAYYKKAQRVRTLIRKDFDSAFDKVDVLLTPTC
PTTAFLKGDFINDPLSMYLSDLLTVPANLAGLPAISIPCGFDKKGLPIGLQLIGNVLEEHRILNVANIFEKDADVMKRKP
SIEI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=463, Percent_Identity=41.036717062635, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI195972892, Length=485, Percent_Identity=26.3917525773196, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI166795287, Length=225, Percent_Identity=28.4444444444444, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=410, Percent_Identity=39.7560975609756, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=482, Percent_Identity=25.7261410788382, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538252, Length=318, Percent_Identity=27.9874213836478, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=302, Percent_Identity=31.4569536423841, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=193, Percent_Identity=34.7150259067358, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538254, Length=263, Percent_Identity=26.9961977186312, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=173, Percent_Identity=35.8381502890173, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=173, Percent_Identity=35.8381502890173, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=420, Percent_Identity=36.9047619047619, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=463, Percent_Identity=31.7494600431965, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=193, Percent_Identity=32.6424870466321, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=44.078947368421, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24644968, Length=489, Percent_Identity=26.7893660531697, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45550774, Length=499, Percent_Identity=25.250501002004, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648435, Length=499, Percent_Identity=25.250501002004, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648437, Length=499, Percent_Identity=25.250501002004, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=24.8979591836735, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=24.8979591836735, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=24.8979591836735, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=24.8979591836735, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=27.5793650793651, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648441, Length=443, Percent_Identity=25.7336343115124, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24648439, Length=443, Percent_Identity=25.7336343115124, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21356731, Length=196, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PROM5 (A2BWS5)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011343.1
- ProteinModelPortal:   A2BWS5
- SMR:   A2BWS5
- STRING:   A2BWS5
- GeneID:   4719177
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_10291
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   PMAR167542:P9515ORF_1077-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52121; Mature: 52121

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 74-74 ACT_SITE 149-149 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNSFRKEIKSGNASVKELINEFFLKIDSLDPKINAYTCLTKEIANSQSEQIDKLISNN
CCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
EQLPPLAGIPIAIKDNICTKGVVTSCSSKMLQDFVSPYESTVSGKLWSSGGIFLGKTNLD
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCH
EFAMGSSTETSVFGTTSNPWDTKRVPGGSSGGSAASVAAGLCAASIGSDTGGSIRQPASF
HHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLIAFASSLDQIGPITNTVSDAAEILYSMSGKDPLDSTCLDKPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCC
PNYLSDLDKSIKGLKIGIIEECFNHPGLDQEVKLSVLSAVDRLKSLGAEIHDVKCPRFND
HHHHHHHHHHHCCEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
GIATYYVIAPCEASANLARYDGVKYGYRSDGDTNLVEMISKSRAEGFGNEVQRRILIGTY
CCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHEEHH
ALSAGYSDAYYKKAQRVRTLIRKDFDSAFDKVDVLLTPTCPTTAFLKGDFINDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDKKGLPIGLQLIGNVLEEHRILNVANIFEKDADVMKRKP
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SIEI
CCCC
>Mature Secondary Structure
MNFNSFRKEIKSGNASVKELINEFFLKIDSLDPKINAYTCLTKEIANSQSEQIDKLISNN
CCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
EQLPPLAGIPIAIKDNICTKGVVTSCSSKMLQDFVSPYESTVSGKLWSSGGIFLGKTNLD
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCH
EFAMGSSTETSVFGTTSNPWDTKRVPGGSSGGSAASVAAGLCAASIGSDTGGSIRQPASF
HHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLIAFASSLDQIGPITNTVSDAAEILYSMSGKDPLDSTCLDKPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCC
PNYLSDLDKSIKGLKIGIIEECFNHPGLDQEVKLSVLSAVDRLKSLGAEIHDVKCPRFND
HHHHHHHHHHHCCEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
GIATYYVIAPCEASANLARYDGVKYGYRSDGDTNLVEMISKSRAEGFGNEVQRRILIGTY
CCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHEEHH
ALSAGYSDAYYKKAQRVRTLIRKDFDSAFDKVDVLLTPTCPTTAFLKGDFINDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDKKGLPIGLQLIGNVLEEHRILNVANIFEKDADVMKRKP
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SIEI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA